Study of Cell Compatibility for Using Acellular Frame as Substitute Bile Duct_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

JIANG Xiaoqiong ¹ , XIONG Xianze ² , CHENG Nansheng ² , CHENG Jingqiu ¹ , ZHANG Jie ¹

1.Key Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China;;
2.Department of Hepatobiliary Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

Acellular matrix; Human intrahepatic bile duct endothelial cell; Cytotoxicity; Cell compatibility

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Objective To investigate the cell compatibility of the porcine acellular lumens matrix substituting bile duct and evaluate the method to guide the clinical application of the porcine lumens scaffold.
Methods Porcine bile duct and ureter were treated using detergent sodium dodecylsulphate (SDS) and 1% Triton X-100 to prepare the acellular lumens matrix. The toxic effects of different concentrations of acellular lumens matrix extract were tested by MTT to assess the proliferation of human scarfskin fibroblasts (HSF). The cytotoxicity of the target biomaterial was graded according to the national standards. The growth manner of the human intrahepatic bile duct endothelial cells (HIBDCs) seeded on the acellular lumens matrix was studied after 20 d under scanning electron microscopy.
Results Acellular lumens matrix was completely devoid of cellular and nuclear material while maintaining the integrity of extracellular collagenous matrix. The cytotoxicity score of the matrix was in grade 0－1, which meant the biomaterial had no cytotoxicity. The microscopy showed the seeded HIBDCs had the potentials of spread and proliferation on the matrix, but there were few cells infiltrating into the acellular lumens matrix.
Conclusions Porcine acellular lumens matrix is a natural non-toxic xenogenic lumens substitute with good cell affinity, but the time of adherence is long, so further endeavors are needed to improve the progress of adherence.

Citation： JIANG Xiaoqiong ,XIONG Xianze,CHENG Nansheng,CHENG Jingqiu,ZHANG Jie. Study of Cell Compatibility for Using Acellular Frame as Substitute Bile Duct. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(3): 248-252. doi: Copy

1.	刘松阳, 王广义, 刘凯. 人工胆管替代物的设计与发展 [J]. 中国组织工程研究与临床康复, 2007； 11(12): 22952298.
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25.	杨晓芳. 生物材料生物相容性评价研究进展 [J]. 生物医学工程学杂志, 2001; 18(1): 123128.

1. 刘松阳, 王广义, 刘凯. 人工胆管替代物的设计与发展 [J]. 中国组织工程研究与临床康复, 2007； 11(12): 22952298.
2. Ott HC, Matthiesen TS, Goh SK, et al. Perfusiondecellularized matrix: using nature’s platform to engineer a bioartificial heart [J]. Nat Med, 2008; 14(2): 213221.
3. Yang J, Yamato M, Shimizu T, et al. Reconstruction of functional tissues with cell sheet engineering [J]. Biomaterials, 2007; 28(34): 50335043.
4. Malafaya PB, Silva GA, Reis RL.Naturalorigin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications [J]. Adv Drug Deliv Rev, 2007; 59(45): 207233.
5. Leor J, Amsalem Y, Cohen S. Cells, scaffolds, and molecules for myocardial tissue engineering [J]. Pharmacol Ther, 2005; 105(2): 151163.
6. Bolland F, Korossis S, Wilshaw SP, et al. Development and characterisation of a fullthickness acellular porcine bladder matrix for tissue engineering [J]. Biomaterials, 2007; 28(6): 10611070.
7. Badylak SF, Kochupura PV, Cohen IS, et al. The use of extracellular matrix as an inductive scaffold for the partial replacement of functional myocardium [J]. Cell Transplant, 2006; 15 Suppl 1: S29S40.
8. Gilbert TW, Gilbert S, Madden M, et al. Morphologic assessment of extracellular matrix scaffolds for patch tracheoplasty in a canine model [J]. Ann Thorac Surg, 2008; 86(3): 967974.
9. Kwon TG, Yoo JJ, Atala A. Autologous penile corpora cavernosa replacement using tissue engineering techniques [J]. J Urol, 2002; 168(4 Pt 2): 17541758.
10. Conconi MT, De Coppi P, Di Liddo R, et al. Tracheal matrices, obtained by a detergentenzymatic method, support in vitro the adhesion of chondrocytes and tracheal epithelial cells [J]. Transpl Int, 2005; 18(6): 727734.
11. Biological evaluation of medical device—Part 12: Sample preparation and reference materials [S]. GB/T16886.1202005/ISO1099312: 2002.
12. Sahlin H, Nygren H. Cytotoxicity testing of wounddressing materials [J]. Altern Lab Anim, 2001; 29(3): 269275.
13. Macchiarini P, Jungebluth P, Go T, et al. Clinical transplantation of a tissueengineered airway [J]. Lancet, 2008; 372(9655): 20232030.
14. Cebotari S, Lichtenberg A, Tudorache I, et al. Clinical application of tissue engineered human heart valves using autologous progenitor cells [J]. Circulation, 2006; 114(1 Suppl): 2006; 114: Ⅰ132Ⅰ137.
15. 郑建伟，邹声泉. 人工胆管研究 [J]. 中国医学工程, 2005; 13(4): 385387, 390.
16. 李海洋, 孙诚谊, 潘耀振, 等. 不同途径胆道支架置入治疗恶性梗阻性黄疸42例回顾性分析 [J]. 中国普外基础与临床杂志, 2009; 16(4): 300303.
17. 刘伟, 卢武胜. 胆管内支架治疗梗阻性黄疸的临床应用 [J]. 中国普外基础与临床杂志, 2007; 14(4): 490492.
18. 王蒙, 王广义, 张平, 等. 胆道镜下气囊扩张治疗胆道术后肝内外胆管狭窄 [J]. 中国普外基础与临床杂志, 2009; 16(11): 930931.
19. 张文斌, 吴汉江. 无细胞胶原基质的研究进展 [J]. 国外医学生物医学工程分册， 2004； 27(2): 127128.
20. Simon P, Kasimir MT, Seebacher G, et al. Early failure of the tissue engineered porcine heart valve SYNERGRAFT in pediatric patients [J]. Eur J Cardiothorac Surg, 2003; 23(6): 10021006.
21. Cebotari S, Mertsching H, Kallenbach K, et al. Construction of autologous human heart valves based on an acellular allograft matrix [J]. Circulation, 2002; 106(12 Suppl 1): Ⅰ63Ⅰ68.
22. Meyers RL, Lowichik A, Kraiss LW, et al. Aortoiliac reconstruction in infants and toddlers: replacement with decellularized branched pulmonary artery allograft [J]. J Pediatr Surg, 2006; 41(1): 226229.
23. Bernatchez SF, Parks PJ, Gibbons DF. Interaction of macrophages with fibrous materials in vitro [J]. Biomaterials, 1996; 17(21): 20772086.
24. Furmanb K, Periosteum HS. Its biomechanical role in bone fracture (Department of Bioengineering) [M]. Nacob: Clemson University Clemson, 1998: 1418.
25. 杨晓芳. 生物材料生物相容性评价研究进展 [J]. 生物医学工程学杂志, 2001; 18(1): 123128.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study of Cell Compatibility for Using Acellular Frame as Substitute Bile Duct

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