Role of Vascular Endothelial Growth Factor in Protection on Intrahepatic Cholangiocyte Mediated by Hypoxic Preconditioning after Liver Graft_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHOU Bin , ZHANG Peijian , LI Yong , TIAN Tian , JIN Cheng , LIU Xinyan , FENG Min , LANG Jie.

Institute of General Surgical Research, No.2 Clinical Medical College, Yangzhou 225001, Jiangsu Province, China;

Corresponding author：

Keywords：

Vascular endothelial growth factor; Hypoxic preconditioning; Liver transplantation; Cholangiocyte

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the protection on the intrahepatic cholangiocyte mediated by hypoxic preconditioning (HP) after liver transplantation and the role of vascular endothelial growth factor (VEGF). Methods The model of autologous liver transplantation was established, and the rats were divided into 3 groups: autologous liver transplantation group, hypoxic preconditioning before operation group (HP group) and sham operation group. At 6, 12, 24, 48 h after operation, blood samples were collected for examination of the serum total bilirubin (TBIL), direct bilirubin (DBIL) and alkaline phosphatase (ALP), and the expression of VEGF was detected by immunohistochemical method. The pathological changes of cholangiocytes were observed by light microscope. Results As compared with autologous liver transplantation group, the levels of seurm TBIL, DBIL and ALP in HP group were lower (P＜0.05), while the expression of VEGF in HP group was higher at the whole process (P＜0.05). The degrees of billiary epithelium damage and inflammatory infiltration in autologous liver transplantation group were more severe than those in HP group. Conclusion HP has protective effect on cholangiocytes after liver transplantation, in which VEGF may play an important role.

Citation： ZHOU Bin,ZHANG Peijian,LI Yong,TIAN Tian,JIN Cheng,LIU Xinyan,FENG Min,LANG Jie.. Role of Vascular Endothelial Growth Factor in Protection on Intrahepatic Cholangiocyte Mediated by Hypoxic Preconditioning after Liver Graft. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(5): 365-369. doi: Copy

1.	Wang Y, Mao XO, Xie L, et al. Vascular endothelial growth factor overexpression delays neurodegeneration and prolongs survival in amyotrophic lateral sclerosis mice [J]. J Neurosci, 2007; 27 (2): 304-307.
2.	Li LH, Guo ZJ, Yan LL, et al． Antitumor and antiangiogenic activities of anti-vascular endothelial growth factor hairpin ribozyme in human hepatocellular carcinoma cell cultures and xenografts [J]. World J Gastroenterol, 2007; 13(47): 6425-6432.
3.	Stroka DM, Burkhardt T, Desbaillets I, et al. HIF-1 is expressed in normoxic tissue and displays an organ specific regulation under systemic hypoxia [J]. FASEB J, 2001; 15(13): 2445-2453.
4.	陶立德，张培建, 田明祥, 等. 低氧预适应对移植肝脏肝窦内皮细胞损伤的影响 [J]. 实用临床医药杂志, 2007; 11(2): 39-42.
5.	赵宏峰, 周杰. 大鼠自体原位肝移植胆道缺血再灌注损伤模型的建立 [J]. 第二军医大学学报, 2006； 27(4): 429-430.
6.	梁四海, 陶立德, 张培建, 等. 低氧预适应对大鼠移植肝脏功能和组织结构的影响 [J]. 江苏大学学报(医学版), 2007; 17(3): 219-221.
7.	张培建, 卜平, 金成. 低氧预适应对移植大鼠肝细胞线粒体超微结构的影响 [J]. 解剖学报, 2008; 39(6): 854-857.
8.	孔连宝, 吴晓峰, 邵立军, 等. 吻合肝动脉对大鼠部分肝移植早期损伤的影响 [J]. 中华实验外科杂志, 2006; 23(5): 543-545.
9.	彭方兴, 郑本波, 刘立新, 等. 大鼠原位肝移植动物模型制作要点 [J]. 中国普外基础与临床杂志, 2005; 12(2）: 117-119.
10.	李勇, 张培建. 肝移植术后免疫排斥反应和肝功能监测 [J]. 医学研究生学报, 2008； 21(3): 313-316.
11.	易述红, 陈规划, 陆敏强, 等. VEGF mRNA在预测肝癌肝移植术后肿瘤复发和转移中的应用价值 [J]. 中国普外基础与临床杂志, 2006; 13(5): 560-564.
12.	Christou H, Yoshida A, Arthur V, et al． Increased vascular endothelial growth factor production in the lungs of rats with hypoxia-induced pulmonary hypertension [J]. Am J Respir Cell Mol Biol, 1998; 18(6): 768-776.
13.	Liu LX, Lu H, Luo Y, et al. Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1 [J]. Biochem Biophys Res Commum, 2002; 291(4): 908-914.
14.	李兴睿, 廖晓峰, 易继林. 缺氧对肝癌细胞系HepG2表达VEGF的影响 [J]. 中国普外基础与临床杂志, 2005; 12(5): 477-479.
15.	Shibuya M, Claesson-Welsh L. Signal transduction by VEGF receptors in regulation of angiogenesis and lymphangiogenesis [J]. Exp Cell Res, 2006; 312(5): 549-560.
16.	Wilkinson-Berka JL. Vasoactive factors and diabetic retinopathy: vascular endothelial growth factor, cycoloxygenase-2 and nitric oxide [J]. Curr Pharm Des, 2004; 10(27): 3331-3348.
17.	Teraoka H, Sawada T, Nishihara T, et al. Enhanced VEGF production and decreased immunogenicity induced by TGF beta1 promote liver metastasis of pancreatic cancer [J]. Br J Cancer, 2001; 85(4): 612-617.
18.	Zuo S, Ji Y, Wang J, et al. Expression and clinical implication of HIF-1alpha and VEGF-C in non-small cell lung cancer [J]. J Huazhong Univ Sci Technolog Med Sci, 2008; 28(6): 674-676.
19.	Gaudio E, Barbaro B, Alvaro D, et al. Vascular endothelial growth factor stimulates rat cholangiocyte proliferation via an autocrine mechanism [J]. Gastroenterology, 2006; 130(4): 1270-1282.
20.	Gaudio E, Barbara Bo, Alvaro D, et al. Administration of r-VEGF-A prevents hepatic artery ligation-induced bile duct damage in bile duct ligated rats [J]. Am J Physiol Gastrointest Liver Physiol, 2006; 291(2): G307-G317.
21.	Xu WH, Ye QF, Xia SS. Apoptosis and proliferation of intrahepatic bile duct after ischemia-reperfusion injury [J]. Hepatobiliary Pancreat Dis Int, 2004; 3(3): 428-432.
22.	李文岗, 胡素贤, 段伟东, 等. 完全失去动脉血供后大鼠肝门部胆管微血管数对胆管上皮细胞核增殖与ER表达影响 [J]. 中华肝胆外科杂志, 2007; 13(8): 548-550.
23.	Gaudio E, Onori P, Franchitto A, et al. Hepatic microcirculation and cholangiocyte physiopathology [J]. Ital J Anat Embryol, 2005; 110(2 Suppl 1): 71-75.
24.	Bissinger AL, Oettle H, Jahn G, et al. Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient [J]. J Med Virol, 2004; 73(1): 45-53.
25.	Beaussier M, Wendum D, Fouassier L, et al. Adaptative bile duct proliferative response in experimental bile duct ischemia [J]. J Hepatol, 2005; 42(2): 257-265.

1. Wang Y, Mao XO, Xie L, et al. Vascular endothelial growth factor overexpression delays neurodegeneration and prolongs survival in amyotrophic lateral sclerosis mice [J]. J Neurosci, 2007; 27 (2): 304-307.
2. Li LH, Guo ZJ, Yan LL, et al． Antitumor and antiangiogenic activities of anti-vascular endothelial growth factor hairpin ribozyme in human hepatocellular carcinoma cell cultures and xenografts [J]. World J Gastroenterol, 2007; 13(47): 6425-6432.
3. Stroka DM, Burkhardt T, Desbaillets I, et al. HIF-1 is expressed in normoxic tissue and displays an organ specific regulation under systemic hypoxia [J]. FASEB J, 2001; 15(13): 2445-2453.
4. 陶立德，张培建, 田明祥, 等. 低氧预适应对移植肝脏肝窦内皮细胞损伤的影响 [J]. 实用临床医药杂志, 2007; 11(2): 39-42.
5. 赵宏峰, 周杰. 大鼠自体原位肝移植胆道缺血再灌注损伤模型的建立 [J]. 第二军医大学学报, 2006； 27(4): 429-430.
6. 梁四海, 陶立德, 张培建, 等. 低氧预适应对大鼠移植肝脏功能和组织结构的影响 [J]. 江苏大学学报(医学版), 2007; 17(3): 219-221.
7. 张培建, 卜平, 金成. 低氧预适应对移植大鼠肝细胞线粒体超微结构的影响 [J]. 解剖学报, 2008; 39(6): 854-857.
8. 孔连宝, 吴晓峰, 邵立军, 等. 吻合肝动脉对大鼠部分肝移植早期损伤的影响 [J]. 中华实验外科杂志, 2006; 23(5): 543-545.
9. 彭方兴, 郑本波, 刘立新, 等. 大鼠原位肝移植动物模型制作要点 [J]. 中国普外基础与临床杂志, 2005; 12(2）: 117-119.
10. 李勇, 张培建. 肝移植术后免疫排斥反应和肝功能监测 [J]. 医学研究生学报, 2008； 21(3): 313-316.
11. 易述红, 陈规划, 陆敏强, 等. VEGF mRNA在预测肝癌肝移植术后肿瘤复发和转移中的应用价值 [J]. 中国普外基础与临床杂志, 2006; 13(5): 560-564.
12. Christou H, Yoshida A, Arthur V, et al． Increased vascular endothelial growth factor production in the lungs of rats with hypoxia-induced pulmonary hypertension [J]. Am J Respir Cell Mol Biol, 1998; 18(6): 768-776.
13. Liu LX, Lu H, Luo Y, et al. Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1 [J]. Biochem Biophys Res Commum, 2002; 291(4): 908-914.
14. 李兴睿, 廖晓峰, 易继林. 缺氧对肝癌细胞系HepG2表达VEGF的影响 [J]. 中国普外基础与临床杂志, 2005; 12(5): 477-479.
15. Shibuya M, Claesson-Welsh L. Signal transduction by VEGF receptors in regulation of angiogenesis and lymphangiogenesis [J]. Exp Cell Res, 2006; 312(5): 549-560.
16. Wilkinson-Berka JL. Vasoactive factors and diabetic retinopathy: vascular endothelial growth factor, cycoloxygenase-2 and nitric oxide [J]. Curr Pharm Des, 2004; 10(27): 3331-3348.
17. Teraoka H, Sawada T, Nishihara T, et al. Enhanced VEGF production and decreased immunogenicity induced by TGF beta1 promote liver metastasis of pancreatic cancer [J]. Br J Cancer, 2001; 85(4): 612-617.
18. Zuo S, Ji Y, Wang J, et al. Expression and clinical implication of HIF-1alpha and VEGF-C in non-small cell lung cancer [J]. J Huazhong Univ Sci Technolog Med Sci, 2008; 28(6): 674-676.
19. Gaudio E, Barbaro B, Alvaro D, et al. Vascular endothelial growth factor stimulates rat cholangiocyte proliferation via an autocrine mechanism [J]. Gastroenterology, 2006; 130(4): 1270-1282.
20. Gaudio E, Barbara Bo, Alvaro D, et al. Administration of r-VEGF-A prevents hepatic artery ligation-induced bile duct damage in bile duct ligated rats [J]. Am J Physiol Gastrointest Liver Physiol, 2006; 291(2): G307-G317.
21. Xu WH, Ye QF, Xia SS. Apoptosis and proliferation of intrahepatic bile duct after ischemia-reperfusion injury [J]. Hepatobiliary Pancreat Dis Int, 2004; 3(3): 428-432.
22. 李文岗, 胡素贤, 段伟东, 等. 完全失去动脉血供后大鼠肝门部胆管微血管数对胆管上皮细胞核增殖与ER表达影响 [J]. 中华肝胆外科杂志, 2007; 13(8): 548-550.
23. Gaudio E, Onori P, Franchitto A, et al. Hepatic microcirculation and cholangiocyte physiopathology [J]. Ital J Anat Embryol, 2005; 110(2 Suppl 1): 71-75.
24. Bissinger AL, Oettle H, Jahn G, et al. Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient [J]. J Med Virol, 2004; 73(1): 45-53.
25. Beaussier M, Wendum D, Fouassier L, et al. Adaptative bile duct proliferative response in experimental bile duct ischemia [J]. J Hepatol, 2005; 42(2): 257-265.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Role of Vascular Endothelial Growth Factor in Protection on Intrahepatic Cholangiocyte Mediated by Hypoxic Preconditioning after Liver Graft

Abstract Full text Figures/Tables Video References Cited by

Format

Content