Comparative Study of Two Methods of Drug Chemosensitivity Assay in Vitro for Tumor Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHOU Tao , HU Ya , LIAO Quan , ZHAO Yupei

Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China;

Corresponding author：

Keywords：

Collagen-gel droplet embedded-culture drug sensitivity test; Drug chemosensitivity assay; Tumor

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Objective To evaluate the utility of collagen-gel droplet embedded-culture drug sensitivity test (CD-DST) in pancreatic carcinoma cell by compared with WST-8. Methods The chemosensitivity to 5-fluorouracil (5-FU), gemzar (GEM) and oxaliplatin (OXA) of pancreatic adenocarcinoma cells SW1990, PCT-3 and ASPC-1 were tested by WST-8 and CD-DST respectively. Results In a certain living cell number range (500-10 000), there was a linear correlation (r=0.991 1, P＜0.05) between the integral optical density in CD-DST and the cell number. The inhibition ratios of three kinds of cell growth tested by CD-DST were higher than those tested by WST-8 (P＜0.05). The results of drug chemosensitivity to 5-FU， GEM and OXA detected by two methods were uniform. Conclusion The CD-DST can be used to assay the drug chemosensitivity in vitro for pancreatic carcinoma.

Citation： ZHOU Tao,HU Ya,LIAO Quan,ZHAO Yupei. Comparative Study of Two Methods of Drug Chemosensitivity Assay in Vitro for Tumor Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(11): 825-828. doi: Copy

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1. Yasuda H, Takada T, Wada K, et al. A new in-vitro drug sensitivity test (collagen-gel droplet embedded-culture drug sensitivity test) in carcinomas of pancreas and biliary tract: possible clinical utility [J]. J Hepatobiliary Pancreat Surg, 1998; 5(3)∶261.
2. 廖泉, 赵玉沛, 蔡力行, 等. 胰腺癌细胞对化疗药物敏感性的测定 [J]. 中华实验外科杂志， 1998; 15(5)∶ 444.
3. Kobayashi H, Higashiyama M, Minamigawa K, et al. Examination of in vitro chemosensitivity test using collagen gel droplet culture method with colorimetric endpoint quantification [J]. Jpn J Cancer Res, 2001; 92(2)∶203.
4. Alley MC, Scudiero DA, Monks A, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay [J]. Cancer Res, 1988; 48(3)∶589.
5. Yang J, Richards J, Bowman P, et al. Sustained growth and three-dimensional organization of primary mammary tumor epithelial cells embedded in collagen gels [J]. Proc Natl Acad Sci USA, 1979; 76(7)∶3401.
6. Richards J, Larson L, Yang J, et al. Method for culturing mammary epithelial cells in a rat tail collagen gel matrix [J]. Methods Cell Sci, 1983; 8(1)∶ 31.
7. Kawamura M, Gika M, Abiko T, et al. Clinical evaluation of chemosensitivity testing for patients with unresectable non-small cell lung cancer (NSCLC) using collagen gel droplet embedded culture drug sensitivity test (CD-DST) [J]. Cancer Chemother Pharmacol, 2007; 59(4)∶507.
8. Vaccarello L, Wang YL, Whiteside TL. Sustained outgrowth of autotumor-reactive T lymphocytes from human ovarian carcinomas in the presence of tumor necrosis factor alpha and interleukin 2 [J]. Hum Immunol, 1990; 28(2)∶216.
9. Sieuwerts AM, Klijn JG, Peters HA, et al. The MTT tetrazolium salt assay scrutinized: how to use this assay reliably to measure metabolic activity of cell cultures in vitro for the assessment of growth characteristics, IC50-values and cell survival [J]. Eur J Clin Chem Clin Biochem, 1995; 33(11)∶813.
10. Fricker SP, Buckley RG. Comparison of two colorimetric assays as cytotoxicity endpoints for an in vitro screen for antitumour agents [J]. Anticancer Res, 1996; 16(6B)∶3755.
11. Chen CF, Hwang JM, Wu CH, et al. Evaluation of a rapid tetrazolium-based colorimetric assay for selecting anticancer drugs [J]. Zhonghua Yi Xue Za Zhi (Taipei), 1990; 46(1)∶7.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Comparative Study of Two Methods of Drug Chemosensitivity Assay in Vitro for Tumor Cells

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