Effects of Immature Dendritic Cell Transfected by TGF-β1 Recombinated Adenovirus Vector on T Lymphocyte in Peripheral Blood and Allograft af ter Small Bowel Transplantation in Rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Jipeng , HUANG Yi , WANG Weizhong , DONG Guanglong , DU Jianjun , CHEN Dongli , JI Gang , LIU Ji

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Keywords：

转化生长因子β1,小肠移植,T 细胞

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目的　探讨经转化生长因子-β1 ( TGF-β1) 基因修饰的未成熟树突状细胞(imDC) 预处理大鼠小肠移
植受体后的外周血及移植肠浸润T 细胞的变化及意义。方法　选用近交系F344/ N 和BN 大鼠建立全小肠异位移
植模型,实验分4 组(每组24 只) : 同基因移植组(BN-BN 组) 、异基因移植组( F344/ N-BN 组) 、异基因移植+ TGF-
β1 基因转染imDC 组( F344/ N-BN + TGF2β1 组) 和异基因移植+ TGF-β1 基因转染imDC + FK506 组( F344/ N-
BN + TGF-β1 + FK506 组) 。各组大鼠分别于术后3 、5 、7 d 各处死6 只,获取大鼠静脉血和移植肠。应用免疫组化
SABC 法检测受体鼠外周血及移植肠CD4 + 、CD8 + 、CD25 + 细胞和IL-4 的表达。同时行移植肠组织病理学检查
并观察大鼠生存情况。结果　TGF-β1 修饰的DC 细胞能显著抑制外周血及移植肠浸润淋巴细胞CD4 + 、CD8 + 及
CD25 + 的表达,并提高IL-4 的表达; 显著延长受体大鼠的生存时间,但移植肠仍有排斥反应的病理组织学征象。
结论　TGF-β1 修饰的DC 通过影响受体外周血及移植肠浸润T 细胞对大鼠小肠移植发挥免疫抑制作用。

Citation： LI Jipeng,HUANG Yi,WANG Weizhong,DONG Guanglong,DU Jianjun,CHEN Dongli,JI Gang,LIU Ji,.,Department of Gastrointestinal Surgery,Xijing Hospital,The Fourth Military Medical University,Xian,China Corresponding Author WANG Weizhong,Email weichangfmmu.edu.cn. Effects of Immature Dendritic Cell Transfected by TGF-β1 Recombinated Adenovirus Vector on T Lymphocyte in Peripheral Blood and Allograft af ter Small Bowel Transplantation in Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(2): 96-101. doi: Copy

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13.	Krieger NR,Yin DP,Fathman CG.CD4+ but not CD8+ cells are essential for allorejection[J].J Exp Med,1996; 184(5):2013.
14.	He G,Hart J,Kim OS,et al.The role of CD8 and CD4 T cells in intestinal allograft rejection:a comparison of monoclonal antibody-treated and knockout mice[J].Transplantation,1999; 67(1):131.
15.	Takeuchi M,Alard P,Streilein JW.TGF-beta promotes immune deviation by altering accessory signals of antigen-presenting cells[J].J Immunol,1998; 160(4):1589.
16.	Rastellini C,Lu L,Ricordi C,et al.Granulocyte/macrophage colony-stimulating factor-stimulated hepatic dendritic cell progenitors prolong pancreatic islet allograft survival[J].Transplantation,1995; 60(11):1366.
17.	Itoh M,Takahashi T,Sakaguchi N,et al.Thymus and autoimmunity:production of CD25+CD4+ naturally anergic and suppressive T cells as a key function of the thymus in maintaining immunologic self-tolerance[J].J Immunol,1999; 162(9):5317.
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19.	姜楠,李立.转化生长因子β在移植排斥中的作用[J].中国普外基础与临床杂志,2003; 10(2):179.
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1. Grant D.Current results of intestinal transplantation.The International Intestinal Transplant Registry[J].Lancet,1996; 347(9018):1801.
2. Zhai Y,Kupiec-Weglinski JW.What is the role of regulatory T cells in transplantation tolerance?[J].Curr Opin Immunol,1999; 11(5):497.
3. Fairchild PJ,Waldmann H.Dendritic cells and prospects for transplantation tolerance[J].Curr Opin Immunol,2000; 12(5):528.
4. Hackstein H,Morelli AE,Thomson AW.Designer dendritic cells for tolerance induction:guided not misguided missiles[J].Trends Immunol,2001; 22(8):437.
5. Dhodapkar MV,Steinman RM,Krasovsky J,et al.Antigen-specific inhibition of effector T cell function in humans after injection of immature dendritic cells[J].J Exp Med,2001; 193(2):233.
6. 李纪鹏,黄怡,王为忠,等.TGF-1基因转染对大鼠树突状细胞生物学特性的影响[J].山东医药,2007; 47(24):23.
7. 董光龙,李开宗,王为忠,等.双Cuff双造口大鼠异位小肠移植模型的建立[J].第四军医大学学报,2003; 24(2):189.
8. Toogood GJ,Rankin AM,Tam PK,et al.The immune response following small bowel transplantation.Ⅱ.A very early cytokine response in the gut-associated lymphoid tissue[J].Transplantation,1997; 63(8):1118.
9. Kezuka T,Streilein JW.Analysis of in vivo regulatory properties of T cells activated in vitro by TGF beta 2-treated antigen presenting cells[J].Invest Ophthalmol Vis Sci,2000; 41(18):1410.
10. 赵振林,郭永章,李立,等.T细胞疫苗诱导免疫耐受与外周血T细胞凋亡的关系探讨[J].中国普外基础与临床杂志,2003; 10(4):359.
11. 谭广,任金帅,王忠裕,等.TNF-α增强树突状细胞诱导的抗胰腺癌免疫应答的实验研究[J].中国普外基础与临床杂志,2006; 13(6):689.
12. Newell KA,He G,Hart J,et al.Treatment with either anti-CD4 or anti-CD8 monoclonal antibodies blocks alphabeta T cell-mediated rejection of intestinal allografts in mice[J].Transplantation,1997; 64(7):959.
13. Krieger NR,Yin DP,Fathman CG.CD4+ but not CD8+ cells are essential for allorejection[J].J Exp Med,1996; 184(5):2013.
14. He G,Hart J,Kim OS,et al.The role of CD8 and CD4 T cells in intestinal allograft rejection:a comparison of monoclonal antibody-treated and knockout mice[J].Transplantation,1999; 67(1):131.
15. Takeuchi M,Alard P,Streilein JW.TGF-beta promotes immune deviation by altering accessory signals of antigen-presenting cells[J].J Immunol,1998; 160(4):1589.
16. Rastellini C,Lu L,Ricordi C,et al.Granulocyte/macrophage colony-stimulating factor-stimulated hepatic dendritic cell progenitors prolong pancreatic islet allograft survival[J].Transplantation,1995; 60(11):1366.
17. Itoh M,Takahashi T,Sakaguchi N,et al.Thymus and autoimmunity:production of CD25+CD4+ naturally anergic and suppressive T cells as a key function of the thymus in maintaining immunologic self-tolerance[J].J Immunol,1999; 162(9):5317.
18. Hsu FJ,Benike C,Fagnoni F,et al.Vaccination of patients with B-cell lymphoma using autologous antigen-pulsed dendritic cells[J].Nat Med,1996; 2(1):52.
19. 姜楠,李立.转化生长因子β在移植排斥中的作用[J].中国普外基础与临床杂志,2003; 10(2):179.
20. Aziz T,Hasleton P,Hann AW,et al.Transforming growth factor beta in relation to cardiac allograft vasculopathy after heart transplantation[J].J Thorac Cardiovasc Surg,2000; 119(4 Pt 1):700.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Immature Dendritic Cell Transfected by TGF-β1 Recombinated Adenovirus Vector on T Lymphocyte in Peripheral Blood and Allograft af ter Small Bowel Transplantation in Rat

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