Transplantation of Autologous Peripheral Blood Stem Cells for The Treatment of Lower Limb Arterial Ischemic Disease_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

MIAO Jianhang , SHI Tianxiong , HU Xixiang , SUN Jianming , ZHANG Mingguang

The Second Department of General Surgery, Zhongshan People’s Hospital, Guangdong Province, Zhongshan528403, China Corresponding Author： MIAO Jian-hang, E-mail： lymjh@yeah.net;

Corresponding author：

Keywords：

Stem cell,Arterial ischemic disease,Lower limb

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Objective 　To evaluate the clinical efficacy of t ransplantation of autologous peripheral blood stem
cells ( PBSC) for the t reatment of lower limb arterial ischemic disease. Methods 　From March 2004 to February
2007 , 16 patient s with severe lower limb arterial ischemic disease were t reated with autologous PBSC t ransplantation. Recombinated granulocytecolony stimulating factor ( G2CSF) was used to mobilize the proliferation of bone
marrow stem cells and then the stem cells were released into peripheral blood. Af ter 5 - 6 days , PBSC were collected by CS23000 PLUS blood2cells separator. Such concent rated stem cells fluid was int ramuscularly injected into the
ischemic areas of the lower limbs. Results 　The result s of 3 to 242month following2up after the t ransplantation of
stem cells showed that the resting pains of the affected limb were greatly relieved , and ulcers were healed. The distance and duration of intermittent limping became farther and longer. Conclusion 　Transplantation of autologous
PBSC would be a novel and effective method for the t reatment of arterial ischemic disease. However , this method is
still at the stage of initial clinical application , so it still need to be further studied.

Citation： MIAO Jianhang,SHI Tianxiong,HU Xixiang,SUN Jianming,ZHANG Mingguang,.. Transplantation of Autologous Peripheral Blood Stem Cells for The Treatment of Lower Limb Arterial Ischemic Disease. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(2): 106-108. doi: Copy

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12.	Kalka C,Masuda H,Takahashi T,et al.Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization[J].Proc Natl Acad Sci USA,2000; 97(7):3422.
13.	Shintani S,Murohara T,Ikeda H,et al.Mobilization of endothelial progenitor cells in patients with acute myocardial infarction[J].Circulation,2001; 103(23):2776.
14.	Isner JM,Asahara T.Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization[J].J Clin Invest,1999; 103(9):1231.
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16.	Reyes M,Dudek A,Jahagirdar B,et al.Origin of endothelial progenitors in human postnatal bone marrow[J].J Clin Invest,2002; 109(3):337.
17.	Alessandri G,Girelli M,Taccagni G,et al.Human vasculogenesis ex vivo:embryonal aorta as a tool for isolation of endothelial cell progenitors[J].Lab Invest,2001; 81(6):875.
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19.	Masuda H,Asahara T.Post-natal endothelial progenitor cells for neovascularization in tissue regeneration[J].Cardiovasc Res,2003; 58(2):390.

1. <><><><> [1]钱爱民,李晓强,段鹏飞,等.自体外周血干细胞移植治疗老年人慢性下肢缺血临床研究[J].南通大学学报(医学版),2007; 27(2):109.
2. 赵萍.干细胞移植治疗下肢缺血性疾病的临床进展[J].医学综述,2007; 13(10):775.
3. 张玉浩,戴坤扬.内皮祖细胞在下肢缺血性疾病治疗中的研究现状[J].临床外科杂志,2006; 14(10):668.
4. 黄蓉,夏宁,梁瑜祯.干细胞移植治疗下肢缺血性疾病的研究进展[J].中国组织工程研究与临床康复,2007; 11(7):1305.
5. 王卫东,陈正堂.血管内皮祖细胞研究进展[J].中国微循环,2002; 6(1):6.
6. 胡咏梅,罗羽慧.骨髓干细胞移植在心血管疾病中的应用[J].心血管病学进展,2003; 24(1):38.
7. 袁红丰,裴雪涛.内皮祖细胞在心脑血管疾病治疗中的应用[J].生理科学进展,2003; 34(2):165.
8. 李燕,高平进,朱鼎良.内皮祖细胞研究进展[J].生理科学进展,2002; 33(3):245.
9. Lin Y,Weisdorf DJ,Solovey A,et al.Origins of circulating endothelial cells and endothelial outgrowth from blood[J].J Clin Invest,2000; 105(1):71.
10. Bender JG,Unverzagt KL,Walker DE,et al.Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry[J].Blood,1991; 77(12):2591.
11. Asahara T,Murohara T,Sullivan A,et al.Isolation of putative progenitor endothelial cells for angiogenesis[J].Science,1997; 275(5302):964.
12. Kalka C,Masuda H,Takahashi T,et al.Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization[J].Proc Natl Acad Sci USA,2000; 97(7):3422.
13. Shintani S,Murohara T,Ikeda H,et al.Mobilization of endothelial progenitor cells in patients with acute myocardial infarction[J].Circulation,2001; 103(23):2776.
14. Isner JM,Asahara T.Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization[J].J Clin Invest,1999; 103(9):1231.
15. Poole TJ,Finkelstein EB,Cox CM.The role of FGF and VEGF in angioblast induction and migration during vascular development[J].Dev Dyn,2001; 220(1):1.
16. Reyes M,Dudek A,Jahagirdar B,et al.Origin of endothelial progenitors in human postnatal bone marrow[J].J Clin Invest,2002; 109(3):337.
17. Alessandri G,Girelli M,Taccagni G,et al.Human vasculogenesis ex vivo:embryonal aorta as a tool for isolation of endothelial cell progenitors[J].Lab Invest,2001; 81(6):875.
18. Asahara T,Isner JM.Endothelial progenitor cells for vascular regeneration[J].J Hematother Stem Cell Res,2002; 11(2):171.
19. Masuda H,Asahara T.Post-natal endothelial progenitor cells for neovascularization in tissue regeneration[J].Cardiovasc Res,2003; 58(2):390.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Transplantation of Autologous Peripheral Blood Stem Cells for The Treatment of Lower Limb Arterial Ischemic Disease

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