Objective To establish a VX2 liver tumor model in rabbits under guidance of ultrasound mini-invasively, and to evaluate the imaging characteristics of VX2 liver tumor on ultrasound, CT scanning and angiography，respectively．
Methods VX2 tumor tissues that were planted intramuscularly in the rabbit’s hind leg, were resected and cut into small pieces in 0.5 mm×0.5 mm×0.5 mm, and were inoculated into the left lobes of livers in 60 New Zealand rabbits under the guidance of ultrasound. The achievement ratio of the inoculated tumors growing in the rabbit livers were measured, and the imaging characteristics of the tumors on ultrasound, CT scanning and angiography were observed and then were evaluated 2 weeks later.
Results The achievement ratio of establishing a liver tumor model under the guidance of ultrasound was 95% （57/60）. The average physio-life span of the model rabbits was (45±8) d. Ultrasound showed that the tumor in the rabbit liver was a single spherical or sphere-alike hypoechoic nodus, and there were higher blood flow signals in and around the nodus. VX2 tumor in the liver appeared as a solitary low density nodus on unenhanced CT scanning, and the hepatic arteriography showed that the tumor was rich of blood vessels, which built the disordered vasoganglion. Tumor was maily stained at the edge of the nodus.
Conclusion It may be simple and effective to estabish a rabbit liver tumor model by inoculating VX2 tumor tissue under the guidance of ultrasound and the achievement ratio of such method was relatiely high. And the imaging characteristics of the tumor was similar to that of human primary liver carcinoma.
Citation：
YANG Guohua,CHEN Xiaoping,HUANG Zhiyong,SUN Zhengang,ZHANG Wanguang,GUAN Jian,LUO Hongchang. Establishing A VX2 Rabbit Liver Tumor Model by Injecting Tumor Tissue Under The Guidance of Ultrasound. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(3): 273-275. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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贾洪顺， 全显跃. 改进兔肝动脉插管术: 肝血管影像研究 [J]. 放射学实践, 2002; 17（4）∶324.
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Lin WY, Chen J, Lin Y, et al. Implantation of VX2 carcinoma into the liver of rabbits: a comparison of three direct-injection methods [J]. J Vet Med Sci， 2002; 64(7)∶649.
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邵国良， 周康荣， 王建华, 等. 介入治疗实验研究中兔VX2肝癌模型制作的改进和CT评价 [J]. 临床放射学杂志, 2000; 19(10)∶653.
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张盟辉， 孔宪炳， 王巧玲， 等. 射频消融联合亚砷酸局部治疗对兔肝VX2肿瘤MVD和VEGF表达的影响 [J]. 中国普外基础与临床杂志, 2007; 14（1）∶19.
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范林军， 何振平， 马宽生, 等. 兔肝VX2肿瘤移植模型的复制及其意义 [J]. 中国普外基础与临床杂志, 2004; 11（1）∶45.
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- 1. Hanazaki K, Matsushita A, Nakagawa K, et al. Risk factors of intrahepatic recurrence after curative resection of hepatocellular carcinoma [J]. Hepatogastroenterology， 2005; 52(62)∶580.
- 2. 李锦清， 张昌卿， 张亚奇， 等. 肝癌切除术后辅助肝动脉栓塞化疗的价值 [J]. 中国普外基础与临床杂志, 2006; 13（2）∶135.
- 3. Ko YH, Pedersen PL, Geschwind JF. Glucose catabolism in the rabbit VX2 tumor model for liver cancer: characterization and targeting hexokinase [J]. Cancer Lett， 2001; 173(1)∶83.
- 4. Moroz P, Jones SK, Winter J, et al. Targeting liver tumors with hyperthermia: ferromagnetic embolization in a rabbit liver tumor model [J]. J Surg Oncol， 2001; 78(1)∶22.
- 5. Matsumura T, Moriyasu F, Kono Y, et al. Contrast-enhanced power Doppler imaging of the liver-preliminary animal study [J]. Nippon Rinsho， 1998; 56(4)∶985.
- 6. Leander T, Harada T, Yuri Y, et al. CT and MR imaging of the liver using liver specific contrast media. A comparative study in a tumor model [J]. Acta Radiol， 1996； 37(3)∶649.
- 7. Kigure T, Harada T, Yuri Y, et al. Ultrasound-guided microwave thermotherapy on a VX-2 carcinoma implanted in rabbit kidney [J]. Ultrasound Med Biol， 1995; 21(5)∶649.
- 8. Chamberlain M, Gray B, Heggle S, et al. Hepatic metastasis a phyaiological approach to treatment [J]. Br J Surg， 1983； 70（9）∶596.
- 9. 贾洪顺， 全显跃. 改进兔肝动脉插管术: 肝血管影像研究 [J]. 放射学实践, 2002; 17（4）∶324.
- 10. Lin WY, Chen J, Lin Y, et al. Implantation of VX2 carcinoma into the liver of rabbits: a comparison of three direct-injection methods [J]. J Vet Med Sci， 2002; 64(7)∶649.
- 11. 邵国良， 周康荣， 王建华, 等. 介入治疗实验研究中兔VX2肝癌模型制作的改进和CT评价 [J]. 临床放射学杂志, 2000; 19(10)∶653.
- 12. 张盟辉， 孔宪炳， 王巧玲， 等. 射频消融联合亚砷酸局部治疗对兔肝VX2肿瘤MVD和VEGF表达的影响 [J]. 中国普外基础与临床杂志, 2007; 14（1）∶19.
- 13. 范林军， 何振平， 马宽生, 等. 兔肝VX2肿瘤移植模型的复制及其意义 [J]. 中国普外基础与临床杂志, 2004; 11（1）∶45.
