Effects of Endogenous Photodynamic Therapy on Intracellular cAMP and cGMP Concentrations of Human Colon Carcinoma Cell Lines SW480_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHENG Jianghua ¹ , SHI De ¹ , CHEN Zulin ² , XIAO Weidong ² , LIANG Ping. ²

Department of Vascular Surgery, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China;

Corresponding author：

Keywords：

Endogenous photodynamic therapy SW480 cell cAMP cGMP

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【Abstract】ObjectiveTo investigate the effects of endogenous photodynamic therapy (PDT) on intracellular cAMP and cGMP concentrations of human colon carcinoma cell lines SW480.
MethodsSW480 cells were divided into control group, light group, δaminolevulinic acid （ALA） group (ALA group) and endogenous PDT group (ALAPDT group). Intracellular cAMP and cGMP concentrations of each group were detected by radioimmunoassay at 30, 60, 90 and 120 min after irradiation.
ResultsThere was a significant increase in intracellular cAMP concentration of ALAPDT group at 30 min after irradiation (P＜0.001) and sequent decrease, but intracellular cAMP concentrations of ALAPDT group at 60, 90 and 120 min after irradiation had no statistical difference than the other groups (P gt;0.05). Intracellular cGMP concentration of different time point of each group was not significantly different.
ConclusionThese results indicate that the cytoprotection of SW480 cell are produced by an instantaneous increase in the intracellular cAMP concentration while endogenous PDT is killing SW480 cell.

Citation： ZHENG Jianghua,SHI De,CHEN Zulin,XIAO Weidong,LIANG Ping.. Effects of Endogenous Photodynamic Therapy on Intracellular cAMP and cGMP Concentrations of Human Colon Carcinoma Cell Lines SW480. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(3): 271-274. doi: Copy

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16.	Rossi AG, Cousin JM, Dransfield I, et al. Agents that elevate cAMP inhibit human neutrophil apoptosis [Ｊ]. Biochem Biophys Res Commun, 1995; 217(3)∶892.
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1. Eur J Cancer， 1996; 32A Suppl 5∶S23.
2. Van Cutsem E. A glimpse of the future. New directions in the treatment of colorectal cancer [Ｊ].
3. Almeida RD, Manadas BJ, Carvalho AP, et al. Intracellular signaling mechanisms in photodynamic therapy [Ｊ]. Biochim Biophys Acta， 2004; 1704(2)∶59.
4. Muhl H, Nitsch D, Sandau K, et al. Apoptosis is triggered by the cyclic AMP signalling pathway in renal mesangial cells [Ｊ]. FEBS Lett， 1996; 382(3)∶271.
5. Lomo J, Blomhoff HK, Beiske K, et al. TGFbeta 1 and cyclic AMP promote apoptosis in resting human B lymphocytes [Ｊ]. J Immunol, 1995; 154(4)∶1634.
6. Aharoni D, Dantes A, Oren M, et al. cAMPmediated signals as determinants for apoptosis in primary granulosa cells [Ｊ]. Exp Cell Res, 1995; 218(1)∶271.
7. Loweth AC, Williams GT, Scarpello JH, et al. Evidence for the involvement of cGMP and protein kinase G in nitric oxideinduced apoptosis in the pancreatic Bcell line, HITT15 [Ｊ]. FEBS Lett, 1997; 400(3)∶285.
8. Wu CF, Bishopric NH, Pratt RE. Atrial natriuretic peptide induces apoptosis in neonatal rat cardiac myocytes [Ｊ]. J Biol Chem, 1997; 272(23)∶14860.
9. Genaro AM, Hortelano S, Alvarez A, et al. Splenic B lymphocyte programmed cell death is prevented by nitric oxide release through mechanisms involving sustained Bcl2 levels [Ｊ]. J Clin Invest, 1995; 95(4)∶1884.
10. Johansen LS. Photodynamic therapy. A new method for the treatment of cancer [Ｊ]. Ugeskr Laeger, 1999; 161(26)∶3992.
11. Peng Q, Berg K, Moan J, et al. 5Aminolevulinic acidbased photodynamic therapy: principles and experimental research [Ｊ]. Photochem Photobiol, 1997; 65(2)∶235.
12. Gahlen J, Stern J, Laubach HH, et al. Improving diagnostic staging laparoscopy using intraperitoneal lavage of deltaaminolevulinic acid (ALA) for laparoscopic fluorescence diagnosis [Ｊ]. Surgery, 1999; 126(3)∶469.
13. 陈祖林，唐建民主编. 胃肠肿瘤与现代激光 [M]. 北京：军事医学科学出版社，1999∶70～80.
14. Parvathenani LK, Buescher ES, ChaconCruz E, et al. Type Ⅰ cAMPdependent protein kinase delays apoptosis in human neutrophils at a site upstream of caspase3 [Ｊ]. J Biol Chem, 1998; 273(12)∶6736.
15. Wolvetang EJ, Johnson KL, Krauer K, et al. Mitochondrial respiratory chain inhibitors induce apoptosis [Ｊ]. FEBS Lett, 1994; 339(1-2)∶40.
16. Rossi AG, Cousin JM, Dransfield I, et al. Agents that elevate cAMP inhibit human neutrophil apoptosis [Ｊ]. Biochem Biophys Res Commun, 1995; 217(3)∶892.
17. GarciaBermejo L, Perez C, Vilaboa NE, et al. cAMP increasing agents attenuate the generation of apoptosis by etoposide in promonocytic leukemia cells [Ｊ]. J Cell Sci, 1998; 111 (Pt 5)∶637.
18. Penning LC, VanSteveninck J, Dubbelman TM. HPDinduced photodynamic changes in intracellular cyclic AMP levels in human bladder transitional carcinoma cells, clone T24 [Ｊ]. Biochem Biophys Res Commun, 1993; 194(3)∶1084.
19. Inanami O, Yoshito A, Takahashi K, et al. Effects of BAPTAAM and forskolin on apoptosis and cytochrome c release in photosensitized Chinese hamster V79 cells [Ｊ]. Photochem Photobiol, 1999; 70(4)∶650.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Endogenous Photodynamic Therapy on Intracellular cAMP and cGMP Concentrations of Human Colon Carcinoma Cell Lines SW480

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