【Abstract】 Objective To study the effects of ischemic preconditioning (IP) on the activity of nuclear factor-κB （NF-κB） and the expressions of TNF-α and intercellular adhesion molecule-1 (ICAM-1) during early reperfusion following liver transplantation in rats. Methods The models of rat orthotopic liver transplantation were established. The donor livers were stored for 2 hours in Ringers solution at 4 ℃ before transplantation. All rats were randomly divided into sham operation group （SO group）， control group and IP group. IP group was achieved by clamping the portal vein and hepatic artery of donor liver for 10 minutes followed by reperfusion for 10 minutes before harvesting. The activity of NF-κB and expressions of TNF-α and ICAM-1 at 1 h, 2 h, 4 h and 6 h after reperfusion were measured. Serum ALT, LDH were also determined. Results The liver function of recipients with IP were significantly improved. Compared with SO group， the graft NF-κB activity increased after transplantation in control group and IP group (P＜0.05), while compared with control group that was significantly attenuated at 1 h and 2 h in IP group. Similarly, hepatic levels of TNF-α and ICAM-1 were significantly elevated in control group and were reduced in IP group. Conclusion IP might down-regulated TNF-α and ICAM-1 expression in the grafts after orthotopic liver transplantation through depressed NF-κB activation， and attenuate neutrophil infiltration in the grafts after reperfusion.
Citation：
MA Yuefeng,LI Xiangcheng,YAO Aihua,YOU Wei,WANG Xuehao.. Effect of Ischemic Preconditioning on Nuclear Factor-κB Activation During Early Reperfusion Following Orthotopic Liver Transplantation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 531-534. doi:
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- 1. Clavien PA, Harvey PR, Strasberg SM. Preservation and reperfusion injuries in liver allografts. An overview and synthesis of current studies [J]. Transplantation, 1992; 53(5)∶957.
- 2. 涂 兵， 严律南， 刘智敏， 等. 缺血预处理对大鼠移植肝脏缺血再灌注损伤的保护作用 [J]. 中国普外基础与临床杂志， 2002； 9（2）∶89.
- 3. Zhang SJ, Chen S. The role of ischemic preconditioning in rat liver graft [J].Transplant Proc, 2004; 36(7)∶1970.
- 4. 马跃峰， 李相成， 游 伟. “两袖套法”大鼠原位肝移植模型的技术改进 [J]. 中国现代医学杂志， 2006； 16（2）∶229.
- 5. Kataoka M, Shimizu H, Mitsuhashi N, et al. Effect of cold-ischemia time on C-X-C chemokine expression and neutrophil accumulation in the graft liver after orthotopic liver transplantation in rats [J]. Transplantation， 2002; 73(11)∶1730.
- 6. Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia-reperfusion injury [J]. Am J Surg， 2001; 181(2)∶160.
- 7. Ricciardi R, Kim RD, McDade TP, et al. NF kappa B expression during cold ischemia correlates with postreperfusion graft function [J]. J Surg Res， 2000; 93(1)∶35.
- 8. Sindram D, Rudiger HA, Upadhya AG, et al. Ischemic preconditioning protects against cold ischemic injury through an oxidative stress dependent mechanism [J]. J Hepatol， 2002; 36(1)∶78.
- 9. Nilsson B, Friman S, Wallin M, et al. The liver protective effect of ischemic preconditioning may be mediated by adenosine [J]. Transpl Int， 2000; 13 Suppl 1∶S558.
- 10. Yamamoto Y, Kume M, Yamaoka Y. Implications of heat shock proteins during liver surgery and liver perfusion [J]. Recent Results Cancer Res， 1998; 147∶157.
- 11. Jiang Y, Gu XP, Qiu YD, et al. Ischemic preconditioning decreases C-X-C chemokine expression and neutrophil accumulation early after liver transplantation in rats [J]. World J Gastroenterol， 2003; 9(9)∶2025.
- 12. Tsuyama H, Shimizu K, Yoshimoto K, et al. Protective effect of ischemic preconditioning on hepatic ischemia-reperfusion injury in mice [J]. Transplant Proc， 2000; 32(7)∶2310.
- 13. Ohkita M, Takaoka M, Shiota Y, et al. Nitric oxide inhibits endothelin-1 production through the suppression of nuclear factor kappa B [J]. Clin Sci (Lond)， 2002; 103 Suppl 48∶68S.
- 14. Rudiger HA, Clavien PA. A cytotoxic drug against reperfusion injury? [J]. Hepatology， 2000; 31(2)∶533.
