Expression and Significance of Cyclooxygenase-2 and Angiopoietin-2 in Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Ning , TAO Kaixiong , WANG Guobin , TIAN Yuan.

Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;

Corresponding author：

Keywords：

Colorectal cancerCyclooxygenase-2Angiopoietin-2

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【Abstract】　Objective　To study the expressions of cyclooxygenase-2 (Cox-2) and angiopoietin-2 (Ang-2) in colorectal cancer tissues, cancer adjacent tissues and normal colorectal tissues, and the relationship between these expressions and the clinicopathologic features of colorectal cancer. Methods　Forty-five excised samples of colorectal adenocarcinoma were confirmed pathologically and 39 of them were of well or moderately differentiated and 6 of poorly differentiated. Lymph nodes metastasis developed in 30 patients. And 15 cases were in stage of A or B and the rest were in the stage of C or D according to the Dukes stage. Taken PBS as the negative control and the verified Cox-2 or Ang-2 positive sections as positive controls, this study detected the expressions of Cox-2 and Ang-2 protein in 45 colorectal cancer tissues, 45 cancer adjacent tissues and 15 normal colorectal tissues by using immunohistochemical SP technique method. Results　Cox-2 and Ang-2 were expressed in colorectal cancer tissues and cancer adjacent tissues, but were not expressed in normal colorectal tissues. In 45 colorectal cancer tissues, the positive expression rates of Cox-2 and Ang-2 were 80.0% and 66.7%; in 45 cancer adjacent tissues, the positive expression rates of Cox-2 and Ang-2 were 35.6% and 11.1%, respectively. The positive expression rates of both Cox-2 and Ang-2 in colorectal cancer tissues were significantly higher than those in cancer adjacent and normal colorectal tissues. There were close correlations between the expressions of Cox-2 and Ang-2 and some pathologic features, such as lymph node metastasis and Dukes stage; whereas there were no significant association between the expressions and gender, histological type and position of tumor. There was also a close correlation between the expressions of Cox-2 and Ang-2 themselves. Conclusion　Cox-2 and Ang-2 play an important role in the occurrence and development of colorectal cancer. The use of specific inhibitor of Cox-2 as a treatment for colorectal cancer may become feasible and necessary.

Citation： ZHANG Ning,TAO Kaixiong,WANG Guobin,TIAN Yuan.. Expression and Significance of Cyclooxygenase-2 and Angiopoietin-2 in Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2006, 13(5): 564-567. doi: Copy

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1. 　Ozdemir E, Kakehi Y, Okuno H, et al. Role of matrix metalloproteinase-9 in the basement membrane destruction of superficial urothelial carcinomas [J]. J Urol, 1999; 161(4)∶1359.
2. 　Oshima M, Dinchuk JE, Kargman SL, et al. Suppression of intestinal polyposis in Apc delta716 knockout mice by inhibition of cyclooxygenase 2 (COX-2) [J]. Cell， 1996; 87(5)∶803.
3. 　Seno H, Oshima M, Ishikawa TO, et al. Cyclooxygenase 2-and prostaglandin E(2) receptor EP(2)-dependent angiogenesis in Apc(Delta716) mouse intestinal polyps [J]. Cancer Res, 2002; 62(2)∶506.
4. 　Yancopoulos GD, Davis S, Gale NW, et al. Vascular-specific growth factors and blood vessel formation [J]. Nature, 2000; 407(6801)∶242.
5. 　Smith TJ. Cyclooxygenases as the principal targets for the actions of NSAIDs [J]. Rheum Dis Clin North Am, 1998; 24(3)∶501.
6. 　Sinicrope FA, Gill S. Role of cyclooxygenase-2 in colorectal cancer [J]. Cancer Metastasis Rev, 2004; 23(1-2)∶63.
7. 　 Ahmad SA, Liu W, Jung YD, et al. Differential expression of angiopoietin-1 and angiopoietin-2 in colon carcinoma. A possible mechanism for the initiation of angiogenesis [J]. Cancer, 2001; 92(5)∶1138.
8. 　Tsujii M, Kawano S, Tsuji S, et al.Cyclooxygenase regulates angiogenesis induced by colon cancer cells [J]. Cell, 1998; 93(5)∶705.
9. 　Williams CS, Tsujii M, Reese J, et al.Host cyclooxygenase-2 modulates carcinoma growth [J]. J Clin Invest, 2000; 105(11)∶1511.
10. 　Papapetropoulos A, Fulton D, Mahboubi K, et al. Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/survivin pathway [J]. J Biol Chem, 2000; 275(13)∶9102.
11. 　Witzenbichler B, Maisonpierre PC, Jones P, et al. Chemotactic properties of angiopoietin-1 and-2, ligands for the endothelial-specific receptor tyrosine kinase Tie2 [J]. J Biol Chem， 1998; 273(29)∶18514.
12. 　Thurston G, Suri C, Smith K, et al. Leakage-resistant blood vessels in mice transgenically overexpressing angiopoietin-1 [J]. Science， 1999; 286(5449)∶2511.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression and Significance of Cyclooxygenase-2 and Angiopoietin-2 in Colorectal Cancer

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