【Abstract】Objective To study the effects of exogenous hyaluronidase on invasive and angiogenic potential of human breast cancer cell line ZR-75-30.
MethodsThere were two groups in the study: the study group (hyaluronidase group) and the control group. The invasive potential and the angiogenic potential of human breast cancer cell ZR-75-30 were detected by the invasive model in vitro and technique of double-chamber co-culture that human breast cancer cell line ZR-75-30 and human umbilicus vein endothelium cell ECV-304 were co-cultured.
ResultsThe penetrating number of tumor cell in the study group (70.625±11.64) was significantly higher than that in the control group (22.125±6.09),P＜0.01. The tube number from ECV-304 cell induced by ZR-75-30 cell in the study group (34.5±2.4) was significantly higher than that in the control group (8.5±1.5)， P＜0.01.
ConclusionExogenous hyaluronidase can reinforth the invasive and angiogenic ability of breast cancer cells.
Citation：
WANG Liang,REN Guosheng,TU Gang,SHI Yuan.. Effects of Exogenous Hyaluronidase on Invasive and Angiogenic Capacity of Human Breast Cancer Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(3): 216-218. doi:
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- 2. Delpech B, Laquerriere A, Maingonnat C, et al. Hyaluronidase is more elevated in human brain metastases than in primary brain tumours [Ｊ]. Anticancer Res, 2002; 22(4)∶2423.
- 3. Novak U, Stylli SS, Kaye AH, et al. Hyaluronidase2 overexpression accelerates intracerebral but not subcutaneous tumor formation of murine astrocytoma cells [Ｊ]. Cancer Res, 1999; 59(24)∶6246.
- 4. Rahmanian M, Heldin P. Testicular hyaluronidase induces tubular structures of endothelial cells grown in threedimensional collagen gel through a CD44mediated mechanism [Ｊ]. Int J Cancer, 2002; 97(5)∶601.
- 5. Goshen R, Ariel I, Shuster S, et al. Hyaluronan, CD44 and its variant exons in human trophoblast invasion and placental angiogenesis [Ｊ]. Mol Hum Reprod, 1996; 2(9)∶685.
