Objective To investigate the effect of the drug-resistance characteristic of neoplasm cell on the expression of Fas during the chemical medi-cure.
Methods The adriamycin-resistance hepatic carcinoma cells (HepG2 cell lines) were estabilished by cell biology. Changes of expression of the HepG2 cell lines was determined by immunohistochemistry.
Results When the HepG2 cell lines were not induced by adriamycin, the expression of Fas of them was weak and Fas mainly existed in cell membrane. When induced by adriamycin, the expression was enhanced and Fas mainly existed in cytochylema. Simultaneously, the death rate of the cell lines changed. The death rate of the drug-resistance cell lines in 0.1 μg/ml ADM was almost as same as that of non-drug-resistance cell lines without ADM (Ｐ＞0.05) and was significantly different from that of non-drug-resistance cell lines in 0.1 μg/ml ADM (Ｐ＜0.05). Conclusion Changes of the expression of Fas may be one of the drug-resistance mechanisms of carcinoma cell.
Citation：
XIANG Liangguang,LIN Xinlong,LI Weiqing,et al.. CHANGES OF EXPRESSION Fas OF THE ADRIAMYCIN-RESISTANCE HEPATIC CARCINOMA CELLS (HepG2 CELL LINES). CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(4): 229-230. doi:
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- 1. 郑永康， 贲昆龙. 测定细胞存在与增殖的MTT法的建立 〔Ｊ〕. 免疫学杂志， 1992； 8(4)∶266.
- 2. 曹广文， 杜平. 现代癌症生物学治疗学 〔M〕. 北京： 人民军医出版社， 1995∶147～153.
- 3. Zhang XD. An electron microscopic study of apoptosis induced by adriamycin in human hepatocellular carcinoma cells of Hcc9204 line 〔Ｊ〕. Hepatol, 1995; 23(Suppl)∶231.
- 4. Itoh N. A novel protein domain required for apoptosis: mutational analysis of human Fas antigen 〔Ｊ〕. Biol Chem, 1993; 268(5)∶10932.
- 5. Tartaglia A, Ayres TM, Wong GH, et al. A novel domain within the 55 kd TNF receptor signals cell death 〔Ｊ〕. Cell, 1993; 74(9)∶845.
- 6. Suda T, Takahashi T, Golstein P, et al. Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family 〔Ｊ〕. Cell, 1993; 75(12)∶1169.
- 7. Wong GHW. Fas antigen and p55 TNF receptor signal apoptosis through distinct pathways 〔Ｊ〕. Immunol, 1994; 152(10)∶1751.
- 8. Nagata S, Golstein P. The Fas death factor 〔Ｊ〕. Science, 1995; 267(5203)∶1449.
- 9. Yans H. Expression of Fas and antiFasmediated apoptosis in human hepatocellular carcinoma cell line 〔Ｊ〕. Hepatol, 1996; 25(4)∶454.
- 10. Ouir R. Fas antigenmediated apopatsis of ovarian surface epithelial cells 〔Ｊ〕. Endocrinology, 1997; 138(11)∶4558.
