ObjectiveTo study the effects of newcastle disease virus (NDV) on tumor infiltrating lymphocyte (TIL). MethodsTILs were dissociated from 6 cases of primary hepatic carcinoma. Each case was divided into 3 portions equally, and was added 20 μl NDV with HA titer of 1∶160 (NDV group), rIL2 with the end concentration of 1 000 u/ml (rIL2 group) and 0.9% sodium chloride (control group) respectively, then cultured in the condition of 37℃，5% CO2. CD3, CD4 and CD8 were assayed by flow cytometry. The contents of IL2, IFNγ and TNFβ in supernate of NDV group and control group were examined by ELISA.ResultsCoupled material revealed that CD3 and CD4 were significantly higher in NDV group than in the other two groups (P＜0.05, P＜0.01). CD8 had no significant changes in three groups. The contents of IL2, IFNγ and TNFβ in NDV group were significantly higher than those in control group (P＜0.01, P＜0.01 and P＜0.05 respectively). ConclusionNDV can activate TIL directly.
Citation：
ZHAO Tiejun,CHEN Tao,TIAN Fuzhou,et al.. The Effects of Newcastle Disease Virus on Tumor Infiltrating Lymphocyte. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(5): 329-331. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. 赵铁军，田伏洲，尹志良，等.新城疫病毒治疗肝癌10例 [J].世界华人消化杂志，1999； 7(6)∶ 718.
- 2. Schirrmacher V, Haas C, Bonifer R, et al. Viros potentiation of tumor vaccine Tcell stimulatory capacity requires cell surface binding but not infection [J]. Clin Cancer Res, 1997; 3(7)∶1135.
- 3. Haas C, Ertel C, Gerhards R, et al. Introduction of adhesive and costimulatory immune functions into tumor cells by infection with Newcastle Disease Virus [J]. Int J Oncol,1998; 13(6)∶1105.
- 4. Kenney S，Pagano JS. Viruses as oncolytic agents: a new age for “therapeutic” viruses？ [J]. J Natl Cancer Inst, 1994; 86(16)∶1185.
- 5. Yeow WS, Beilharz MW，Lai CM. The in vitro expression patterns of individual type I interferon genes in Newcastle disease virus infected murine splenocytes and fibroblasts [J]. Int J Biochem Cell Biol,1997; 29(3)∶513.
- 6. Weidmann E, Trucco M， Whiteside TL. Relevance of the T cell receptor for immunotherapy of cancer [J]. Cancer Immunol Immunother, 1994; 39(1)∶1.
- 7. Kagi D, Seiler P, Pavlovic J, et al. The roles of perforin and Fasdependent cytotoxicity in protection against cytopathic and noncytopathic viruses [J]. Eur J Immunol, 1995; 25(10)∶3256.
- 8. Bradley LM, Yoshimoto K，Swain SL. The cytokines IL4, IFNgamma, and IL12 regulate the development of subsets of memory effector helper T cells in vitro [J]. J Immunol, 1995; 155(4)∶1713.
- 9. Noble A, Macary PA，Kemeny DM. IFNgamma and IL4 regulate the growth and differentiation of CD8+ T cells into subpopulations with distinct cytokine profiles [J]. J Immunol, 1995; 155(6)∶2928.
