Expressions and Significance of miR-196b mRNA and HoxB8 mRNA in Human Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LAN Hongbo ¹ , PAN Jie ² ,  LU Xingrong ¹ , CHI Pan. ¹

1.Department of General Surgery，Affiliated Union Hospital of Fujian Medical University，Fuzhou 350001，Fujian Province，China;;
2. Department of Emergency Surgery，Affiliated Union Hospital of Fujian Medical University，Fuzhou 350001，Fujian Province，China;

Corresponding author：

LU Xingrong, Email: lynxlxr18@163.com.cn

Keywords：

Colorectal cancer; miR-196b; HoxB8; Real-time PCR; Western blot

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Objective 　To research the expressions of miR-196b and HoxB8 mRNA in colorectal cancer and their
correlation with clinicopathologic features，and to explore the relationship between miR-196b and HoxB8 in vivo.
Methods 　Expressions of RNA （including miR-196b and HoxB8 mRNA） and HoxB8 protein were detected respectively by using quantitative real-time reverse transcriptase PCR and Western blot in 30 cases of colorectal cancer and corresponding normal
mucous membrane tissues.
Results 　In colorectal cancer tissues，expressions of miR-196b and HoxB8 mRNA were higher than those of the corresponding normal mucous membrane tissues （P＜0.05）. Expression of miR196b mRNA was assoc-iated with lymph node metastasis，neoplasm stages （Ⅰ＋ⅡandⅢ＋Ⅳ），and distant metastasis （P＜0.05），on the other
hand，no significant differences were observed regarding tumor site，size，gross type，depth of invasion，tissue differentiation，
age，and sex （P＞0.05）. Expression of HoxB8 mRNA was no significant differences concerning lymph node metastasis，
tumor stages （Ⅰ＋Ⅱ，Ⅲ＋Ⅳ），distant metastasis，tumor site，size，gross type，depth of invasion，tissue differentiation，age，and sex （P＞0.05）. The expression of miR-196b mRNA was negatively correlated with HoxB8 mRNA expression （r=-0.458，P＜0.05），and HoxB8 protein expression with no obvious correlation （r=-0.236，P＞0.05） in colorectal cancer tissues.
Conclusions 　The expressions of miR-196b and HoxB8 mRNA in colorectal cancer tissues are higher，the high expression of miR-196b mRNA is related to the tumorigenesis and progression of colorectal cancer as well as correlated with prognosis in colorectal cancer. The miR-196b inhibits the expression of HoxB8 mRNA by binding to the
3′-UTR of target HoxB8 mRNA.

Citation： LAN Hongbo,PAN Jie,LU Xingrong,CHI Pan.. Expressions and Significance of miR-196b mRNA and HoxB8 mRNA in Human Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(4): 400-405. doi: Copy

1.	Jemal A， Siegel R， Xu J， et al. Cancer statistics， 2010[J]. CA Cancer J Clin， 2010， 60(5)：277-300.
2.	卫生部医政司. 结直肠癌诊疗规范专家工作组. 结直肠癌诊疗规范(2010年版)[J]. 中华胃肠外科杂志， 2010， 13(11)： 865-875.
3.	March HN， Rust AG， Wright NA， et al. Insertional mutagenesis identifies multiple networks of cooperating genes driving intestinaltumorigenesis[J]. Nat Genet， 2011， 43 (12)：1202-1209.
4.	Velho S， Haigis KM. Regulation of homeostasis and oncogenesis in the intestinal epithelium by ras[J]. Exp Cell Res， 2011， 317(19)： 2732-2739.
5.	Hatano Y， Yamada Y， Hata K， et al. Genetic ablation of a candidatetumor suppressor gene， Rest， does not promote mouse colon carcino-genesis[J]. Cancer Sci， 2011， 102(9)： 1659-1664.
6.	Braig S， Mueller DW， Rothhammer T， et al. MicroRNA miR-196a is a central regulator of HOX-B7 and BMP4 expression in malignant melanoma[J]. Cell Mol Life Sci， 2010， 67(20)：3535-3548.
7.	Li Y， Zhang M， Chen H， et al. Ratio of miR-196s to HOXC8 messenger RNA correlates with breast cancer cell migration and metastasis[J]. Cancer Res， 2010， 70(20)：7894-7904.
8.	Edge SB， Compton CC. The American Joint Committee on Cancer：the 7th edition of the AJCC cancer staging manual and the future of TNM[J]. Ann Surg Oncol， 2010， 17(6)：1471-1474.
9.	Urbich C， Kuehbacher A， Dimmeler S. Role of microRNAs in vascular diseases， inflammation， and angiogenesis[J]. Cardiovasc Res， 2008， 79(4)：581-588.
10.	Blenkiron C， Miska EA. miRNAs in cancer：approaches， aetiology，diagnostics and therapy[J]. Hum Mol Genet， 2007， 16 Spec No 1：R106-R113.
11.	Liao YL， Hu LY， Tsai KW， et al. Transcriptional regulation of miR-196b by ETS2 in gastric cancer cells[J]. Carcinogenesis， 2012， 33(4)：760-769.
12.	Tsai KW， Hu LY， Wu CW， et al. Epigenetic regulation of miR-196b expression in gastric cancer[J]. Genes Chromosomes Cancer，.
13.	Guan Y， Mizoguchi M， Yoshimoto K， et al. MiRNA-196 is upregulated in glioblastoma but not in anaplastic astrocytoma and has prognostic significance[J]. Clin Cancer Res， 2010， 16(16)：4289-4297.
14.	Ma R， Yan W， Zhang G， et al. Upregulation of miR-196b confersa poor progno- sis inglioblastoma patients via inducing a proliferative phenotype[J]. PLoS One， 2012， 7(6)：e38096.
15.	Schimanski CC， Frerichs K， Rahman F， et al. High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells[J]. World J Gastroenterol， 2009， 15(17)：2089-2096.
16.	Benson AB 3rd， Bekaii-Saab T， Chan E， et al. Metastatic colon cancer， version 3.2013： featured updates to the NCCN guidelines[J]. J Natl Compr Canc Netw， 2013， 11(2)： 141-152.
17.	Yue Y， Farcas R， Thiel G， et al. De novo t (12；17) (p13.3；q21.3) translocation with a breakpoint near the 5′ end of the HOXB gene cluster in a patient with developmental delay and skeletal malformations[J]. Eur J Hum Genet， 2007， 15(5)：570-577.
18.	Rawat VP， Thoene S， Naidu VM， et al. Overexpression of CDX2 perturbs HOX gene expression in murine progenitors depen-ding on its N-terminal domain and is closely correlated with dereg-ulated HOX gene expression in human acute myeloid leukemia[J]. Blood， 2008， 111(1)：309-319.
19.	Takahashi O， Hamada J， Abe M， et al. Dysregulated expression of HOX and ParaHOX genes in human esophageal squamous cell carcinoma[J]. Oncol Rep， 2007， 17(4)：753-760.
20.	Vider BZ， Zimber A， Hirsch D， et al. Human colorectal carcinogenesis is associated with deregulation of homeobox gene expression[J]. Biochem Biophys Res Commun， 1997， 232(3)：742-748.
21.	Vider BZ， Zimber A， Chastre E， et al. Deregulated expression of homeobox-containing genes， HOXB6， B8， C8， C9， and Cdx-1，in human colon cancer cell lines[J]. Biochem Biophys Res Commun， 2000， 272(2)：513-518.
22.	Kawasaki H， Taira K. MicroRNA-196 inhibits HOXB8 expression in myeloid differentiation of HL60 cells[J]. Nucleic Acids Symp Ser (Oxf)， 2004， (48)：211-212.
23.	Akao Y， Nakagawa Y， Naoe T. let-7 microRNA functions as a potential growth suppressor in human colon cancer cells[J]. Biol Pharm Bull， 2006， 29(5)：903-906.
24.	Lagos-Quintana M， Rauhut R， Lendeckel W， et al. Identificationof novel genes coding for small expressed RNAs[J]. Science， 2001， 294(5543)：853-858.
25.	Reinhart BJ， Weinstein EG， Rhoades MW， et al. MicroRNAs in plants[J]. Genes Dev， 2002， 16(13)：1616-1626.
26.	， 49(11)：969-980.

1. Jemal A， Siegel R， Xu J， et al. Cancer statistics， 2010[J]. CA Cancer J Clin， 2010， 60(5)：277-300.
2. 卫生部医政司. 结直肠癌诊疗规范专家工作组. 结直肠癌诊疗规范(2010年版)[J]. 中华胃肠外科杂志， 2010， 13(11)： 865-875.
3. March HN， Rust AG， Wright NA， et al. Insertional mutagenesis identifies multiple networks of cooperating genes driving intestinaltumorigenesis[J]. Nat Genet， 2011， 43 (12)：1202-1209.
4. Velho S， Haigis KM. Regulation of homeostasis and oncogenesis in the intestinal epithelium by ras[J]. Exp Cell Res， 2011， 317(19)： 2732-2739.
5. Hatano Y， Yamada Y， Hata K， et al. Genetic ablation of a candidatetumor suppressor gene， Rest， does not promote mouse colon carcino-genesis[J]. Cancer Sci， 2011， 102(9)： 1659-1664.
6. Braig S， Mueller DW， Rothhammer T， et al. MicroRNA miR-196a is a central regulator of HOX-B7 and BMP4 expression in malignant melanoma[J]. Cell Mol Life Sci， 2010， 67(20)：3535-3548.
7. Li Y， Zhang M， Chen H， et al. Ratio of miR-196s to HOXC8 messenger RNA correlates with breast cancer cell migration and metastasis[J]. Cancer Res， 2010， 70(20)：7894-7904.
8. Edge SB， Compton CC. The American Joint Committee on Cancer：the 7th edition of the AJCC cancer staging manual and the future of TNM[J]. Ann Surg Oncol， 2010， 17(6)：1471-1474.
9. Urbich C， Kuehbacher A， Dimmeler S. Role of microRNAs in vascular diseases， inflammation， and angiogenesis[J]. Cardiovasc Res， 2008， 79(4)：581-588.
10. Blenkiron C， Miska EA. miRNAs in cancer：approaches， aetiology，diagnostics and therapy[J]. Hum Mol Genet， 2007， 16 Spec No 1：R106-R113.
11. Liao YL， Hu LY， Tsai KW， et al. Transcriptional regulation of miR-196b by ETS2 in gastric cancer cells[J]. Carcinogenesis， 2012， 33(4)：760-769.
12. Tsai KW， Hu LY， Wu CW， et al. Epigenetic regulation of miR-196b expression in gastric cancer[J]. Genes Chromosomes Cancer，.
13. Guan Y， Mizoguchi M， Yoshimoto K， et al. MiRNA-196 is upregulated in glioblastoma but not in anaplastic astrocytoma and has prognostic significance[J]. Clin Cancer Res， 2010， 16(16)：4289-4297.
14. Ma R， Yan W， Zhang G， et al. Upregulation of miR-196b confersa poor progno- sis inglioblastoma patients via inducing a proliferative phenotype[J]. PLoS One， 2012， 7(6)：e38096.
15. Schimanski CC， Frerichs K， Rahman F， et al. High miR-196a levels promote the oncogenic phenotype of colorectal cancer cells[J]. World J Gastroenterol， 2009， 15(17)：2089-2096.
16. Benson AB 3rd， Bekaii-Saab T， Chan E， et al. Metastatic colon cancer， version 3.2013： featured updates to the NCCN guidelines[J]. J Natl Compr Canc Netw， 2013， 11(2)： 141-152.
17. Yue Y， Farcas R， Thiel G， et al. De novo t (12；17) (p13.3；q21.3) translocation with a breakpoint near the 5′ end of the HOXB gene cluster in a patient with developmental delay and skeletal malformations[J]. Eur J Hum Genet， 2007， 15(5)：570-577.
18. Rawat VP， Thoene S， Naidu VM， et al. Overexpression of CDX2 perturbs HOX gene expression in murine progenitors depen-ding on its N-terminal domain and is closely correlated with dereg-ulated HOX gene expression in human acute myeloid leukemia[J]. Blood， 2008， 111(1)：309-319.
19. Takahashi O， Hamada J， Abe M， et al. Dysregulated expression of HOX and ParaHOX genes in human esophageal squamous cell carcinoma[J]. Oncol Rep， 2007， 17(4)：753-760.
20. Vider BZ， Zimber A， Hirsch D， et al. Human colorectal carcinogenesis is associated with deregulation of homeobox gene expression[J]. Biochem Biophys Res Commun， 1997， 232(3)：742-748.
21. Vider BZ， Zimber A， Chastre E， et al. Deregulated expression of homeobox-containing genes， HOXB6， B8， C8， C9， and Cdx-1，in human colon cancer cell lines[J]. Biochem Biophys Res Commun， 2000， 272(2)：513-518.
22. Kawasaki H， Taira K. MicroRNA-196 inhibits HOXB8 expression in myeloid differentiation of HL60 cells[J]. Nucleic Acids Symp Ser (Oxf)， 2004， (48)：211-212.
23. Akao Y， Nakagawa Y， Naoe T. let-7 microRNA functions as a potential growth suppressor in human colon cancer cells[J]. Biol Pharm Bull， 2006， 29(5)：903-906.
24. Lagos-Quintana M， Rauhut R， Lendeckel W， et al. Identificationof novel genes coding for small expressed RNAs[J]. Science， 2001， 294(5543)：853-858.
25. Reinhart BJ， Weinstein EG， Rhoades MW， et al. MicroRNAs in plants[J]. Genes Dev， 2002， 16(13)：1616-1626.
26. ， 49(11)：969-980.

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Expressions and Significance of miR-196b mRNA and HoxB8 mRNA in Human Colorectal Cancer

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