Purpose To detect whether a 3243 point mutation existed in age-related macular degeneration (AMD). MethodsTwenty-six cases of wet form AMD patients, ten cases of dry form AMD patients were selected,and compared with twenty nomal controls. After collecting anti-coagulated blood samples, total cellular DNA were extracted and purified. Using polymerase chain reaction and restriction fragment long polymorphism techniques, the mtDNA Ararr;G point mutation at position 3243 were detected. Results After cleaveded by restriction endonuclease Apa I, a 294 bp fragment remained only in all detected DNA samples including twenty-six wet form AMD, and ten dry form AMD. No any other fragment appeared. The result showed that there was no Ararr;G mutation at position 3243 found in AMD. Conclusion It is suggested that mtDNA 3243 point mutation due to maternal inheritance might be not concerned with both wet form AMD and dry form AMD. (Chin J Ocul Fundus Dis,2000,16:231-232)
目的 探讨复合消化酶胶囊治疗胆囊切除术后消化功能减退症的临床疗效。方法 将84例胆囊切除术后出现消化功能减退的患者按住院日单双号原则分为对照组（n=41），给予抑酸药奥美拉唑肠溶胶囊与促胃肠道动力药多潘立酮片；观察组（n=43）加用复合消化酶胶囊。比较2组患者的临床疗效。结果 观察组显效14例，有效21例，好转7例，无效1例，总有效率为97.7%（42/43）；对照组显效10例，有效15例，好转10例，无效6例，总有效率为85.4%（35/41）。观察组的临床疗效较优（P＜0.05）。用药过程中均未发生不良反应。结论 复合消化酶胶囊对胆囊切除术后因胆汁分泌障碍而引起的消化功能减退症具有确切疗效。
目的探讨腹腔镜与开腹胆囊切除术对患者肝功能及免疫功能的影响。 方法根据手术方式将84例胆囊良性病变患者分为腹腔镜胆囊切除术组（LC组，50例）及开腹胆囊切除术组（OC组，34例），比较2组患者手术前后肝功能及免疫功能指标的变化。 结果2组患者肝功能在手术前后不同时间点的差异均无统计学意义（P＞0.05）；LC组患者术后免疫功能各指标与术前比较无明显变化（P＞0.05），OC组CD3+、CD4+及CD4+/CD8+在术后1 d及3 d均较术前明显降低（P＜0.05），术后7 d恢复至术前水平（P＞0.05）。 结论LC术可引起患者术后肝功能短暂异常，但对免疫功能无明显影响，可作为胆囊切除的首选术式。
目的总结残胃癌的临床特点、治疗及预后情况。 方法回顾性分析笔者所在医院科室2003年1月至2008年1月期间收治的60例残胃癌患者的临床资料。 结果60例残胃癌发生于远端胃大部分切除＋BillrothⅡ式重建后48例（80.0%），发生于远端胃大部分切除＋BillrothⅠ式重建后9例（15.0%），发生于近端胃大部分切除术后3例（5.0%）。42例患者行根治性切除，其术后1、3及5年生存率分别为90.5%、57.1%及13.3%；18例行姑息性手术治疗，其术后1、3及5年生存率分别为16.7%、0及0。根治组患者的中位生存时间为36.7个月，姑息组为7.2个月，根治组的生存情况较好（χ2=76.98，P＜0.001）。 结论残胃癌不易早期诊断，对于高发人群需定期复查胃镜，积极的外科治疗可以提高患者的生存率及生活质量。
目的探讨腹腔镜联合胆道镜治疗胆囊及胆总管结石的临床疗效。 方法回顾性分析2010年1月至2013年1月期间收治于笔者所在科室的96例胆囊及胆总管结石患者的临床资料，根据手术方式分为开腹组、腹腔镜联合胆道镜＋T管引流术组和腹腔镜联合胆道镜＋放置鼻胆管一期缝合组3组。对3组患者的手术时间、术中出血量、肛门排气时间、住院时间、残石率、复发率、并发症发生率、手术有效率及术后淀粉酶和肝功能指标水平进行对比分析。 结果微创手术组与开腹组比较，患者的出血量、肛门排气时间、住院时间、残石率、复发率及并发症发生率均减少、缩短或降低，同时手术的有效率提高（P＜0.05）；腹腔镜联合胆道镜＋放置鼻胆管一期缝合组术后淀粉酶和肝功能指标的水平较其他2组明显降低（P＜0.05）。 结论微创手术创伤小，残石率及复发率低，并发症少，患者术后恢复快，是一种安全有效的治疗方式，值得临床推广应用。
ObjectivesTo systematically review the prognostic value of plasma soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) level in predicting 28-day mortality in sepsis.MethodsPubMed, The Cochrane Library, EMbase, Web of Science, CBM, CNKI, VIP and WanFang Data databases were electronically searched to collect studies about the prognostic value of plasma sTREM-1 in early 28-day mortality in sepsis from inception to April 16th, 2018. Two reviewers independently screened literature, extracted data and assessed the risk of bias of included studies, then, meta-analysis was performed by using Stata 14.0 software.ResultsA total of 13 studies involving 1 115 patients were included. The results of meta-analysis showed that the sensitivity and specificity were 79% and 77%, respectively. The positive likelihood ratio and the negative likelihood ratio were 3.4 and 0.28, respectively. The diagnostic ratio was 12. The overall area under the summary receiver operator characteristic (SROC) curve was 0.80.ConclusionsCurrent evidence shows that plasma sTREM-1, as a single index, may play a prognostic role in the early 28-day mortality of sepsis in patients. Due to limited quality and quantity of the included studies, more high quality studies are needed to verify the above conclusion.