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真性小眼并发葡萄膜渗漏一例

Regulating effect of Notch-1 on retinal progenitor cells differentiating into retinal ganglion cells

Objective To investigate the regulating effect of Notch-1 on retinal progenitor cells (RPC) differentiating into retinal ganglion cells (RGC). Methods RPC of 14-day embryonic SD rats were induced and differentiated in the culture medium with Notch-1 antisense oligonucleotides (experimental group) or missense oligonucleotides (control group) for 14 days. The condition of growth and differentiation of the cells were observed daily under the phase-contrast microscope. RGC were identified by Thy1.1 immunocytochemistry methods and the cellular number was counted. Results RPC in both of the two groups differentiated into various retinal cells, including Thy1.1-positive RGC. The percentage of RGC derived from RPC was 31.19%plusmn;6.90% in experimental group and 16.57%plusmn;4.31% in control group, and the difference was statistically significant (t=9.84，Plt;0.001). Conclusion Notch-1 may down-regulate the differentiation of RPC, and the inhibition of Notch-1 may promote RPC differentiating into RGC. (Chin J Ocul Fundus Dis, 2007, 23: 101-103)

生理盐水持续冲洗在封闭式负压引流患者中的护理效果分析

目的　探讨持续生理盐水冲洗在烧伤整形科封闭式负压引流（VSD）患者中的应用效果。 方法　将2012年1月－2013年5月共收集安置VSD装置的65例患者分为对照组与观察组，对照组35例，采用常规持续负压吸引的方法；观察组30例，在常规方法的基础上持续予生理盐水缓慢滴注，冲洗时间为7 d。对两组患者的堵管、漏气、创面修复及术前准备的时间情况进行比较，运用χ2检验与t检验进行统计学分析。 结果　观察组的堵管率、漏气率及术前准备的时间明显小于对照组，创面的修复疗效明显优于对照组，两组差异有统计学意义（Z=−2.241，P＜0.05）。 结论　持续VSD生理盐水冲洗，可以降低堵管及漏气，减少换药的频率，保持创面的清洁，促进肉芽组织生长，尽早的进行自体皮瓣移植的手术，从而缩短术前准备的时间，减轻了患者的痛苦，促进了病床的周转率。

LINC00636 regulates the proliferation and migration of non-small cell lung cancer cells by targeting NM23-H1

ObjectiveTo explore the mechanism by which LINC00636 promotes the proliferation and metastasis of non-small cell lung cancer (NSCLC) by targeting NM23-H1. MethodsBased on the Affymetrix® GeneChip Human Transcriptome Array 2.0 (HTA2.0), differentially expressed long non-coding RNA (lncRNA) in three pairs of NSCLC tissues with and without lymph node metastasis were analyzed, and LINC00636 was selected as the candidate gene. The effects of LINC00636 knockdown and overexpression on the proliferation, apoptosis, migration and subcutaneous tumorigenicity of PC9 cells were analyzed. The dual-luciferase assay was used to analyze the targeting regulation of LINC00636 on NM23-H1. ResultsThe expression of LINC00636 in the serum and tissues of NSCLC patients with lymph node metastasis was higher than that in patients without lymph node metastasis. LINC00636 knockdown could inhibit the invasion and proliferation of NSCLC cells. LINC00636 could target and regulate the expression of NM23-H1, and the expression of LINC00636 and NM23-H1 in NSCLC tissues and cells was negatively correlated. The inhibitory effect of NM23-H1 on the proliferation and invasion of PC9 cells could be partially reversed by LINC00636 overexpression. ConclusionLINC00636 promotes the proliferation and metastasis of NSCLC cells by inhibiting the expression of NM23-H1 and is a potential prognostic marker and therapeutic target for NSCLC.