目的:探讨人肺癌细胞转移潜能与KAI1基因表达的关系。方法:应用real-time RTPCR技术检测不同转移潜能的人肺癌细胞株和正常人成纤维细胞株MRC-5中KAI1基因mRNA的表达水平及差异。结果:人肺癌细胞株中KAI1基因mRNA表达水平均显著低于正常肺成纤维细胞株MRC-5；不同转移潜能人大细胞肺癌细胞株中KAI1基因mRNA表达水平有明显差异。结论:KAI1基因表达降低与人肺癌细胞的转移潜能高低有关！
目的 了解“5.12”汶川地震后四川省安县18个乡镇卫生院的卫生人力资源现状，分析存在的问题并提出建议。 方法 2010年11月采用问卷调查方式对安县18个乡镇卫生所进行调查，收集至2009年12月底卫生人力资源情况进行收集，并就调查结果进行分析。 结果 截至2009年12月底，该县医务人员总数为1 249人，其中乡镇卫生所550人，县级医院699人。在乡镇卫生所中，医生272人，护士116人，医护比为2.34︰1；学历以大专、高中及中专为主; 职称以初级及士级为主。 结论 安县卫生服务技术人员学历、职称、专业结构不尽合理，高层次人才短缺的问题，建议完善相关制度及加强全科医生培养工作。
West China Hospital has focused on discipline construction, substantially upgrading research results by strengthening scientific research, and has made great progress in the discipline construction in recent years. This paper aims to explore common various measures for the promotion of construction of state key disciplines, so as to offer a reference for construction of state key disciplines of large-scale, general medical colleges or hospitals.
Objective To systematically assess the efficacy and safety of probiotics in prevention and treatment of bronchial asthma. Methods Randomized controlled trials (RCTs) of probiotics in prevention/treatment of asthma compared with placebo were searched in PubMed, EMbase, Web of Science, OVID and The Cochrane Library published before August 2011. The quality of the included RCTs was evaluated and the data were extracted by two assessors independently. Meta-analyses were performed with RevMan 5.1 software. Results Eleven RCTs on probiotics preventing asthma (n=3 656) and 5 RCTs on probiotics treating asthma (n=430) were identified. The Meta-analyses on preventing asthma showed that probiotics didn’t statistically decease the incidence of asthma (RR=0.76, 95%CI 0.47 to 1.22, P=0.25) and asthma-like wheezing (RR=0.92, 95%CI 0.62 to 1.39, P=0.71) compared with placebo. The Meta-analyses on treating asthma indicated that probiotics could prolong free episodes of asthma (RR=1.48, 95%CI 1.20 to 1.76, Plt;0.000 1) in comparison with placebo. No severe adverse events were found in all included studies. Conclusion The present evidence is not b enough to prove that probiotics is effective to prevent asthma, but it may prolong free episodes of asthma. Although it seems to have the effect on improving lung function, it fails to reduce the acute onset of asthma and has no have the advantage of improving immune function.