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find Keyword "生物材料" 65 results
  • Effect of Aureolysin on Staphylococcus Aureus Biofilm Formation of Dacron Biomaterial Surfaces

    Objective To investigate the effect of aureolysin (Aur) on staphylococcus aureus biofilm formation of dacron biomaterial surfaces under different Aur concentration. Methods Ninety dacron biomaterials were divided into 3 groups (group A, group IA, control group) with random number table (30 piece in each group). Dacron biomaterials were put into vials contained staphylococcus aureus (105 CFU/ml) respectively; then Aur was added to make the concentration at 400ng/ml in group A, and group B at 80ng/ml. The thickness and number of staphylococcus aureus biofilm on the surfaces of dacron biomaterials of each group were evaluated by confocal laser microscopy and scanning electron microscopy after incubating 6h, 16h, 24h, 30h, and 48h. Results The thickness and number of staphylococcus aureus biofilm on dacron biomaterials surfaces increased significantly with time dependence in control group. The thickness and number of staphylococcus aureus biofilm in group A were less than those in group B and control group at each time points (P〈0. 05). The thickness and number in group B were significantly decreased than those in control group (P 〈 0. 05). Conclusion The study shows that Aur can effectively inhibit the formation of staphylococcus aureus biofilm on dacron biomaterials surfaces with dose dependence.

    Release date:2016-08-30 06:18 Export PDF Favorites Scan
  • 组织工程心脏瓣膜构建现状与发展

    摘要 为了克服目前临床应用的人工心脏瓣膜在抗凝和衰坏方面的缺点,随着组织工程学的发展,构建组织工程心脏瓣膜的研究已取得初步进展。综述近年来组织工程心脏瓣膜在种植材料、种子细胞、动物实验和基础研究方面取得的进展,并探讨组织工程心脏瓣膜的优越性和可行性。

    Release date:2016-08-30 06:33 Export PDF Favorites Scan
  • 应用聚乳酸聚乙醇酸膜构建组织工程心脏瓣膜的实验研究

    目的 探讨应用聚乳酸聚乙醇酸(PLGA)构建组织工程心脏瓣膜的可行性. 方法 扫描电子显微镜观察PLGA结构特点,将PLGA在兔皮下包埋,分别于2周、4周、6周、8周和12周观察材料的生物相容性和降解率,培养犬主动脉瓣间质细胞、主动脉壁间质细胞和皮肤成纤维细胞,对照其生长曲线、平滑肌α肌动蛋白表达和扫描电子显微镜特点.将犬主动脉壁间质细胞和内皮细胞种植于PLGA上,观察其形态并测定细胞合成胶原和前列环素的功能. 结果 PLGA呈网孔状结构,孔径179μm.皮下包埋显示PLGA生物相容性好,体内降解时间为12周.犬主动脉瓣间质细胞和主动脉壁间质细胞平滑肌α肌动蛋白均为部分阳性表达,细胞内有大量粗面内质网,生长曲线相似.细胞种植显示细胞在材料表面生长良好,并具有合成胶原和前列环素的功能(P<0.05 ). 结论 以PLGA为支架体外构建组织工程心脏瓣膜细胞不仅能在PLGA表面生长,还能合成细胞间质和血管活性物质,初步提示应用本组材料和方法构建组织工程心脏瓣膜是可行的.

    Release date:2016-08-30 06:34 Export PDF Favorites Scan
  • APPLICATION OF GENIPIN FOR MODIFICATION OF NATURAL BIOMATERIALS AS A CROSSLINKING AGENT

    Objective To review the application of genipin for the modification of natural biomaterials as a crosslinking agent and progress in research. Methods Domestic and foreign literature on application of genipin for the modification of natural biomaterials as a crosslinking agent was thoroughly reviewed. Results Genipin is an effective natural crosslinking agent with a very low level of cytotoxicity compared with conventional synthetic crosslinking agents. Tissues fixed with genipin can maintain a high level of stability as well as resistance to enzymatic degradation. Conclusion Genipin is a promising substitute for conventional synthetic crosslinking agents, which has offered an alternative for modification of natural biomaterials for tissue engineering.

    Release date:2016-08-31 04:07 Export PDF Favorites Scan
  • PROGRESS OF ALGINATE-BASED BIOMEDICAL MATERIALS /WEI

    Objective To review the current situation of alginate-based biomedical materials, especially focus on the clinical strategies and research progress in the clinical applications and point out several key issues that should be concerned about. Methods Based on extensive investigation of domestic and foreign alginate-based biomedical materials research and related patent, literature, and medicine producted, the paper presented the comprehensive analysis of its research and development, application status, and then put forward several new research directions which should be focused on. Results Alginate-based biomedical materials have been widely used in clinical field with a number of patients, but mainly in the fields of wound dressings and dental impression. Heart failure treatment, embolization, tissue engineering, and stem cells culture are expected to become new directions of research and products development. Conclusion Development of alginate-based new products has good clinical feasibility and necessity, but a lot of applied basic researches should be carried out in the further investigations.

    Release date:2016-08-31 04:08 Export PDF Favorites Scan
  • RECENT PROGRESS OF SMALL INTESTINAL SUBMUCOSA IN APPLICATION RESEARCH OF TISSUE REPAIR AND RECONSTRUCTION

    Objective To review the recent progress of the small intestinal submucosa (SIS) in application research of tissue repair and reconstruction. Methods The domestic and international articles on the SIS were reviewed and summarized. Results As a natural extracellular matrix, SIS has outstanding biological advantages, such as good mechanical property, tissue compatibility, and lower immunogenicity. SIS has been used to repair and reconstruct various types of tissue defects in animal models and clinical application, especially in the treatment of hernia, urinary system disease, and refractory skin trauma. The development of the tissue engineering technology expands the field of SIS repair and reconstruction and promotes the intensive study of SIS. However, the long-term effect of SIS in tissue repair and reconstruction still remains to be further observation, while the cell/SIS material construction by tissue engineering technology also needs more studies. Conclusion SIS has a widely promising application future in the tissue repair and reconstruction.

    Release date:2016-08-31 04:12 Export PDF Favorites Scan
  • INFLUENCE OF THREE CENTRAL VENOUS CATHETER BIOMEDICAL MATERIALS ON PROLIFERATION, APOPTOSIS, AND CELL CYCLE OF XUANWEI LUNG CANCER-05 CELLS

    ObjectiveTo explore the influence of three central venous catheter biomedical materials (polyurethane, silicone, and polyvinyl chloride) on the proliferation, apoptosis, and cell cycle of Xuanwei Lung Cancer-05 (XWLC-05) cells so as to provide the basis for clinical choice of central venous catheter. MethodsXWLC-05 cells were cultured and subcultured, and the cells at passage 3 were cultured with polyurethane, silicone, and polyvinyl chloride (1.0 cm × 1.0 cm in size), and only cells served as a control. At 24, 48, and 72 hours after cultured, MTT assay was used to detect the cellular proliferation and flow cytometry to detect the cell cycle and apoptosis. At 72 hours after cultured, inverted microscope was used to observe the cell growth. ResultsInverted microscope showed the cells grew well in control group, polyurethane group, and silicone group. In polyvinyl chloride group, the cells decreased, necrosed, and dissolved; residual adherent cells had morphologic deformity and decreased transmittance. At 24 and 48 hours, no significant difference in proliferation, apoptosis, and cell cycle was found among 4 groups (P gt; 0.05). At 72 hours, the proliferations of XWLC-05 cells in three material groups were significantly inhibited when compared with control group (P lt; 0.05), and the cells in polyvinyl chloride group had more significant proliferation inhibition than polyurethane group and silicone group (P lt; 0.05), but there was no signifcant difference in proliferation inhibition between polyurethane group and silicone group (P gt; 0.05). Compared with the control group, three material groups had significant impact on the rate of apoptosis and cell cycle: polyvinyl chloride group was the most remarkable, followed by silicone group, polyurethane group was minimum (P lt; 0.05). ConclusionPolyvinyl chloride can significantly impact the proliferation, apoptosis, and cell cycle of XWLC-05 cells; polyurethane has better biocompatibility than polyvinyl chloride and silicone

    Release date:2016-08-31 05:39 Export PDF Favorites Scan
  • CURRENT DEVELOPMENT OF BASIC RESEARCH AND CLINICAL USE OF SMALL INTESTINAL SUBMUCOSA

    Objective To summarize the basic research and the cl inical use of small intestinal submucosa (SIS), which is used as a degradable material for tissue repair. Methods Recent l iterature concerning SIS at home and abroad was extensively reviewed, and current developments of the basic research and the cl inical use of SIS were investigated. Results SIShad many biological advantages in tissue repair, and was used to repair various tissue defects in animal trials. It had successful outcomes in many cl inical trials to repair hernia, anal fistula and Peyronie diseases. And it also had good results at the early stage to treat dilation of the anastomosis, urethroplasty, hypospadias, and other diseases, however, the long-term follow-up was needed. Conclusion SIS is one kind of good material for tissue repair, and has promising future in the cl inical use.

    Release date:2016-08-31 05:47 Export PDF Favorites Scan
  • PAST, PRESENT AND FUTURE OF BONE GRAFTING

    Objective To study the past, present and future of bone grafting. Methods Related l iterature on bone grafting in recent years was extensively reviewed. Results Bone grafting had a history over 300 years, a variety of bone grafting candidates including autografting, allografting, xenografting, synthetic and composite bone grafting had been util ized in cl inical orthopedics at present. But bone autografting and allografting represented the preferred alternatives for bone grafting.It would be important trend in bone grafting to fulfill the optimizing design of biomaterials and constructing composite bone substitutes with cells, factors and scaffolds. Conclusion The future bone grafting might be focused on how to achieve the goal of the rapid osseointegration as well as the physiological bone reconstruction.

    Release date:2016-09-01 09:06 Export PDF Favorites Scan
  • FIBRIN-BASED BIOMATERIALS FOR MEDICAL DEVICE

    【Abstract】 Objective To broaden the cl inical uses of fibrin-based biomaterials and to develop further study incell biology and to comprehensively understand and master related knowledge with regard to the present development status of fibrin. Methods Many relevant domestic and international papers were reviewed to make a summary. Results Recognization was obtained from four aspects, which were structure and function of fibrinogen, cl inical use of fibrin, fibrin scaffold for tissue engineering, and compounding biomaterials of fibrin. It showed that every aspect had great research extension and practical appl ication. Conclusion Besides a surgical hemostat and sealant, fibrin has great potentials in playing roles of tissue engineering scaffold, drug del ivery vehicle, and compounding material.

    Release date:2016-09-01 09:09 Export PDF Favorites Scan
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