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find Keyword "酶联免疫吸附法" 2 results
  • 荧光定量聚合酶链反应检测法诊断新生儿乙型肝炎病毒感染的卫生经济学评价

    目的 探讨采用荧光定量聚合酶链反应(FQ-PCR)检测法诊断新生儿乙型肝炎病毒(HBV)感染的经济学成本。 方法 对2010年3月-2010年7月间202例日龄在28 d以内的新生儿采用酶联免疫吸附法检测HBV血清学标志物,对于HBV血清学标志物中除乙型肝炎表面抗原外其余任何一项或一项以上阳性的新生儿采用FQ-PCR检测其血清HBV-DNA的含量,并分析确诊1例HBV感染病例的费用。 结果 血清HBV-DNA水平与HBV标志物表现模式有关,乙型肝炎e抗原(HBeAg)阳性的新生儿,FQ-PCR阳性率为3/16 (18.8%)。HBeAg阴性之新生儿,FQ-PCR阳性率为1/186(5.4‰)。HBeAg阳性病例为进一步明确乙型肝炎病毒含量而进行FQ-PCR检测所产生的费用为587元/例,HBeAg阴性者为20 460元/例。 结论 FQ-PCR确诊HBeAg阳性的新生儿为HBV感染的费用-效益比远高于HBeAg阴性的新生儿,对于HBeAg阴性的新生儿该法不适合大规模的流行病学诊断性研究。

    Release date:2016-09-07 02:34 Export PDF Favorites Scan
  • Comparison and Evaluation of Diagnostic Performance of Four Different Methods for Detection of Anti-dsDNA Antibodies

    ObjectiveTo evaluate diagnostic performance of crithidia luciliae immunofluorescence test (CLIFT), enzyme linked immunosorbent assay (ELISA), linear immunoassay (LIA) and chemiluminescence immunoassay (CLIA) for detection of anti-dsDNA antibodies for systemic lupus erythematosus (SLE). MethodsA total of 178 sera[SLE (n=86), other systemic rheumatic diseases (n=62), and healthy individual (n=30)], from whom received treatment from July 2012 to June 2013, were tested by 4 different assay kits. ResultsThe diagnostic performances of four methods for detecting anti-dsDNA antibodies for SLE were ELISA, CLIA, CLIFT and LIA, from higher to lower; while ELISA had the highest sensitivity (67.4%), and CLIA had the highest specificity (95.6%). The three test methods (ELISA, LIA, CLIA) had almost perfect concordance with the comparison method (CLIFT, Kappa >0.8). With cut-off values set at 95% of specificity, there was no statistical difference of sensitivity between ELISA and CLIA (58.1%, 60.5%; P>0.05). ConclusionFour assays can be used for the clinical detection of anti-dsDNA antibodies, and the results have an almost perfect concordance. Different assays show various performances depending on the methods and cut-off values used.

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