目的:探讨人凋亡相关新基因PNAS-4(hPNAS-4)基因通过脂质体转染至Lewis肺癌LL2细胞后对放射治疗的增敏作用。〖HTH〗方法〖HTSS〗:用脂质体介导的转染技术,将hPNAS-4基因导入Lewis肺癌LL2细胞中,Western blot鉴定其过表达后,观察X射线照射对其集落形成的影响;流式细胞仪检测hPNAS-4基因或／和放疗(0,1,2,4,6 Gy)对LL2细胞生长抑制及凋亡的影响。〖HTH〗结果〖HTSS〗:通过Western blot证实了hPNAS-4基因在LL2细胞中的过表达。Lip-hPNAS-4加放射治疗处理组细胞的生存能力明显降低,肿瘤细胞的克隆形成明显减少,流式细胞术检测体外培养的肿瘤细胞凋亡明显增加。〖HTH〗结论〖HTSS〗:hPNAS-4基因联合放射治疗能产生协同抗肿瘤效应。
ObjectiveTo observe the effects of hydroxysafflor yellow A (HSYA) on microvessel density (MVD) of mice transplanted Lewis lung cancer and mRNA expression of vascular endothelial growth factor (VEGF) so as to explore the tumor-inhibiting mechanism of HSYA. MethodsSixty tumor-bearing C57/BL mice were randomly divided into five groups, with 12 mice in each group, namely a control group, a cyclophosphamide (CTX) group (25mg/kg), a large dose HSYA group (112mg/L), a medium dose HSYA group (56mg/L), and a small dose HSYA group (28mg/L). These different drugs were administered by intraperitoneal injection. The mice were sacrificed 22 days after the treatment. Tumor tissues were sampled and examined by immunohistochemical method and quantitative real-time PCR to detect the expression of MVD and VEGF mRNA. ResultsThe MVD of the medium and small dose HSYA groups and CTX group were 30.01±3.12, 22.56±2.11 and 16.21±2.40, respectively, which were significantly lower than 41.10±2.93 of the control group and 37.66±3.04 of the large dose HSYA group (χ2=2.82, P=0.010;χ2=3.16, P=0.007;χ2=4.58, P=0.000) and (χ2=1.98, χ2=0.038;χ2=2.45, P=0.016;χ2=3.82, P=0.001). The difference in VEGF amplified fluorescence expression threshold between the HSYA groups and the control group was not significant. However, after amplification, the expression of VEGF mRNA in the small dose HSYA group was only 0.43±0.16, which was obviously lower than 0.82±0.06 in the control group (F=0.77, P=0.038). ConclusionHSYA can significantly reduce MVD in mice transplanted Lewis lung cancer and down-regulate expression of VEGF mRNA to achieve tumor-inhibiting effect.