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find Keyword "Ligand" 2 results
  • Expression of Fas/Fas ligand and its relationship with apoptosis in retinoblastoma

    Objective To investigate the correlation of expression of Fas/Fas ligand (FasL) and apoptosis in retinoblastoma (RB). Methods The expression and distribution of Fas/FasL were detected by using immunohistochemical staining in 32 cases of RB. Light microsc opy (32 cases), electron microscopy (4 cases) and TdT mediated biotin-d UTP nick-end labeling (TUNEL) (12 cases) were used to study apoptosis in RB. Results Apoptotic RB cells mostly located at RB regress area. Chromatin margination and apoptotic bodies were found in RB. TUNEL posi tive labeling cells especially located in tumor regress area. Positive immunola beling for Fas and FasL was found in all RB specimens. There was a highly signi ficant and positive correlation between the expression of Fas/FasL and apoptotic indices (AI) (Plt;0.01 or 0.001). Conclusion The results suggest that apoptotic cell death is prevalent in RB and it may be one type of the most dominant cell death. Fas system may play an important role in oncogenesis and progression of RB, and the up-regulation of Fas system expression might induce RB cell apoptosis. (Chin J Ocul Fundus Dis, 2001,17:21-23)

    Release date:2016-09-02 06:03 Export PDF Favorites Scan
  • Experimental Study on PPARγ Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells

    【Abstract】Objective To study the regulatory ability of peroxisome proliferatoractivated receptor γ(PPARγ) ligands to the inflammatory response in human gallbladder epithelial cells. Methods Culture human gallbladder epithelial cells and identify them . Cells were treated for 24 hours with 0, 10 μmol/L, 20 μmol/L, 30 μmol/L, 50 μmol/L and 100 μmol/L of Ciglitazone during cellular growth peak(5th day), then stimulated them with hIL-1β 5 ng/ml for 2 hours and measured the concentration of IL-6、IL-8 and TNF-α in cellular supernatants by riadioimmunoassay. Results Contrasted with control group, the expression of IL-6 and IL-8 in each test group were inhibited (P<0.001). The IL-6 and IL-8 levels were gradually dropped and corelated with the dosage of Cigtitazone, and manifested dosagedependence (P<0.001). The concentration of TNF-α could not be measured. Conclusion PPARγ ligands can inhibit the expression of IL-6 and IL-8 in human gallbladder epithelial cells and probably produce effect in the regulation of cholecystic inflammation.

    Release date:2016-09-08 11:54 Export PDF Favorites Scan
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