Reproducibility of macular ganglion cell-inner plexiform layer measurements using spectral-domain optical coherence tomography_Chinese Journal of Ocular Fundus Diseases

Authors：

XuXiaoyu , XiaoHui , MiLan , 钟毅敏 , 杨莎莎 , 黄洁蕾 ,  刘杏

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, China;

Corresponding author：

刘杏, Email: liuxing@mail.sysu.edu.cn

Keywords：

Macula lutea/anatomy & histology; Retinal ganglion cells; Tomography, optical coherence; Reproducibility of results

DOI：

10.3760/cma.j.issn.1005-1015.2014.06.002

Video：

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Objective To evaluate the repeatability and reproducibility of macular ganglion cell-inner plexiform layer (GCIPL) thickness measurement using spectral-domain optical coherence tomography (Cirrus HD-OCT). Method One hundred and eight eyes of 54 normal subjects (26 males and 28 females) between 19 and 75 years of age were included. Each eye underwent macular scanning using Cirrus HD-OCT Macular Cube 512×128 protocol by two operators. Three scans of each eye were obtained by each operator. For the right eye of each subject, three extra scans were obtained using Macular Cube 200×200 protocol by one operator. The average, minimum, superotemporal, superior, superonasal, inferonasal, inferior, and inferotemporal GCIPL thickness was analyzed and the repeatability of GCIPL thickness measurement was evaluated with intra-operator, inter-operator, intra-protocol, and inter-protocol intraclass correlation coefficients (ICC). Ten extra scans were obtained from the left eyes of 10 randomly selected subjects for reproducibility assessment with coefficients of variation (CV). Results The intra-operator ICC of macular GCIPL measurement using Macular Cube 512×128 protocol by two operators were 0.959-0.995 and 0.954-0.997, respectively; and the inter-operator ICC were 0.944-0.993. All intra-and inter-operator ICC were > 0.800 with the highest and lowest records of the average and minimum GCIPL thickness, respectively. The intra-protocol ICC of Macular Cube 512×128 protocol and Macular Cube 200×200 protocol were 0.986-0.996 and 0.927-0.997, respectively; and the inter-protocol ICC were 0.966-0.994. All intra-and inter-protocol ICC were > 0.800. CV of GCIPL thickness measurement using Macular Cube 512×128 protocol were (0.70±0.31)%-(1.35±0.86)%. Conclusion Cirrus HD-OCT can measure macular GCIPL thickness in normal eyes with excellent repeatability and reproducibility.

Citation： XuXiaoyu, XiaoHui, MiLan. Reproducibility of macular ganglion cell-inner plexiform layer measurements using spectral-domain optical coherence tomography. Chinese Journal of Ocular Fundus Diseases, 2014, 30(6): 540-544. doi: 10.3760/cma.j.issn.1005-1015.2014.06.002 Copy

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2.	Narayanan D, Cheng H, Bonem KN, et al. Tracking changes over time in retinal nerve fiber layer and ganglion cell-inner plexiform layer thickness in multiple sclerosis[J]. Mult Scler, 2014, 20:1331-1341.
3.	刘杏, 曾阳发, 夏园玲, 等.眼前段光学相干断层扫描仪测量前房深度与晶状体厚度的一致性和可重复性研究[J].中国实用眼科杂志, 2007, 25:600-603.
4.	梁远波, 徐亮, 余秋蓉, 等.海德堡视网膜断层扫描仪测量黄斑厚度的可重复性研究[J].中华眼底病杂志, 2005, 21:103-105.
5.	Shrout PE. Measurement reliability and agreement in psychiatry[J]. Stat Methods Med Res, 1998, 7:301-317.
6.	Hopkins WG. Measures of reliability in sports medicine and science[J]. Sports Med, 2000, 30:1-15.
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9.	Medeiros FA, Lisboa R, Weinreb RN, et al. Retinal ganglion cell count estimates associated with early development of visual field defects in glaucoma[J]. Ophthalmology, 2013, 120:736-744.
10.	Schulze A, Lamparter J, Pfeiffer N, et al. Diagnostic ability of retinal ganglion cell complex, retinal nerve fiber layer, and optic nerve head measurements by fourier-domain optical coherence tomography[J]. Graefe's Arch Clin Exp Ophthalmol, 2011, 249:1039-1045.
11.	Mwanza JC, Durbin MK, Budenz DL, et al. Glaucoma diagnostic accuracy of ganglion cell-inner plexiform layer thickness:comparison with nerve fiber layer and optic nerve head[J]. Ophthalmology, 2012, 119:1151-1158.
12.	Jeoung JW, Choi YJ, Park KH, et al. Macular ganglion cell imaging study:glaucoma diagnostic accuracy of spectral-domain optical coherence tomography[J]. Invest Ophthalmol Vis Sci, 2013, 54:4422-4429.
13.	Takayama K, Hangai M, Durbin M, et al. A novel method to detect local ganglion cell loss in early glaucoma using spectral-domain optical coherence tomography[J]. Invest Ophthalmol Vis Sci, 2012, 53:6904-6913.
14.	Choi YJ, Jeoung JW, Park KH, et al. Glaucoma detection ability of ganglion cell-inner plexiform layer thickness by spectral-domain optical coherence tomography in high myopia[J]. Invest Ophthalmol Vis Sci, 2013, 54:2296-2304.
15.	Mwanza JC, Oakley JD, Budenz DL, et al. Macular ganglion cell-inner plexiform layer:automated detection and thickness reproducibility with spectral domain-optical coherence tomography in glaucoma[J]. Invest Ophthalmol Vis Sci, 2011, 52:8323-8329.
16.	黎晓新, 陈玮志.光相干断层扫描检查图像采集及解读分析[J].中华眼底病杂志, 2012, 28:84-88.
17.	蔡正元, 樊莹, 孙晓东, 等.傅立叶光学相干断层扫描测量黄斑区节细胞复合体和视网膜神经纤维层的可重复性研究[J].上海交通大学学报(医学版), 2012, 32:207-210.

1. Vandijk HW, Verbraak FD, Kok PH, et al. Decreased retinal ganglion cell layer thickness in patients with type 1 diabetes[J]. Invest Ophthalmol Vis Sci, 2010, 51:3660-3665.
2. Narayanan D, Cheng H, Bonem KN, et al. Tracking changes over time in retinal nerve fiber layer and ganglion cell-inner plexiform layer thickness in multiple sclerosis[J]. Mult Scler, 2014, 20:1331-1341.
3. 刘杏, 曾阳发, 夏园玲, 等.眼前段光学相干断层扫描仪测量前房深度与晶状体厚度的一致性和可重复性研究[J].中国实用眼科杂志, 2007, 25:600-603.
4. 梁远波, 徐亮, 余秋蓉, 等.海德堡视网膜断层扫描仪测量黄斑厚度的可重复性研究[J].中华眼底病杂志, 2005, 21:103-105.
5. Shrout PE. Measurement reliability and agreement in psychiatry[J]. Stat Methods Med Res, 1998, 7:301-317.
6. Hopkins WG. Measures of reliability in sports medicine and science[J]. Sports Med, 2000, 30:1-15.
7. Harwerth RS, Quigley HA. Visual field defects and retinal ganglion cell losses in patients with glaucoma[J]. Arch Ophthalmol, 2006, 124:853-859.
8. Medeiros FA, Zangwill LM, Bowd C, et al. The structure and function relationship in glaucoma:implications for detection of progression and measurement of rates of change[J]. Invest Ophthalmol Vis Sci, 2012, 53:6939-6946.
9. Medeiros FA, Lisboa R, Weinreb RN, et al. Retinal ganglion cell count estimates associated with early development of visual field defects in glaucoma[J]. Ophthalmology, 2013, 120:736-744.
10. Schulze A, Lamparter J, Pfeiffer N, et al. Diagnostic ability of retinal ganglion cell complex, retinal nerve fiber layer, and optic nerve head measurements by fourier-domain optical coherence tomography[J]. Graefe's Arch Clin Exp Ophthalmol, 2011, 249:1039-1045.
11. Mwanza JC, Durbin MK, Budenz DL, et al. Glaucoma diagnostic accuracy of ganglion cell-inner plexiform layer thickness:comparison with nerve fiber layer and optic nerve head[J]. Ophthalmology, 2012, 119:1151-1158.
12. Jeoung JW, Choi YJ, Park KH, et al. Macular ganglion cell imaging study:glaucoma diagnostic accuracy of spectral-domain optical coherence tomography[J]. Invest Ophthalmol Vis Sci, 2013, 54:4422-4429.
13. Takayama K, Hangai M, Durbin M, et al. A novel method to detect local ganglion cell loss in early glaucoma using spectral-domain optical coherence tomography[J]. Invest Ophthalmol Vis Sci, 2012, 53:6904-6913.
14. Choi YJ, Jeoung JW, Park KH, et al. Glaucoma detection ability of ganglion cell-inner plexiform layer thickness by spectral-domain optical coherence tomography in high myopia[J]. Invest Ophthalmol Vis Sci, 2013, 54:2296-2304.
15. Mwanza JC, Oakley JD, Budenz DL, et al. Macular ganglion cell-inner plexiform layer:automated detection and thickness reproducibility with spectral domain-optical coherence tomography in glaucoma[J]. Invest Ophthalmol Vis Sci, 2011, 52:8323-8329.
16. 黎晓新, 陈玮志.光相干断层扫描检查图像采集及解读分析[J].中华眼底病杂志, 2012, 28:84-88.
17. 蔡正元, 樊莹, 孙晓东, 等.傅立叶光学相干断层扫描测量黄斑区节细胞复合体和视网膜神经纤维层的可重复性研究[J].上海交通大学学报(医学版), 2012, 32:207-210.

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Reproducibility of macular ganglion cell-inner plexiform layer measurements using spectral-domain optical coherence tomography

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