Research Progress of Cell Sheet Technology and Its Applications in Tissue Engineering and Regenerative Medicine_Journal of Biomedical Engineering

Authors：

 MADongyang ¹ , RENLiling ² , MAOTianqiu ³

1. Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou 730050, China;
2. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
3. Departments of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China;

Corresponding author：

MADongyang, Email: doctormdy@hotmail.com

Keywords：

cell sheet technology; tissue engineering; regenerative medicine

DOI：

10.7507/1001-5515.20140220

Video：

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Cell sheet engineering is an important technology to harvest the cultured cells in the form of confluent monolayers using a continuous culture method and a physical approach. Avoiding the use of enzymes, expended cells can be harvested together with endogenous extracellular matrix, cell-matrix contacts, and cell-cell contacts. With high efficiency of cell loading ability and without using exogenous scaffolds, cell sheet engineering has several advantages over traditional tissue engineering methods. In this article, we give an overview on cell sheet technology about its applications in the filed of tissue regeneration, including the construction of soft tissues (corneal, mucous membrane, myocardium, blood vessel, pancreas islet, liver, bladder and skin) and hard tissues (bone, cartilage and tooth root). This techonoly is promising to provide a novel strategy for the development of tissue engineering and regenerative medicine. And further works should be carried out on the operability of this technology and its feasibility to construct thick tissues.

Citation： MADongyang, RENLiling, MAOTianqiu. Research Progress of Cell Sheet Technology and Its Applications in Tissue Engineering and Regenerative Medicine. Journal of Biomedical Engineering, 2014, 31(5): 1164-1167. doi: 10.7507/1001-5515.20140220 Copy

1.	ELLOUMI-HANNACHI I, YAMATO M, OKANO T. Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine[J]. J Intern Med, 2010, 267(1): 54-70.
2.	OKANO T, YAMADA N, SAKAI H, et al. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide)[J]. J Biomed Mater Res, 1993, 27(10): 1243-1251.
3.	NISHIDA K, YAMATO M, HAYASHIDA Y, et al. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium[J]. N Engl J Med, 2004, 351(12): 1187-1196.
4.	MA D, REN L, LIU Y, et al. Engineering scaffold-free bone tissue using bone marrow stromal cell sheets[J]. J Orthop Res, 2010, 28(5): 697-702.
5.	KELM J M, FUSSENEGGER M. Scaffold-free cell delivery for use in regenerative medicine[J]. Adv Drug Deliv Rev, 2010, 62(7-8): 753-764.
6.	OHKI T, YAMATO M, OTA M, et al. Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets[J]. Gastroenterol, 2012, 143(3): 582-588.
7.	HARAGUCHI Y, SHIMIZU T, YAMATO M, et al. Concise review: cell therapy and tissue engineering for cardiovascular disease[J]. Stem Cells Transl Med, 2012, 1(2): 136-141.
8.	ZAKHAROVA L, MASTROENI D, MUTLU N, et al. Transplantation of cardiac progenitor cell sheet onto infarcted heart promotes cardiogenesis and improves function[J]. Cardiovasc Res, 2010, 87(1): 40-49.
9.	KAWAMURA M, MIYAGAWA S, MIKI K, et al. Feasibility, safety, and therapeutic efficacy of human induced pluripotent stem cell-derived cardiomyocyte sheets in a porcine ischemic cardiomyopathy model[J]. Circulation, 2012, 126(11 Suppl 1): S29-S37.
10.	SAITO T, OHASHI K, UTOH R, et al. Reversal of diabetes by the creation of neo-islet tissues into a subcutaneous site using islet cell sheets[J]. Transplantation, 2011, 92(11): 1231-1236.
11.	TURNER A, SUBRAMANIAN R, THOMAS D F, et al. Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty[J]. Eur Urol, 2011, 59(3): 447-454.
12.	HARAGUCHI Y, SHIMIZU T, SASAGAWA T, et al. Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro[J]. Nat Protoc, 2012, 7(5): 850-858.
13.	HARAGUCHI Y, SHIMIZU T, YAMATO M, et al. Regenerative therapies using cell sheet-based tissue engineering for cardiac disease[J]. Cardiol Res Pract, 2011,2011: 845170.
14.	SEKIYA N, MATSUMIYA G, MIYAGAWA S, et al. Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart[J]. J Thorac Cardiovasc Surg, 2009, 138(4): 985-993.
15.	HIBINO N, DUNCAN D R, NALBANDIAN A, et al. Evaluation of the use of an induced puripotent stem cell sheet for the construction of tissue-engineered vascular grafts[J]. J Thorac Cardiovasc Surg, 2012, 143(3): 696-703.
16.	ZHAO J, LIU L, WEI J, et al. A novel strategy to engineer small-diameter vascular grafts from marrow-derived mesenchymal stem cells[J]. Artif Organs, 2012, 36(1): 93-101.
17.	L'HEUREUX N, PÃQUET S, LABBÉ R, et al. A completely biological tissue-engineered human blood vessel[J]. FASEB J, 1998, 12(1): 47-56.
18.	SASAGAWA T, SHIMIZU T, SEKIYA S, et al. Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology[J]. Biomaterials, 2010, 31(7): 1646-1654.
19.	ZHOU Y, CHEN F, HO S T, et al. Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts[J]. Biomaterials, 2007, 28(5): 814-824.
20.	MA D, YAO H, TIAN W, et al. Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model[J]. Clin Oral Implants Res, 2011, 22(10): 1193-1199.
21.	SOLORIO L D, VIEREGGE E L, DHAMI C D, et al. Engineered cartilage via self-assembled hMSC sheets with incorporated biodegradable gelatin microspheres releasing transforming growth factor-β1[J]. J Control Release, 2012, 158(2): 224-232.
22.	YANG B, CHEN G, LI J, et al. Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold[J]. Biomaterials, 2012, 33(8): 2449-2461.
23.	TSUMANUMA Y, IWATA T, WASHIO K, et al. Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model[J]. Biomaterials, 2011, 32(25): 5819-5825.
24.	MA D, REN L, CHEN F, et al. Reconstruction of rabbit critical-size calvarial defect using BMSC sheet[J]. Ann Plastic Surg, 2010, 65(2): 259-265.

1. ELLOUMI-HANNACHI I, YAMATO M, OKANO T. Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine[J]. J Intern Med, 2010, 267(1): 54-70.
2. OKANO T, YAMADA N, SAKAI H, et al. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide)[J]. J Biomed Mater Res, 1993, 27(10): 1243-1251.
3. NISHIDA K, YAMATO M, HAYASHIDA Y, et al. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium[J]. N Engl J Med, 2004, 351(12): 1187-1196.
4. MA D, REN L, LIU Y, et al. Engineering scaffold-free bone tissue using bone marrow stromal cell sheets[J]. J Orthop Res, 2010, 28(5): 697-702.
5. KELM J M, FUSSENEGGER M. Scaffold-free cell delivery for use in regenerative medicine[J]. Adv Drug Deliv Rev, 2010, 62(7-8): 753-764.
6. OHKI T, YAMATO M, OTA M, et al. Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets[J]. Gastroenterol, 2012, 143(3): 582-588.
7. HARAGUCHI Y, SHIMIZU T, YAMATO M, et al. Concise review: cell therapy and tissue engineering for cardiovascular disease[J]. Stem Cells Transl Med, 2012, 1(2): 136-141.
8. ZAKHAROVA L, MASTROENI D, MUTLU N, et al. Transplantation of cardiac progenitor cell sheet onto infarcted heart promotes cardiogenesis and improves function[J]. Cardiovasc Res, 2010, 87(1): 40-49.
9. KAWAMURA M, MIYAGAWA S, MIKI K, et al. Feasibility, safety, and therapeutic efficacy of human induced pluripotent stem cell-derived cardiomyocyte sheets in a porcine ischemic cardiomyopathy model[J]. Circulation, 2012, 126(11 Suppl 1): S29-S37.
10. SAITO T, OHASHI K, UTOH R, et al. Reversal of diabetes by the creation of neo-islet tissues into a subcutaneous site using islet cell sheets[J]. Transplantation, 2011, 92(11): 1231-1236.
11. TURNER A, SUBRAMANIAN R, THOMAS D F, et al. Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty[J]. Eur Urol, 2011, 59(3): 447-454.
12. HARAGUCHI Y, SHIMIZU T, SASAGAWA T, et al. Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro[J]. Nat Protoc, 2012, 7(5): 850-858.
13. HARAGUCHI Y, SHIMIZU T, YAMATO M, et al. Regenerative therapies using cell sheet-based tissue engineering for cardiac disease[J]. Cardiol Res Pract, 2011,2011: 845170.
14. SEKIYA N, MATSUMIYA G, MIYAGAWA S, et al. Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart[J]. J Thorac Cardiovasc Surg, 2009, 138(4): 985-993.
15. HIBINO N, DUNCAN D R, NALBANDIAN A, et al. Evaluation of the use of an induced puripotent stem cell sheet for the construction of tissue-engineered vascular grafts[J]. J Thorac Cardiovasc Surg, 2012, 143(3): 696-703.
16. ZHAO J, LIU L, WEI J, et al. A novel strategy to engineer small-diameter vascular grafts from marrow-derived mesenchymal stem cells[J]. Artif Organs, 2012, 36(1): 93-101.
17. L'HEUREUX N, PÃQUET S, LABBÉ R, et al. A completely biological tissue-engineered human blood vessel[J]. FASEB J, 1998, 12(1): 47-56.
18. SASAGAWA T, SHIMIZU T, SEKIYA S, et al. Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology[J]. Biomaterials, 2010, 31(7): 1646-1654.
19. ZHOU Y, CHEN F, HO S T, et al. Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts[J]. Biomaterials, 2007, 28(5): 814-824.
20. MA D, YAO H, TIAN W, et al. Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model[J]. Clin Oral Implants Res, 2011, 22(10): 1193-1199.
21. SOLORIO L D, VIEREGGE E L, DHAMI C D, et al. Engineered cartilage via self-assembled hMSC sheets with incorporated biodegradable gelatin microspheres releasing transforming growth factor-β1[J]. J Control Release, 2012, 158(2): 224-232.
22. YANG B, CHEN G, LI J, et al. Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold[J]. Biomaterials, 2012, 33(8): 2449-2461.
23. TSUMANUMA Y, IWATA T, WASHIO K, et al. Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model[J]. Biomaterials, 2011, 32(25): 5819-5825.
24. MA D, REN L, CHEN F, et al. Reconstruction of rabbit critical-size calvarial defect using BMSC sheet[J]. Ann Plastic Surg, 2010, 65(2): 259-265.

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