Experimental study on reconstruction of anterior labrum of shoulder joint by chemical extraction of allogeneic tendon and allogeneic chondrocytes_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

CHEN Guofei ¹ , CUI Lei ² , CHEN Peng ² , LI Wei ² , YOU Tian ² , WANG Chen ² ,  JIANG Changqing ² , LIU Gang ¹

1. Department of Traumatic Osteoarthritis, Shenzhen Hospital, University of Chinese Academy of Sciences, Shenzhen Guangdong, 518000, P.R.China;
2. Department of Sports Medicine, Shenzhen Hospital, Peking University, Shenzhen Guangdong, 518036, P.R.China;

Corresponding author：

JIANG Changqing, Email: 853655760@qq.com

Keywords：

Chemical extraction; allogeneic tendon; chondrocytes; glenoid labrum reconstruction; rabbittissue engineering; rabbit

DOI：

10.7507/1002-1892.201911156

Video：

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Objective To study the effect of chemical extraction of allogeneic tendon and allogeneic chondrocytes for reconstruction of anterior labrum of shoulder joint in rabbits.Methods The body weight of 45 adult New Zealand white rabbits ranged from 2.5 to 3.0 kg. The Achilles tendons of 15 rabbits were taken and the allogeneic tendons were prepared by chemical extraction with antigen inactivation. The extracted tendons were compared with untreated tendons by HE and Masson stainings. Chondrocytes were isolated and cultured by trypsin method and identified by immunohistochemical staining of collagen type Ⅱ. The remaining 30 rabbits were used to prepare the model of anterior labrum defect of shoulder joint. After the allogeneic tendon was transplanted to the damaged labrum, the rabbits was randomly divided into two groups (15 in each group). In group A, the allogeneic chondrocytes were injected into the joint immediately after transplantation, while in group B, no treatment was made. At 4, 6, and 8 weeks after operation, 5 transplanted tendons of each group were taken. After general observation, HE staining was used to observe the number of nuclei, Masson staining was used to observe the expression of collagen fibers in muscle fiber tissues, and AB staining was used to detect the glycosaminoglycan level after transplantation, to evaluate the cell growth in the tissues of the two groups of allogeneic tendon.Results By HE and Masson stainings, the allogeneic tendon antigen prepared by chemical extraction method was inactivated and the fibrous tissue structure was intact; collagen type Ⅱ immunohisto-chemistry staining showed that the cultured cells were chondrocytes. After tendon transplantation, the content of glycosaminoglycan in group A was significantly higher than that in group B (P<0.05). At 6 weeks after operation, HE staining showed that the nuclear in tendon tissue of group A was significantly more than that of group B (t=20.043, P=0.000). Masson staining showed that the number of nuclei in tendon tissue of group A was significantly increased, the muscle fibers and collagen fibers were interlaced, the tissue structure was more compact, and the tendon tissue was mainly blue stained; while the number of nuclei in group B was less, mainly collagen fibers of the original graft.Conclusion The allogeneic tendon inactivated by chemical extraction can be used to reconstruct the defect of anterior labrum of shoulder joint in rabbits, and the combination of allogeneic chondrocytes can promote the healing of tendon transplantation.

Citation： CHEN Guofei, CUI Lei, CHEN Peng, LI Wei, YOU Tian, WANG Chen, JIANG Changqing, LIU Gang. Experimental study on reconstruction of anterior labrum of shoulder joint by chemical extraction of allogeneic tendon and allogeneic chondrocytes. Chinese Journal of Reparative and Reconstructive Surgery, 2020, 34(9): 1184-1189. doi: 10.7507/1002-1892.201911156 Copy

1.	Bottoni CR, Wilckens JH, DeBerardino TM, et al. A prospective, randomized evaluation of arthroscopic stabilization versus nonoperative treatment in patients with acute, traumatic, first-time shoulder dislocations. Am J Sports Med, 2002, 30(4): 576-580.
2.	Trojan JD, Meyer LE, Edgar CM, et al. Epidemiology of shoulder instability injuries in collision collegiate sports from 2009 to 2014. Arthroscopy, 2020, 36(1): 36-43.
3.	Kane P, Bifano SM, Dodson CC, et al. Approach to the treatment of primary anterior shoulder dislocation: A review. Phys Sportsmed, 2015, 43(1): 54-64.
4.	Paulos LE, Evans IK, Pinkowski JL. Anterior and anterior-inferior shoulder instability: Treatment by glenoid labrum reconstruction and a modified capsular shift procedure. J Shoulder Elbow Surg, 1993, 2(6): 305-313.
5.	Bateman E, Donald SM. Reconstruction of massive uncontained glenoid defects using a combined autograft-allograft construct with reverse shoulder arthroplasty: preliminary results. J Shoulder Elbow Surg, 2012, 21(7): 925-934.
6.	肖健, 敖英芳, 王健全, 等. 同种异体半月板移植重建肩关节前盂唇的组织学研究. 中国微创外科杂志, 2013, 13(4): 349-351, 356.
7.	潘丕春, 刘金钊, 孙康. γ 射线照射与非照射人肌腱生物力学的比较研究. 中国矫形外科杂志, 2009, 17(13): 1010-1012.
8.	陈鹏, 江长青, 陈国飞, 等. 不同化学萃取次数对同种异体肌腱生物学性能的影响. 实用手外科杂志, 2017, 31(3): 355-358.
9.	Abe H, Itoi E, Yamamoto N, et al. Healing processes of the glenoid labrallesion in a rabbit model of shoulder dislocation. Tohoku J Exp Med, 2012, 228: 103-108.
10.	Provencher MT, Ghodadra N, Romeo AA. Arthroscopic management of anterior instability: pearls, pitfalls, and lessons learned. Orthop Clin North Am, 2010, 41(3): 325-337.
11.	Qu J, van Alphen NA, Thoreson AR, et al. Effects of trypsinization and mineralization on intrasynovial tendon allograft healing to bone. J Orthop Res, 2015, 33(4): 468-474.
12.	谢磊, 陈百成, 王晓峰, 等. 自体及同种异体肌腱重建前交叉韧带术后本体感觉恢复的比较研究. 中国修复重建外科杂志, 2011, 25(8): 907-911.
13.	Song YJ, Hua YH. Tendon allograft for treatment of chronic Achilles tendon rupture: A systematic review. Foot Ankle Surg, 2019, 25(3): 252-257.
14.	吴延平, 王英振, 焦兆德, 等. 反复冻融处理同种异体移植兔跟腱组织学及生物力学变化. 中国组织工程研究与临床康复, 2009, 13(5): 816-820.
15.	姜炜鹏, 左金华, 李继奎, 等. 以 Triton X-100 和脱氧胆酸钠为萃取剂制备兔脱细胞神经基质: 有最佳时间吗? 中国组织工程研究与临床康复, 2009, 13(47): 9241-9244.
16.	王斌, 席智杰, 梁倩倩, 等. 基质诱导自体软骨细胞移植治疗股骨滑车软骨损伤. 中国修复重建外科杂志, 2017, 31(1): 98-104.
17.	林祥龙, 高丽兰, 李瑞欣, 等. 压缩载荷下软骨细胞/复合支架的三维培养. 中国组织工程研究, 2019, 23(10): 1483-1488.
18.	林建华, 陈晓东, 邓凌霄, 等. 大鼠软骨细胞复制性老化的体外观察. 中国修复重建外科杂志, 2007, 21(11): 1228-1232.
19.	Lee SU, Lee JY, Joo SY, et al. Transplantation of a scaffold-free cartilage tissue analogue for the treatment of physeal cartilage injury of the proximal tibia in rabbits. Yonsei Med J, 2016, 57(2): 441-448.
20.	林奕鹏, 李韬, 熊燕, 等. 膝关节自体软骨细胞移植术后康复的研究进展. 中国修复重建外科杂志, 2018, 32(6): 758-763.

1. Bottoni CR, Wilckens JH, DeBerardino TM, et al. A prospective, randomized evaluation of arthroscopic stabilization versus nonoperative treatment in patients with acute, traumatic, first-time shoulder dislocations. Am J Sports Med, 2002, 30(4): 576-580.
2. Trojan JD, Meyer LE, Edgar CM, et al. Epidemiology of shoulder instability injuries in collision collegiate sports from 2009 to 2014. Arthroscopy, 2020, 36(1): 36-43.
3. Kane P, Bifano SM, Dodson CC, et al. Approach to the treatment of primary anterior shoulder dislocation: A review. Phys Sportsmed, 2015, 43(1): 54-64.
4. Paulos LE, Evans IK, Pinkowski JL. Anterior and anterior-inferior shoulder instability: Treatment by glenoid labrum reconstruction and a modified capsular shift procedure. J Shoulder Elbow Surg, 1993, 2(6): 305-313.
5. Bateman E, Donald SM. Reconstruction of massive uncontained glenoid defects using a combined autograft-allograft construct with reverse shoulder arthroplasty: preliminary results. J Shoulder Elbow Surg, 2012, 21(7): 925-934.
6. 肖健, 敖英芳, 王健全, 等. 同种异体半月板移植重建肩关节前盂唇的组织学研究. 中国微创外科杂志, 2013, 13(4): 349-351, 356.
7. 潘丕春, 刘金钊, 孙康. γ 射线照射与非照射人肌腱生物力学的比较研究. 中国矫形外科杂志, 2009, 17(13): 1010-1012.
8. 陈鹏, 江长青, 陈国飞, 等. 不同化学萃取次数对同种异体肌腱生物学性能的影响. 实用手外科杂志, 2017, 31(3): 355-358.
9. Abe H, Itoi E, Yamamoto N, et al. Healing processes of the glenoid labrallesion in a rabbit model of shoulder dislocation. Tohoku J Exp Med, 2012, 228: 103-108.
10. Provencher MT, Ghodadra N, Romeo AA. Arthroscopic management of anterior instability: pearls, pitfalls, and lessons learned. Orthop Clin North Am, 2010, 41(3): 325-337.
11. Qu J, van Alphen NA, Thoreson AR, et al. Effects of trypsinization and mineralization on intrasynovial tendon allograft healing to bone. J Orthop Res, 2015, 33(4): 468-474.
12. 谢磊, 陈百成, 王晓峰, 等. 自体及同种异体肌腱重建前交叉韧带术后本体感觉恢复的比较研究. 中国修复重建外科杂志, 2011, 25(8): 907-911.
13. Song YJ, Hua YH. Tendon allograft for treatment of chronic Achilles tendon rupture: A systematic review. Foot Ankle Surg, 2019, 25(3): 252-257.
14. 吴延平, 王英振, 焦兆德, 等. 反复冻融处理同种异体移植兔跟腱组织学及生物力学变化. 中国组织工程研究与临床康复, 2009, 13(5): 816-820.
15. 姜炜鹏, 左金华, 李继奎, 等. 以 Triton X-100 和脱氧胆酸钠为萃取剂制备兔脱细胞神经基质: 有最佳时间吗? 中国组织工程研究与临床康复, 2009, 13(47): 9241-9244.
16. 王斌, 席智杰, 梁倩倩, 等. 基质诱导自体软骨细胞移植治疗股骨滑车软骨损伤. 中国修复重建外科杂志, 2017, 31(1): 98-104.
17. 林祥龙, 高丽兰, 李瑞欣, 等. 压缩载荷下软骨细胞/复合支架的三维培养. 中国组织工程研究, 2019, 23(10): 1483-1488.
18. 林建华, 陈晓东, 邓凌霄, 等. 大鼠软骨细胞复制性老化的体外观察. 中国修复重建外科杂志, 2007, 21(11): 1228-1232.
19. Lee SU, Lee JY, Joo SY, et al. Transplantation of a scaffold-free cartilage tissue analogue for the treatment of physeal cartilage injury of the proximal tibia in rabbits. Yonsei Med J, 2016, 57(2): 441-448.
20. 林奕鹏, 李韬, 熊燕, 等. 膝关节自体软骨细胞移植术后康复的研究进展. 中国修复重建外科杂志, 2018, 32(6): 758-763.

Chinese Journal of Reparative and Reconstructive Surgery

Experimental study on reconstruction of anterior labrum of shoulder joint by chemical extraction of allogeneic tendon and allogeneic chondrocytes

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