Expression and Clinical Significance of miR-155 in Human Esophageal Squamous Cell Carcinoma_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

WANGXiao-long ^1,2 , CHENXin-tao ¹ , QUGe ¹ , MAHai-bo ¹ , MAJun ³ , LIYin ¹ ,  QINJian-jun ¹

1. Department of Thoracic Surgery, Henan Cancer Hospital, Zhengzhou University, Zhengzhou 540008, P. R. China;
2. Department of Thoracic Surgery, Huaihe Hospital, Henan University, Kaifeng 475000, Henan, P. R. China;
3. Department of Gastroenterology, Institute of Digestive Disease and The Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014, P. R. China;

Corresponding author：

QINJian-jun, Email: qinjianjun73@aliyun.com

Keywords：

miR-155; Esophageal squamous cell carcinoma; Clinicopathological feature

DOI：

10.7507/1007-4848.20150018

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Objective To investigate the expression of MicroRNA 155 (miR-155) in esophageal squamous cell carcinoma (ESCC) and analyze its correlation with clinicopathological features of ESCC. Methods This study included 54 patients with primary ESCC who underwent radical esophagectomy in Department of Thoracic Surgery, Henan Cancer Hospital of Zhengzhou University between January 2010 and November 2012. There were 47 males and 7 females with median age of 61 years (range, 45 to 82 years). Forty patients were in stage Ⅰ or Ⅱ and 14 patients in stage Ⅲ a+b. Expression of miR-155 was determined by SYBR Green qRT-PCR in ESCC tissue and corresponding adjacent normal mucosa in surgical samples from the 54 patients, and its correlation with clinicopathological features was analyzed. Results Expression of miR-155 was significantly lower in ESCC tissue than that in adjacent normal mucosa (Z=-4.258, P=0.000).Expression level of miR-155 was significantly correlated with lymph node metastasis (P=0.040), but not significantly correlated with smoking (P=0.430), drinking (P=0.429), age (P=0.769), gender (P=0.671), depth of invasion (P=0.230), differentiation degree (P=0.896) or pTNM (P=0.407) of ESCC. Conclusion Under-regulation of miR- 155 expression in ESCC tissue may lead to disorders of inflammation response, immune response and relevant tumor suppressor, and may play a significant role in carcinogenesis and progression of ESCC.

Citation： WANGXiao-long, CHENXin-tao, QUGe, MAHai-bo, MAJun, LIYin, QINJian-jun. Expression and Clinical Significance of miR-155 in Human Esophageal Squamous Cell Carcinoma. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2015, 22(1): 61-65. doi: 10.7507/1007-4848.20150018 Copy

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16.	Roldo C, Missiaglia E, Hagan JP, et al. MicroRNA expression abnormalities in pancreatic endocrine and acinar tumor sareassociated with distinctive pathologic features and clinic albehavior. J Clin Oncol, 2006, 24(29):4677-4684.
17.	Dorsett Y, McBride KM, Jankovic M, et al. MicroRNA-155 suppresses activation-induced cytidine deaminase-mediated Myc-Igh translocation. Immunity, 2008, 28(5):630-638.
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24.	Thai TH, Calado DP, Casola S, et al. Regulation of the germinal center response by microRNA-155. Science, 2007, 316(5824):604-608.

1. Kamangar F, Dores GM, Anderson WF. Patterns of cancer incidence, mortality, and prevalence across five continents:defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol, 2006, 24(14):2137-2150.
2. Enzinger PC, Mayer RJ. Esophageal cancer. N Engl J Med, 2003, 349(23):2241-2252.
3. Calin GA, Sevignani C, Dumitru CD, et al. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA, 2004, 101(9):2999-3004.
4. Kluiver J, van den Berg A, de Jong D, et al. Regulation of primicroRNA BIC transcription and processing in Burkitt lymphoma. Oncogene, 2007, 26(26):3769-3776.
5. Ghani S, Riemke P, Schonheit J, et al. Macrophage development from HSCs requires PU. 1-coordinated microRNA expression. Blood, 2011, 118(8):2275-2284.
6. Li Y, Vandenboom TG 2nd, Wang Z, et al. miR-146a suppresses invasion of pancreatic cancer cells. Cancer Res, 2010, 70(4):1486-1495.
7. Jazdzewski K, Liyanarachchi S, Swierniak M, et al. Polymorphic mature microRNAs from passenger strand of pre-miR-146a contribute to thyroid cancer. Proc Natl Acad Sci U S A, 2009, 106(5):1502-1505.
8. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method. Methods, 2001, 25(4):402-408.
9. Llave C, Xie Z, Kasschau KD, et al. Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science, 2002, 297(5589):2053-2056.
10. Lee Y, Ahn C, Han J, et al. The nuclear RNase Ⅲ Drosha initiates microRNA processing. Nature, 2003, 425(6956):415-419.
11. Hobert O. Gene regulation by transcription factors and microRNAs. Science, 2008, 319(5871):1785-1786.
12. Liu Q, Lv GD, Qin X, et al. Role of microRNA let-7 and effect to HMGA2 in esophageal squamous cell carcinoma. Mol Biol Rep, 2012, 39(2):1239-1246.
13. Mori Y, Ishiguro H, Kuwabara Y, et al. MicroRNA-21 induces cell proliferation and invasion in esophageal squamous cell carcinoma. Mol Med Report, 2009, 2(2):235-239.
14. Ma WJ, Lv GD, Tuersun A, et al. Role of microRNA-21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma. Mol Biol Rep, 2011, 38(5):3253-3260.
15. Lu Z, Liu M, Stribinskis V, et al. MicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene. Oncogene, 2008, 27(31):4373-4379.
16. Roldo C, Missiaglia E, Hagan JP, et al. MicroRNA expression abnormalities in pancreatic endocrine and acinar tumor sareassociated with distinctive pathologic features and clinic albehavior. J Clin Oncol, 2006, 24(29):4677-4684.
17. Dorsett Y, McBride KM, Jankovic M, et al. MicroRNA-155 suppresses activation-induced cytidine deaminase-mediated Myc-Igh translocation. Immunity, 2008, 28(5):630-638.
18. Volinia S, Calin GA, Liu CG, et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A, 2006, 103(7):2257-2261.
19. Tili E, Croce CM, Michaille JJ. miR-155:on the crosstalk between inflammation and cancer. Int Rev Immunol, 2009, 28(5):264-284.
20. O'Connell RM, Rao DS, Chaudhuri AA, et al. Physiological and pathological roles for microRNAs in the immune system. Nat Rev Immunol, 2010, 10(2):111-122.
21. O'Connell RM, Taganov KD, Boldin MP, et al. MicroRNA-155 is induced during the macrophage inflammatory response. Proc Natl Acad Sci U S A, 2007, 104(5):1604-1609.
22. Vigorito E, Perks KL, Abreu-Goodger C, et al. microRNA-155 regulates the generation of immunoglobulin class-switched plasma cells. Immunity, 2007, 27(6):847-859.
23. Rodriguez A, Vigorito E, Clare S, et al. Requirement of bic/microRNA-155 for normal immune function. Science, 2007, 316(5824):608-611.
24. Thai TH, Calado DP, Casola S, et al. Regulation of the germinal center response by microRNA-155. Science, 2007, 316(5824):604-608.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Expression and Clinical Significance of miR-155 in Human Esophageal Squamous Cell Carcinoma

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