Effect of lncRNA-MALAT1 on Colorectal Cancer Cells Mediated Angiogenesis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 ZHUDong-liang , YINXiao-ping ,  WANGFang-yuan

Department of Hepatobiliary pancreatic Gastrointestinal Surgery, Xianning Central Hospital, Xianning Tongji Hospital, Xianning 437100, Hubei Province, China;

Corresponding author：

WANGFang-yuan, Email: xnzxyywfy@126.com

Keywords：

lncRNA; Metastasis-associated lung adenocarcinoma transcript 1; Colorectal cancer; Angiogenesis; Vascular endothelial growth factor

DOI：

10.7507/1007-9424.20150015

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To observe the effect of lncRNA-metastasis-associated lung adenocarcinoma transcript 1(MALAT1)on colorectal cancer cells-induced angiogenesis, and explore the potential underlying mechanism. Methods MALAT1 was overexpressed in colorectal cancer cells SW48 by plasmids transfection, then SW48 cells were cultured at normoxia or hypoxia conditions. The culture media was collected, and the concentration of vascular endothelial growth factor (VEGF) in the media was measured by the enzyme-linked immuno sorbent assay (ELISA), and the human umbilical vein endothelial cells (HUVEC) were incubated with the media collected above. Meanwhile, the expression of hypoxia-inducible factor-1α(HIF-1α) in SW48 cells was detected by western blot. Results Overexpression of MALAT1 increased the VEGF level in the culture media, normoxia:the MALAT1 group (514±32) mg/L vs. the control group (110±14) mg/L, P < 0.05; hypoxia:the MALAT1 group (928±18) mg/L vs. the control group (230±21) mg/L, P < 0.05. Meanwhile, the tube formation activity of HUVEC was enhanced, and the expression of HIF-1αwas elevated in the MALAT1 group by western blot. Conclusion Overexpression of MALAT1 could promote colorectal cancer cells-mediated angiogenesis, it may be developed as a new drug target for colorectal cancer treatment.

Citation： ZHUDong-liang, YINXiao-ping, WANGFang-yuan. Effect of lncRNA-MALAT1 on Colorectal Cancer Cells Mediated Angiogenesis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(1): 60-63. doi: 10.7507/1007-9424.20150015 Copy

1.	Yang L, Lin C, Jin C, et al. lncRNA dependent mechanisms of androgen receptor regulated gene activation programs. Nature, 2013, 500(7464):598-602.
2.	刘长, 安公明, 张胜龙, 等.结肠癌组织与癌旁组织长链非编码RNA表达谱的筛选.实用医学杂志, 2014, 30(5):781-782.
3.	Shi X, Sun M, Liu H, et al. Long non-coding RNAs:a new frontier in the study of human diseases. Cancer Lett, 2013, 339(2):159-166..
4.	Jiang Y, Li Y, Fang S, et al. The role of MALAT1 correlates with HPV in cervical cancer. Oncol Lett, 2014, 7(6):2135-2141.
5.	Liu JH, Chen G, Dang YW, et al. Expression and prognostic significance of lncRNA MALAT1 in pancreatic cancer tissues. Asian Pac J Cancer Prev, 2014, 15(7):2971-2977.
6.	Zheng HT, Shi DB, Wang YW, et al. High expression of lncRNA MALAT1 suggests a biomarker of poor prognosis in colorectal cancer. Int J Clin Exp Pathol, 2014, 7(6):3174-3181.
7.	Yang MH, Hu ZY, Xu C, et al. MALAT1 promotes colorectal cancer cell proliferation/migration/invasion via PRKA kinase anchor protein 9. Biochim Biophys Acta, 2014, 1852(1):166-174.
8.	Cho SF, Chang YC, Chang CS, et al. MALAT1 long non-coding RNA is overexpressed in multiple myeloma and may serve as a marker to predict disease progression. BMC Cancer, 2014, 14:809.
9.	Okugawa Y, Toiyama Y, Hur K, et al. Metastasis-associated long non-coding RNA drives gastric cancer development and promotes peritoneal metastasis. Carcinogenesis, 2014, 35(12):2731-2739.
10.	Li B, Chen P, Qu J, et al. Activation of LTBP3 gene by a long noncoding RNA (lncRNA) MALAT1 transcript in mesenchymal stem cells from multiple myeloma. J Biol Chem, 2014, 289(42):29365-29375.
11.	Hayashi H, Arao T, Matsumoto K, et al. Biomarkers of reactive resistance and early disease progression during chemotherapy plus bevacizumab treatment for colorectal carcinoma. Oncotarget, 2014, 5(9):2588-2595.
12.	Kurenova E, Ucar D, Liao J, et al. A FAK scaffold inhibitor disrupts FAK and VEGFR-3 signaling and blocks melanoma growth by targeting both tumor and endothelial cells. Cell Cycle, 2014, 13(16):2542-2553.
13.	吴生华, 俞继卫, 郑林海, 等. CNTN-1、VEGF-C和VEGFR-3在胃癌组织中的表达及其临床意义.中国普外基础与临床杂志, 2011, 18(2):142-148.
14.	李善平, 刘海威, 程乐, 等. VEGF siRNA抑制人胰腺癌PANC-1细胞体外生长的研究.中国普外基础与临床杂志, 2011, 18(6):651-654.
15.	肖衡, 杜成友, 罗诗樵, 等.肿瘤细胞上清液对成纤维细胞表型转变及血管内皮生长因子-A表达的影响.中国普外基础与临床杂志, 2012, 19(2):129-134.
16.	Gutschner T, Hämmerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res, 2013, 73(3):1180-1189.
17.	Zhao Z, Chen C, Liu Y, et al. 17β-Estradiol treatment inhibits breast cell proliferation, migration and invasion by decreasing MALAT-1 RNA level. Biochem Biophys Res Commun, 2014, 445(2):388-393.
18.	Han Y, Liu Y, Zhang H, et al. Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long non-coding RNA MALAT1. FEBS Lett, 2013, 587(23):3875-3882.
19.	Yang F, Yi F, Han X, et al. MALAT-1 interacts with hnRNP C in cell cycle regulation. FEBS Lett, 2013, 587(19):3175-3181.
20.	Han Y, Liu Y, Nie L, et al. Inducing cell proliferation inhibition, apoptosis, and motility reduction by silencing long noncoding ribonucleic acid metastasis-associated lung adenocarcinoma transcript 1 in urothelial carcinoma of the bladder. Urology, 2013, 81(1):209.e1-e7.
21.	Eißmann M, Gutschner T, Hämmerle M, et al. Loss of the abundant nuclear non-coding RNA MALAT1 is compatible with life and development. RNA Biol, 2012, 9(8):1076-1087.
22.	Zhao Y, Yang Y, Trovik J, et al. A novel wnt regulatory axis in endometrioid endometrial cancer. Cancer Res, 2014, 74(18):5103-5117.
23.	Lopez-Ayllon BD, Moncho-Amor V, Abarrategi A, et al. Cancer stem cells and cisplatin-resistant cells isolated from non-small-lung cancer cell lines constitute related cell populations. Cancer Med, 2014, 3(5):1099-1111.
24.	Tee AE, Ling D, Nelson C, et al. The histone demethylase JMJD1A induces cell migration and invasion by up-regulating the expression of the long noncoding RNA MALAT1. Oncotarget, 2014, 5(7):1793-1804.
25.	Wang J, Wang H, Zhang Y, et al. Mutual inhibition between YAP and SRSF1 maintains long non-coding RNA, Malat1-induced tumourigenesis in liver cancer. Cell Signal, 2014, 26(5):1048-1059.

1. Yang L, Lin C, Jin C, et al. lncRNA dependent mechanisms of androgen receptor regulated gene activation programs. Nature, 2013, 500(7464):598-602.
2. 刘长, 安公明, 张胜龙, 等.结肠癌组织与癌旁组织长链非编码RNA表达谱的筛选.实用医学杂志, 2014, 30(5):781-782.
3. Shi X, Sun M, Liu H, et al. Long non-coding RNAs:a new frontier in the study of human diseases. Cancer Lett, 2013, 339(2):159-166..
4. Jiang Y, Li Y, Fang S, et al. The role of MALAT1 correlates with HPV in cervical cancer. Oncol Lett, 2014, 7(6):2135-2141.
5. Liu JH, Chen G, Dang YW, et al. Expression and prognostic significance of lncRNA MALAT1 in pancreatic cancer tissues. Asian Pac J Cancer Prev, 2014, 15(7):2971-2977.
6. Zheng HT, Shi DB, Wang YW, et al. High expression of lncRNA MALAT1 suggests a biomarker of poor prognosis in colorectal cancer. Int J Clin Exp Pathol, 2014, 7(6):3174-3181.
7. Yang MH, Hu ZY, Xu C, et al. MALAT1 promotes colorectal cancer cell proliferation/migration/invasion via PRKA kinase anchor protein 9. Biochim Biophys Acta, 2014, 1852(1):166-174.
8. Cho SF, Chang YC, Chang CS, et al. MALAT1 long non-coding RNA is overexpressed in multiple myeloma and may serve as a marker to predict disease progression. BMC Cancer, 2014, 14:809.
9. Okugawa Y, Toiyama Y, Hur K, et al. Metastasis-associated long non-coding RNA drives gastric cancer development and promotes peritoneal metastasis. Carcinogenesis, 2014, 35(12):2731-2739.
10. Li B, Chen P, Qu J, et al. Activation of LTBP3 gene by a long noncoding RNA (lncRNA) MALAT1 transcript in mesenchymal stem cells from multiple myeloma. J Biol Chem, 2014, 289(42):29365-29375.
11. Hayashi H, Arao T, Matsumoto K, et al. Biomarkers of reactive resistance and early disease progression during chemotherapy plus bevacizumab treatment for colorectal carcinoma. Oncotarget, 2014, 5(9):2588-2595.
12. Kurenova E, Ucar D, Liao J, et al. A FAK scaffold inhibitor disrupts FAK and VEGFR-3 signaling and blocks melanoma growth by targeting both tumor and endothelial cells. Cell Cycle, 2014, 13(16):2542-2553.
13. 吴生华, 俞继卫, 郑林海, 等. CNTN-1、VEGF-C和VEGFR-3在胃癌组织中的表达及其临床意义.中国普外基础与临床杂志, 2011, 18(2):142-148.
14. 李善平, 刘海威, 程乐, 等. VEGF siRNA抑制人胰腺癌PANC-1细胞体外生长的研究.中国普外基础与临床杂志, 2011, 18(6):651-654.
15. 肖衡, 杜成友, 罗诗樵, 等.肿瘤细胞上清液对成纤维细胞表型转变及血管内皮生长因子-A表达的影响.中国普外基础与临床杂志, 2012, 19(2):129-134.
16. Gutschner T, Hämmerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res, 2013, 73(3):1180-1189.
17. Zhao Z, Chen C, Liu Y, et al. 17β-Estradiol treatment inhibits breast cell proliferation, migration and invasion by decreasing MALAT-1 RNA level. Biochem Biophys Res Commun, 2014, 445(2):388-393.
18. Han Y, Liu Y, Zhang H, et al. Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long non-coding RNA MALAT1. FEBS Lett, 2013, 587(23):3875-3882.
19. Yang F, Yi F, Han X, et al. MALAT-1 interacts with hnRNP C in cell cycle regulation. FEBS Lett, 2013, 587(19):3175-3181.
20. Han Y, Liu Y, Nie L, et al. Inducing cell proliferation inhibition, apoptosis, and motility reduction by silencing long noncoding ribonucleic acid metastasis-associated lung adenocarcinoma transcript 1 in urothelial carcinoma of the bladder. Urology, 2013, 81(1):209.e1-e7.
21. Eißmann M, Gutschner T, Hämmerle M, et al. Loss of the abundant nuclear non-coding RNA MALAT1 is compatible with life and development. RNA Biol, 2012, 9(8):1076-1087.
22. Zhao Y, Yang Y, Trovik J, et al. A novel wnt regulatory axis in endometrioid endometrial cancer. Cancer Res, 2014, 74(18):5103-5117.
23. Lopez-Ayllon BD, Moncho-Amor V, Abarrategi A, et al. Cancer stem cells and cisplatin-resistant cells isolated from non-small-lung cancer cell lines constitute related cell populations. Cancer Med, 2014, 3(5):1099-1111.
24. Tee AE, Ling D, Nelson C, et al. The histone demethylase JMJD1A induces cell migration and invasion by up-regulating the expression of the long noncoding RNA MALAT1. Oncotarget, 2014, 5(7):1793-1804.
25. Wang J, Wang H, Zhang Y, et al. Mutual inhibition between YAP and SRSF1 maintains long non-coding RNA, Malat1-induced tumourigenesis in liver cancer. Cell Signal, 2014, 26(5):1048-1059.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of lncRNA-MALAT1 on Colorectal Cancer Cells Mediated Angiogenesis

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content