Role of kinase insert domain containing receptor (KDR) positive cells in formation of cardiospheric structure, myocardium and vessels_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

LI Li , ZHANG Weiran , JIANG Xiaogang , HAN Zhifeng , LIU Chao , LIU Lei ,  WANG Xiaoming

Department of Cardiothoracic Surgery, BenQ Medical Center, the Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, 210019, P.R.China;

Corresponding author：

WANG Xiaoming, Email: wangxm216@163.com

Keywords：

Cardiospheric structure; kinase insert domain containing receptor; cardiogenesis; vasculogenesis; cardiosphere-derived cells

DOI：

10.7507/1007-4848.201701025

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Objective To investigate the role of kinase insert domain containing receptor (KDR) positive cells in the formation of cardiospheric structure, myocardium and vessels.Methods Twenty-four Wistar rats weighting 250 g were selected. Cardiosphere-derived cells were isolated by enzymatic digestion of rat hearts, and their immunological phenotypes were analyzed by using fluorescence-activated cell sorting (FACS). The cardiomyogenic and vasculogenic potential was diagnosed by immunohistochemistry.Results KDR positive cells grew exponentially and formed cell clusters. It also could generate myocardial precursor cells (cardiac troponin T positive). And these cells can develop spontaneous contraction activity in vitro. Meanwhile, KDR positive cells formed many vessel-like structures through a budding process.Conclusion KDR positive cells form cardiospheric structure in vitro culture, and exhibit differentiation potential towards the cardiac and vascular cells. Therefore, KDR positive cells may have a broad prospect of clinical application as cell donors.

Citation： LI Li, ZHANG Weiran, JIANG Xiaogang, HAN Zhifeng, LIU Chao, LIU Lei, WANG Xiaoming. Role of kinase insert domain containing receptor (KDR) positive cells in formation of cardiospheric structure, myocardium and vessels. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2018, 25(4): 333-337. doi: 10.7507/1007-4848.201701025 Copy

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1. Chien KR. Regenerative medicine and human models of human disease. Nature, 2008, 453(7193): 302-305.
2. Leri A, Rota M, Pasqualini FS, et al. Origin of cardiomyocytes in the adult heart. Circ Res, 2015, 116(1): 150-166.
3. Anversa P, Kajstura J, Leri A, et al. Life and death of cardiac stem cells: a paradigm shift in cardiac biology. Circulation, 2006, 113(11): 1451-1463.
4. Laflamme MA, Murry CE. Heart regeneration. Nature, 2011, 473(7347): 326-335.
5. Malliaras K, Makkar RR, Smith RR, et al. Intracoronary cardiosphere-derived cells after myocardial infarction: evidence of therapeutic regeneration in the final 1-year results of the CADUCEUS trial (CArdiosphere-Derived aUtologous stem CElls to reverse ventricUlar dySfunction). J Am Coll Cardiol, 2014, 63(2): 110-122.
6. van Berlo JH, Kanisicak O, Maillet M, et al. c-kit+ cells minimally contribute cardiomyocytes to the heart. Nature, 2014, 509(7500): 337-341.
7. Nelson TJ, Faustino RS, Chiriac A, et al. CXCR4+/FLK-1+ biomarkers select a cardiopoietic lineage from embryonic stem cells. Stem Cells, 2008, 26(6): 1464-1473.
8. 王晓明, 许林海, 孙高忠, 等. 大鼠心包膜组织对其缺血心肌的保护和再生作用. 中华医学杂志, 2011, 91(42): 3012-3015.
9. Leri A, Anversa P. Stem cells: bone-marrow-derived cells and heart failure--the debate goes on. Nat Rev Cardiol, 2013, 10(7): 372-373.
10. de Jong R, Houtgraaf JH, Samiei S, et al. Intracoronary stem cell infusion after acute myocardial infarction: a meta-analysis and update on clinical trials. Circ Cardiovasc Interv, 2014, 7(2): 156-167.
11. Wu SM, Chien KR, Mummery C. Origins and fates of cardiovascular progenitor cells. Cell, 2008, 132(4): 537-543.
12. Chong JJ, Chandrakanthan V, Xaymardan M, et al. Adult cardiac-resident MSC-like stem cells with a proepicardial origin. Cell Stem Cell, 2011, 9(6): 527-540.
13. Bolli R, Chugh AR, D'Amario D, et al. Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomised phase 1 trial. Lancet, 2011, 378(9806): 1847-1857.
14. Yamaguchi TP, Dumont DJ, Conlon RA, et al. flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors. Development, 1993, 118(2): 489-498.
15. Kattman SJ, Huber TL, Keller GM. Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages. Dev Cell, 2006, 11(5): 723-732.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Role of kinase insert domain containing receptor (KDR) positive cells in formation of cardiospheric structure, myocardium and vessels

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