Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

GE Peng ¹ , LI Hanjie ¹ , CHEN Xin ¹ , JING Ruijun ¹ , YAO Yuejuan ¹ , LI Jianzhong ² , ZHANG Weidong ³ ,  YANG Bo ³

1. Department of Cardio-Thoracic Surgery, Second Affiliated Hospital, Xi'an Medical College, Xi'an, 710038, P.R.China;
2. Department of Thoracic Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, P.R.China;
3. Department of Thoracic Surgery, Tianjin First Central Hospital of Nankai University, Tianjin, 300192, P.R.China;

Corresponding author：

YANG Bo, Email: yangbochest@nankai.edu.cn

Keywords：

Lienal polypeptide injection; lipopolysaccharide; acute lung injury; metastasis-associated protein 1

DOI：

10.7507/1007-4848.201803045

Video：

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Objective To analyze the role of lienal polypeptide injection in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods Eighty male SD rats were randomly allocated into 4 groups: a LPS group, a control group, a lienal polypeptide group and a LPS+ lienal polypeptide group (20 rats in each group). Lienal polypeptide or normal saline was given with an intramuscular injection 30 min after an intraperitoneal injection of LPS (5 mg/kg). The severity of pulmonary injury was evaluated 4 h after LPS challenge by enzyme-linked immunosorbent assay (ELISA), wet-to-dry weight ratio, hematoxylin and eosin (HE) staining, TUNEL and Western blotting. Results Lienal polypeptide injection treatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. Moreover lienal polypeptide injection significantly suppressed LPS-induced activation of metastasis-associated protein-1 (MTA1). Conclusion Lienal polypeptide injection is demonstrated to protect rats from LPS-induced acute lung injury by the expression of MTA1.

Citation： GE Peng, LI Hanjie, CHEN Xin, JING Ruijun, YAO Yuejuan, LI Jianzhong, ZHANG Weidong, YANG Bo. Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2019, 26(3): 264-268. doi: 10.7507/1007-4848.201803045 Copy

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2.	Zhou J, Niu G, Pei Y, et al. The effect and clinical efficacy of lienal polypeptide injection combined with FOLFOX chemotherapy regimen in colon cancer patients. Oncol Lett, 2016, 12(5): 3191-3194.
3.	Huang XE, Wang L, Ji ZQ, et al. Safety of lienal polypeptide injection combined with chemotherapy in treating patients with advanced cancer. Asian Pac Cancer Prev, 2015, 16(17): 7837-7841.
4.	苏俊孟, 郭晖, 夏飞, 等. 脾多肽对急性肺损伤大鼠 TLR9 信号相关因子的影响. 贵州医科大学学报, 2017, 42(3): 296-299.
5.	Castillo RL, Carrasco Loza R, Romero-Dapueto C. Pathophysiological approaches of acute respiratory distress syndrome: novel bases for study of lung injury. Open Respir Med J, 2015, 9: 83-91.
6.	Yang B, Ni YF, Wang WC, et al. Melatonin attenuates intestinal ischemia--reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2. Surg Res, 2015, 194(1): 273-280.
7.	杨波, 张卫东, 夏彦民, 等. 盐酸羟考酮注射液对大鼠急性肺损伤的保护作用及机制分析. 中华医学杂志, 2016, 96(31): 2498-2501.
8.	王牧野, 廖辰, 王丽娟, 等. 内皮祖细胞减少肺移植后的缺血-再灌注损伤. 中国胸心血管外科临床杂志, 2018, 25(7): 610-615.
9.	Amodio N, Raimondi L, Juli G, et al. MALAT1: druggable long non-coding RNA for targeted anti-cancer approaches. Hematol Oncol, 2018, 11(1): 63.
10.	Sun X, Xu Y, Zhang S, et al. MicroRNA-183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis-associated protein 1. Exp Ther Med, 2018, 15(6): 5058-5064.
11.	Chen JK, Wang WC, Zang L, et al. Repression of chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouse. Biochem Biophys Res Commun, 2016, 471(4): 515-521.
12.	Li DQ, Pakala SB, Nair SS, et al. Metastasis-associated protein 1/nucleosome remodeling and histone deacetylase complex in cancer. Cancer Res, 2012, 72(2): 387-394.
13.	周福平, 杨喜晶, 王真, 等. 脾多肽注射液联合经导管肝动脉化疗栓塞治疗中晚期原发性肝癌. 第二军医大学学报, 2017, 38(3): 379-382.
14.	严虹霞, 杜娟, 柴丽敏, 等. 不同免疫增强剂对肿瘤患者免疫功能的影响. 中国医院药学杂志, 2017, 37(5): 454-458.
15.	满明昊, 李立宏, 杨彦龙, 等. 颅脑损伤术后细胞免疫指标变化及脾多肽对其影响. 中国临床神经外科杂志, 2016, 21(6): 362-365.
16.	杨波, 张卫东, 李小飞, 等. 大剂量盐酸氨溴索对大鼠肺缺血再灌注损伤后的肺保护机制. 西安交通大学学报 (医学版), 2016, 37(3): 367-371.
17.	陈家宽, 王荣, 李建忠, 等. N-myc 下游调节基因-2 过表达对大鼠肺缺血再灌注损伤的影响. 现代生物医学进展, 2016, 16(15): 2816-2819.

1. Yang B, Li XP, Ni YF, et al. Protective effect of isorhamnetin on lipopolysaccharide-induced acute lung injury in mice. Inflammation, 2016, 39(1): 129-137.
2. Zhou J, Niu G, Pei Y, et al. The effect and clinical efficacy of lienal polypeptide injection combined with FOLFOX chemotherapy regimen in colon cancer patients. Oncol Lett, 2016, 12(5): 3191-3194.
3. Huang XE, Wang L, Ji ZQ, et al. Safety of lienal polypeptide injection combined with chemotherapy in treating patients with advanced cancer. Asian Pac Cancer Prev, 2015, 16(17): 7837-7841.
4. 苏俊孟, 郭晖, 夏飞, 等. 脾多肽对急性肺损伤大鼠 TLR9 信号相关因子的影响. 贵州医科大学学报, 2017, 42(3): 296-299.
5. Castillo RL, Carrasco Loza R, Romero-Dapueto C. Pathophysiological approaches of acute respiratory distress syndrome: novel bases for study of lung injury. Open Respir Med J, 2015, 9: 83-91.
6. Yang B, Ni YF, Wang WC, et al. Melatonin attenuates intestinal ischemia--reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2. Surg Res, 2015, 194(1): 273-280.
7. 杨波, 张卫东, 夏彦民, 等. 盐酸羟考酮注射液对大鼠急性肺损伤的保护作用及机制分析. 中华医学杂志, 2016, 96(31): 2498-2501.
8. 王牧野, 廖辰, 王丽娟, 等. 内皮祖细胞减少肺移植后的缺血-再灌注损伤. 中国胸心血管外科临床杂志, 2018, 25(7): 610-615.
9. Amodio N, Raimondi L, Juli G, et al. MALAT1: druggable long non-coding RNA for targeted anti-cancer approaches. Hematol Oncol, 2018, 11(1): 63.
10. Sun X, Xu Y, Zhang S, et al. MicroRNA-183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis-associated protein 1. Exp Ther Med, 2018, 15(6): 5058-5064.
11. Chen JK, Wang WC, Zang L, et al. Repression of chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouse. Biochem Biophys Res Commun, 2016, 471(4): 515-521.
12. Li DQ, Pakala SB, Nair SS, et al. Metastasis-associated protein 1/nucleosome remodeling and histone deacetylase complex in cancer. Cancer Res, 2012, 72(2): 387-394.
13. 周福平, 杨喜晶, 王真, 等. 脾多肽注射液联合经导管肝动脉化疗栓塞治疗中晚期原发性肝癌. 第二军医大学学报, 2017, 38(3): 379-382.
14. 严虹霞, 杜娟, 柴丽敏, 等. 不同免疫增强剂对肿瘤患者免疫功能的影响. 中国医院药学杂志, 2017, 37(5): 454-458.
15. 满明昊, 李立宏, 杨彦龙, 等. 颅脑损伤术后细胞免疫指标变化及脾多肽对其影响. 中国临床神经外科杂志, 2016, 21(6): 362-365.
16. 杨波, 张卫东, 李小飞, 等. 大剂量盐酸氨溴索对大鼠肺缺血再灌注损伤后的肺保护机制. 西安交通大学学报 (医学版), 2016, 37(3): 367-371.
17. 陈家宽, 王荣, 李建忠, 等. N-myc 下游调节基因-2 过表达对大鼠肺缺血再灌注损伤的影响. 现代生物医学进展, 2016, 16(15): 2816-2819.

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Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Lienal polypeptide injection attenuates lipopolysaccharide-induced acute lung injury in rats by metastasis-associated protein 1

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