Curcumin inhibits Toll-like receptor 4/high mobility group box 1 pathway to protect lipopolysaccharide-induced acute lung injury_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

QIN Zhen ¹ , WANG Bo ¹ , TAN Zhaoxia ¹ ,  LUO Shuhua ²

1. Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China;
2. Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China;

Corresponding author：

LUO Shuhua, Email: drluoshuhua@163.com

Keywords：

Acute lung injury; lipopolysaccharide; high mobility group box 1; Toll-like receptor 4; inflammation factors; curcumin

DOI：

10.7507/1007-4848.202002050

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the protective effect and mechanism of curcumin on lipopolysaccharide (LPS)-induced acute lung injury.Methods Totally 24 SD rats were randomly divided into a control group, a LPS group and a LPS+curcumin group (n=8 in each group). The degree of lung injury (oxygen partial pressure, wet/dry ratio, pathological scores) and inflammatory levels [tumor necrosis factor (TNF)-α, interleukin (IL)-6, monocyte chemotactic protein (MCP)-1, Toll-like receptor 4 (TLR4), mobility group box 1 protein (HMGB1) expression] of the lung were detected in different groups.Results Oxygen partial pressure was significantly lower in the LPS group than that in the control group (P<0.05), while wet/dry ratio, pathological scores and expression levels of TNF-α, IL-6, MCP-1, TLR4 and HMGB1 were significantly higher in the LPS group than those in the control group (P<0.05). Compared with the LPS group, curcumin significantly reduced wet/dry ratio, pathological scores and expression levels of TNF-α, IL-6, MCP-1, TLR4 and HMGB1 in the LPS+curcumin group (P<0.05), while it significantly improved oxygen partial pressure (P<0.05).Conclusion Curcumin might protect LPS-induced acute lung injury through inhibition of TLR4-HMGB1-inflammation pathway.

Citation： QIN Zhen, WANG Bo, TAN Zhaoxia, LUO Shuhua. Curcumin inhibits Toll-like receptor 4/high mobility group box 1 pathway to protect lipopolysaccharide-induced acute lung injury. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2020, 27(6): 685-688. doi: 10.7507/1007-4848.202002050 Copy

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7.	Kim J, Jeong SW, Quan H, et al. Effect of curcumin (curcuma longa extract) on LPS-induced acute lung injury is mediated by the activation of AMPK. J Anesth, 2016, 30(1): 100-108.
8.	Płóciennikowska A, Hromada-Judycka A, Borzęcka K, et al. Co-operation of TLR4 and raft proteins in LPS-induced pro-inflammatory signaling. Cell Mol Life Sci, 2015, 72(3): 557-581.
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11.	Shang L, Wang L, Shi X, et al. HMGB1 was negatively regulated by HSF1 and mediated the TLR4/MyD88/NF-κB signal pathway in asthma. Life Sci, 2020, 241: 117120.
12.	石青青, 苏湘川, 杨小平, 等. 基于JAK2/STAT3信号通路探讨姜黄素改善脂多糖诱导的小鼠急性肺损伤的作用研究. 中国中医急症, 2019, 28(5): 797-800.
13.	陈永锋, 周向东, 杨和平. 姜黄素对内毒素诱导的急性肺损伤的保护作用及机制研究. 第三军医大学学报, 2014, 36(14): 1492-1495.
14.	邵奇, 王倩梅, 马善波, 等. 姜黄素对急性肺损伤小鼠炎症反应的改善作用. 中成药, 2019, 41(10): 2349-2353.

1. Fan E, Brodie D, Slutsky AS. Acute respiratory distress syndrome: advances in diagnosis and treatment. JAMA, 2018, 319(7): 698-710.
2. Patel VJ, Biswas Roy S, Mehta HJ, et al. Alternative and natural therapies for acute lung injury and acute respiratory distress syndrome. Biomed Res Int, 2018, 2018: 2476824.
3. He Y, Yue Y, Zheng X, et al. Curcumin, inflammation, and chronic diseases: how are they linked? Molecules, 2015, 20(5): 9183-9213.
4. Jantan I, Bukhari SN, Lajis NH, et al. Effects of diarylpentanoid analogues of curcumin on chemiluminescence and chemotactic activities of phagocytes. J Pharm Pharmacol, 2012, 64(3): 404-412.
5. 肖正华, 古君, 秦超毅, 等. 乌司他丁对机械通气相关性肺损伤的保护作用及其机制研究. 中国胸心血管外科临床杂志, 2016, 23(10): 1001-1005.
6. Cheng K, Yang A, Hu X, et al. Curcumin attenuates pulmonary inflammation in lipopolysaccharide induced acute lung injury in neonatal rat model by activating peroxisome proliferator-activated receptor γ (PPARγ) pathway. Med Sci Monit, 2018, 24: 1178-1184.
7. Kim J, Jeong SW, Quan H, et al. Effect of curcumin (curcuma longa extract) on LPS-induced acute lung injury is mediated by the activation of AMPK. J Anesth, 2016, 30(1): 100-108.
8. Płóciennikowska A, Hromada-Judycka A, Borzęcka K, et al. Co-operation of TLR4 and raft proteins in LPS-induced pro-inflammatory signaling. Cell Mol Life Sci, 2015, 72(3): 557-581.
9. Andersson U, Yang H, Harris H. Extracellular HMGB1 as a therapeutic target in inflammatory diseases. Expert Opin Ther Targets, 2018, 22(3): 263-277.
10. Tangerås LH, Silva GB, Stødle GS, et al. Placental inflammation by HMGB1 activation of TLR4 at the syncytium. Placenta, 2018, 72-73: 53-61.
11. Shang L, Wang L, Shi X, et al. HMGB1 was negatively regulated by HSF1 and mediated the TLR4/MyD88/NF-κB signal pathway in asthma. Life Sci, 2020, 241: 117120.
12. 石青青, 苏湘川, 杨小平, 等. 基于JAK2/STAT3信号通路探讨姜黄素改善脂多糖诱导的小鼠急性肺损伤的作用研究. 中国中医急症, 2019, 28(5): 797-800.
13. 陈永锋, 周向东, 杨和平. 姜黄素对内毒素诱导的急性肺损伤的保护作用及机制研究. 第三军医大学学报, 2014, 36(14): 1492-1495.
14. 邵奇, 王倩梅, 马善波, 等. 姜黄素对急性肺损伤小鼠炎症反应的改善作用. 中成药, 2019, 41(10): 2349-2353.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Curcumin inhibits Toll-like receptor 4/high mobility group box 1 pathway to protect lipopolysaccharide-induced acute lung injury

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