Influence on the expression of SPARC and GLUT-4 protein in adipose tissue ofnon-obese GK rats after gastric bypass operation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAO Dan ¹ , ZHANG Pengbo ² , GONG Shuai ² , LIU Li ² , WANG Jike ² ,  REN Zeqiang ²

1. Department of Gastrointestinal Surgery, The Second People’s Hospital of Huai’an, Huai’an, Jiangsu 223000, P. R. China;
2. Department of Pancreatic Surgery, The Affiliated Hospital of Xuzhou Meidcal University, Xuzhou, Jiangsu 221000, P. R. China;

Corresponding author：

REN Zeqiang, Email: rzq0805@189.cn

Keywords：

gastric bypass operation; secreted protein acidic and rich in cysteine; glucose transporter-4; insulin resistance

DOI：

10.7507/1007-9424.201908091

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Objective To investigate the expressions of glucose transporter-4 (GLUT-4) and secreted protein acidic and rich in cysteine (SPARC) in adipose tissue of Goto-Kakizaki (GK)/Wister rats after gastric bypass operation (GBP), and to explore the possible mechanism of GBP improving insulin resistance.Methods Healthy male GK rats were randomly divided into diabetic operation group (DO group, underwent GBP), diabetic sham operation group (DS group, underwent sham-operation), and diabetic control group (DC group, received no-treatment), Wister rats were set as normal control group (NC group, received no-treatment). The weight, fasting blood glucose (FPG), fasting serum insulin (Fins), and HbA1c were measured before operation and at the 1st, 2nd, 4th, and 8th week after operation. Quantitative insulin sensitivity check index (QUICKI) was measured in before operation and at the 8th week after operation, and the expressions of GLUT-4 and SPARC protein in adipose tissues were measured by Western Blot method at the 8th week after operative too.Results ① Weight: the weight of the DO group was lower than preoperative at the 2nd and 4th week after GBP (P<0.05), but increased to the normal level at the 8th week after GBP (P>0.05). The weight of the DO group was lower than those of the DS group, DC group, and NC group at the same time point of 2nd, 4th, and 8th week (P<0.05). ② FPG: the FPG level of the DO group was lower than preoperative at the 2nd, 4th, and 8th week after GBP (P<0.05), and lower than those of the DS group and the DC group from 2nd to 8th week after GBP (P<0.05). The FPG level between the DS group and the DC group had no statistical significance (P>0.05). ③ Fins: the Fins level of the DO group was higher than preoperative at the 2nd week after GBP (P<0.05), and decreased gradually at 4th and 8th week but not significantly differed from the NC group at the same time point. At the 2nd week after GBP, the Fins level of the DO group was higher than those of the DS group and the DC group (P<0.05), but there was no statistical significance between the DS group and the DC group (P>0.05). ④ HbA1c: the HbA1c level of the DO group started to decrease but there was no statistical significance between preoperative and all time after GBP (P>0.05). There was no statistical significance among the 4 groups at the 8th week after GBP (P>0.05). ⑤ QUICKI: at the 8th week, the QUICKI value of the DO group was higher than preoperative (P<0.05), and at the same time, the QUICKI value of the DO group and the NC group were higher than those of the DS group and the DC group (P<0.05), but there was no statistical significance between the DO group and the NC group (P>0.05), as well as the DS group and the DC group (P>0.05). ⑥ GLUT-4 protein and SPARC protein: the expression of GLUT-4 protein of the DO group was dramatically higher than those of the DC, DS, and NC group (P<0.05), and the expression of SPARC protein of the DO group was dramatically lower than those of the DC, DS, and NC group (P<0.05) at the 8th week. But the expressions of GLUT-4 and SPARC protein had no statistical significance among the DS, DC, and NC group at the 8th week after GBP (P>0.05).Conclusion GBP may improve and increase the sensitivity of insulin resistance by regulating the expressions of GLUT-4 and SPARC protein in adipose tissue of GK rats.

Citation： YAO Dan, ZHANG Pengbo, GONG Shuai, LIU Li, WANG Jike, REN Zeqiang. Influence on the expression of SPARC and GLUT-4 protein in adipose tissue ofnon-obese GK rats after gastric bypass operation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2020, 27(6): 685-690. doi: 10.7507/1007-9424.201908091 Copy

1.	Pories WJ, Swanson MS, MacDonald KG, et al. Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg, 1995, 222(3): 339-350.
2.	温雨晴, 谭迎春, 张蓬波, 等. 胃转流术对 2 型糖尿病大鼠脂肪组织胰岛素抵抗的影响. 中华实验外科杂志, 2013, 30(11): 2324-2326.
3.	张蓬波, 陈守坤, 张秀忠, 等. 胃转流术对 2 型糖尿病大鼠肝组织胰岛素受体底物-2、葡萄糖转运体-2 表达的影响. 中华实验外科杂志, 2013, 30(12): 2587-2589.
4.	张秀忠, 任泽强, 张蓬波. 胃转流术对 2 型糖尿病大鼠的降糖作用及对糖耐量和胰岛素抵抗的影响. 中华实验外科杂志, 2010, 27(12): 1892-1894.
5.	龚帅, 任泽强, 张蓬波, 等. 胃转流术对非肥胖型 2 型糖尿病大鼠骨骼肌胰岛素受体底物-1 及泛素化蛋白表达的影响. 中国普外基础与临床杂志, 2016, 23(8): 915-921.
6.	Shao J, Yamashita H, Qiao L, et al. Decreased Akt kinase activity and insulin resistance in C57BL/KsJ-Leprdb/db mice. J Endocrinol, 2000, 167(1): 107-115.
7.	谭柏宏, 张立海, 王跃生, 等. 胃转流术对 2 型糖尿病大鼠棕色脂肪组织的影响. 黑龙江医药科学, 2018, 41(1): 39-40, 34.
8.	Yu H, Li Q, Zhang M, et al. Decreased leptin is associated with alterations in thyroid-stimulating hormone levels after Roux-en-Y gastric bypass surgery in obese euthyroid patients with type 2 diabetes. Obes Facts, 2019, 12(3): 272-280.
9.	李晗, 高宏凯, 张新国, 等. 胃转流手术后 GK 大鼠血糖变化与摄食量及体质量的关系. 中华实验外科杂志, 2010, 27(8): 1069-1070.
10.	张强, 陈轩, 徐亮. 胃转流术治疗 2 型糖尿病与 JNK 信号通路的关系. 中国普外基础与临床杂志, 2018, 25(9): 1055-1059.
11.	Xu J, Yang S, Gu X, et al. SPARC correlates with unfavorable outcome and promotes tumor growth in lung squamous cell carcinoma. Exp Mol Pathol, 2019, 110: 104276.
12.	黄海波, 赵燕, 杨树军. 富含半胱氨酸的酸性分泌蛋白在食管癌中的研究进展. 国际肿瘤学杂志, 2014, 41(12): 918-921.
13.	申玉翠, 张其胜, 姚忆蓉, 等. 结肠癌组织 SPARC 蛋白质的表达及其临床意义. 实用医学杂志, 2014, 30(20): 3277-3279.
14.	孙烈, 陈国卫, 尹杰, 等. 富含半胱氨酸的酸性分泌蛋白对胰腺癌细胞增殖、迁移和侵袭能力的影响. 中华实验外科杂志, 2014, 31(9): 1954-1956.
15.	Jiang X, Liu F, Wang Y, et al. Secreted protein acidic and rich in cysteine promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells and acquisition of cancer stem cell phenotypes. J Gastroenterol Hepatol, 2019, 34(10): 1860-1868.
16.	Frangogiannis NG. Matricellular proteins in cardiac adaptation and disease. Physiol Rev, 2012, 92(2): 635-688.
17.	罗丽颖, 吴佳慧, 项潇琼, 等. 富含半胱氨酸的酸性分泌蛋白对青光眼滤过泡瘢痕化影响的研究进展. 上海交通大学学报: 医学版, 2019, 39(6): 684-688.
18.	刘锴, 宋海燕, 刘姝, 等. 2 型糖尿病肥胖患者 SPARC 表达差异性研究. 中华内分泌代谢杂志, 2014, 30(5): 388-392.
19.	Takahashi M, Nagaretani H, Funahashi T, et al. The expression of SPARC in adipose tissue and its increased plasma concentration in patients with coronary artery disease. Obes Res, 2001, 9(7): 388-393.
20.	Aoi W, Hirano N, Lassiter DG, et al. Secreted protein acidic and rich in cysteine (SPARC) improves glucose tolerance. FASEB J, 2019, 33(9): 10551-10562.
21.	Tartare-Deckert S, Chavey C, Monthouel MN, et al. The matri-cellular protein SPARC/osteonectin as a newly identified factor up-regulated in obesity. J Biol Chem, 2001, 276(25): 22231-22237.
22.	Nie J, Sage EH. SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling. J Biol Chem, 2009, 284(2): 1279-1290.
23.	Harries LW, McCulloch LJ, Holley JE, et al. A role for SPARC in the moderation of human insulin secretion. PLoS One, 2013, 8(6): e68253.
24.	Song H, Guan Y, Zhang L, et al. SPARC interacts with AMPK and regulates GLUT4 expression. Biochem Biophys Res Commun, 2010, 396(4): 961-966.
25.	Jing Y, Jin Y, Wang Y, et al. SPARC promotes the proliferation and metastasis of oral squamous cell carcinoma by PI3K/AKT/PDGFB/PDGFRβ axis. J Cell Physiol, 2019, [Epub ahead of print].

1. Pories WJ, Swanson MS, MacDonald KG, et al. Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg, 1995, 222(3): 339-350.
2. 温雨晴, 谭迎春, 张蓬波, 等. 胃转流术对 2 型糖尿病大鼠脂肪组织胰岛素抵抗的影响. 中华实验外科杂志, 2013, 30(11): 2324-2326.
3. 张蓬波, 陈守坤, 张秀忠, 等. 胃转流术对 2 型糖尿病大鼠肝组织胰岛素受体底物-2、葡萄糖转运体-2 表达的影响. 中华实验外科杂志, 2013, 30(12): 2587-2589.
4. 张秀忠, 任泽强, 张蓬波. 胃转流术对 2 型糖尿病大鼠的降糖作用及对糖耐量和胰岛素抵抗的影响. 中华实验外科杂志, 2010, 27(12): 1892-1894.
5. 龚帅, 任泽强, 张蓬波, 等. 胃转流术对非肥胖型 2 型糖尿病大鼠骨骼肌胰岛素受体底物-1 及泛素化蛋白表达的影响. 中国普外基础与临床杂志, 2016, 23(8): 915-921.
6. Shao J, Yamashita H, Qiao L, et al. Decreased Akt kinase activity and insulin resistance in C57BL/KsJ-Leprdb/db mice. J Endocrinol, 2000, 167(1): 107-115.
7. 谭柏宏, 张立海, 王跃生, 等. 胃转流术对 2 型糖尿病大鼠棕色脂肪组织的影响. 黑龙江医药科学, 2018, 41(1): 39-40, 34.
8. Yu H, Li Q, Zhang M, et al. Decreased leptin is associated with alterations in thyroid-stimulating hormone levels after Roux-en-Y gastric bypass surgery in obese euthyroid patients with type 2 diabetes. Obes Facts, 2019, 12(3): 272-280.
9. 李晗, 高宏凯, 张新国, 等. 胃转流手术后 GK 大鼠血糖变化与摄食量及体质量的关系. 中华实验外科杂志, 2010, 27(8): 1069-1070.
10. 张强, 陈轩, 徐亮. 胃转流术治疗 2 型糖尿病与 JNK 信号通路的关系. 中国普外基础与临床杂志, 2018, 25(9): 1055-1059.
11. Xu J, Yang S, Gu X, et al. SPARC correlates with unfavorable outcome and promotes tumor growth in lung squamous cell carcinoma. Exp Mol Pathol, 2019, 110: 104276.
12. 黄海波, 赵燕, 杨树军. 富含半胱氨酸的酸性分泌蛋白在食管癌中的研究进展. 国际肿瘤学杂志, 2014, 41(12): 918-921.
13. 申玉翠, 张其胜, 姚忆蓉, 等. 结肠癌组织 SPARC 蛋白质的表达及其临床意义. 实用医学杂志, 2014, 30(20): 3277-3279.
14. 孙烈, 陈国卫, 尹杰, 等. 富含半胱氨酸的酸性分泌蛋白对胰腺癌细胞增殖、迁移和侵袭能力的影响. 中华实验外科杂志, 2014, 31(9): 1954-1956.
15. Jiang X, Liu F, Wang Y, et al. Secreted protein acidic and rich in cysteine promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells and acquisition of cancer stem cell phenotypes. J Gastroenterol Hepatol, 2019, 34(10): 1860-1868.
16. Frangogiannis NG. Matricellular proteins in cardiac adaptation and disease. Physiol Rev, 2012, 92(2): 635-688.
17. 罗丽颖, 吴佳慧, 项潇琼, 等. 富含半胱氨酸的酸性分泌蛋白对青光眼滤过泡瘢痕化影响的研究进展. 上海交通大学学报: 医学版, 2019, 39(6): 684-688.
18. 刘锴, 宋海燕, 刘姝, 等. 2 型糖尿病肥胖患者 SPARC 表达差异性研究. 中华内分泌代谢杂志, 2014, 30(5): 388-392.
19. Takahashi M, Nagaretani H, Funahashi T, et al. The expression of SPARC in adipose tissue and its increased plasma concentration in patients with coronary artery disease. Obes Res, 2001, 9(7): 388-393.
20. Aoi W, Hirano N, Lassiter DG, et al. Secreted protein acidic and rich in cysteine (SPARC) improves glucose tolerance. FASEB J, 2019, 33(9): 10551-10562.
21. Tartare-Deckert S, Chavey C, Monthouel MN, et al. The matri-cellular protein SPARC/osteonectin as a newly identified factor up-regulated in obesity. J Biol Chem, 2001, 276(25): 22231-22237.
22. Nie J, Sage EH. SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling. J Biol Chem, 2009, 284(2): 1279-1290.
23. Harries LW, McCulloch LJ, Holley JE, et al. A role for SPARC in the moderation of human insulin secretion. PLoS One, 2013, 8(6): e68253.
24. Song H, Guan Y, Zhang L, et al. SPARC interacts with AMPK and regulates GLUT4 expression. Biochem Biophys Res Commun, 2010, 396(4): 961-966.
25. Jing Y, Jin Y, Wang Y, et al. SPARC promotes the proliferation and metastasis of oral squamous cell carcinoma by PI3K/AKT/PDGFB/PDGFRβ axis. J Cell Physiol, 2019, [Epub ahead of print].

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence on the expression of SPARC and GLUT-4 protein in adipose tissue ofnon-obese GK rats after gastric bypass operation

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