Mechanism of Sodium Aescinate in Preventing Postoperative Intestinal Adhesion in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 XULi ¹ , SUNHui-ping ¹ , LIDong-mei ¹ , LIXia ¹ ,  CAOJun-ping ²

1. Department of Pharmacy, General Hospital of Armed Police Forces, Beijing 100039, China;
2. Department of Sterilization and Supply, General Hospital of Armed Police Forces, Beijing 100039, China;

Corresponding author：

CAOJun-ping, Email: dfbb_108@163.com

Keywords：

Sodium aescinate; Intestinal adhesion; Tumor necrosis factor-α; Interleukin-1β; Interleukin

DOI：

10.7507/1007-9424.20140231

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Objective To investigate the anti-inflammatory mechanism of sodium aescinate in preventing postoperative intestinal adhesion in rats. Methods The SD rats were subjected to operation for establishing intestinal adhesion models, then randomly divided into model group, dexamethasone group(dexamethasone, i.v. 5 mg/kg), and sodium aescinate group(sodium aescinate, i.v. 2 mg/kg), 10 rats in each group. Another ten normal rats were selected as sham operation group. One times administration was administered on day 1 before establishing adhesion model, and administration for 3 d after modeling, once a day. On day 7 after operation, all of the rats were killed. The intestinal adhesion was graded and the adhesive tissues were taken for hydroxyproline determination. The levels of tumor necrosis factor(TNF)-α, interleukin(IL)-1β, and IL-6 in the serum were detected by ELISA. Results Compared with the model group, sodium aescinate could obviously improve the severity of postoperative adhesion, markedly decrease hydroxyproline content in the adhesive tissues(P < 0.01), and significantly inhibit the levels of TNF-α, IL-1β, and IL-6 in the serum(P < 0.01). Conclusion Sodium aescinate could effectively prevent the formation of postoperative intestinal adhesion by inhibiting the expressions of inflammatory cytokines and decreasing the inflammatory response.

Citation： XULi, SUNHui-ping, LIDong-mei, LIXia, CAOJun-ping. Mechanism of Sodium Aescinate in Preventing Postoperative Intestinal Adhesion in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(8): 957-959. doi: 10.7507/1007-9424.20140231 Copy

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2.	Hellebrekers BW, Kooistra T. Pathogenesis of postoperative adhesion formation[J]. Br J Surg, 2011, 98(11): 1503-1516.
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4.	Li X, Chen GP, Li L, et al. Dual effects of sodium aescinate on vascular tension in rat thoracic aorta[J]. Microvasc Res, 2010, 79(1): 63-69.
5.	Wang YK, Han J, Xiong WJ, et al. Evaluation of in vivo antioxidant and immunity enhancing activities of sodium aescinate injection liquid[J]. Molecules, 2012, 17(9): 10267-10275.
6.	李冬梅, 徐丽, 曹军平, 等.七叶皂苷钠预防术后肠粘连的实验和临床研究[J].世界中医药, 2013, 8(10): 1164-1165, 1168.
7.	Phillips RK, Dudley HA. The effect of tetracycline lavage and trauma on visceral and parietal peritoneal ultrastructure and adhesion formation[J]. Br J Surg, 1984, 71(7): 537-539.
8.	王春霞.常通口服液对炎症因子参与大鼠肠粘连的影响[J].中国药师, 2005, 8(7): 535-537.
9.	Imudia AN, Kumar S, Saed GM, et al. Pathogenesis of intraabdominal and pelvic adhesion development[J]. Semin Reprod Med, 2008, 26(4): 289-297.
10.	Ozoğul Y, Baykal A, Onat D, et al. An experimental study of the effect of aprotinin on intestinal adhesion formation[J]. Am J Surg, 1998, 175(2): 137-141.
11.	隋新兵, 张勤, 裘华森, 等.丹参多酚酸盐预防大鼠术后肠粘连的机制[J].中西医结合学报, 2007, 5(5): 521-525.
12.	Kosaka H, Yoshimoto T, Yoshimoto T, et al. Interferon-gamma is a therapeutic target molecule for prevention of postoperative adhesion formation[J]. Nat Med, 2008, 14(4): 437-441.
13.	Franke A, Lante W, Kurig E, et al. Postoperative PMBC-derived IL-6 and TNF-alpha-release is uninfluenced by IL-12-mediated restoration of IFN-gamma synthesis[J]. Scand Cardiovasc J, 2009, 43(2): 136-144.
14.	Kawashima R, Kawamura YI, Oshio T, et al. Comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy[J]. J Immunoassay Immunochem, 2012, 33(3): 291-301.
15.	Kaidi AA, Gurchumelidze T, Nazzal M, et al. Tumor necrosis factor-α: a marker for peritoneal adhesion formation[J]. J Surg Res, 1995, 58(5): 516-518.
16.	Kurukahvecioglu O, Koksal H, Gulbahar O, et al. Infliximab "TNF-alpha antagonist" decreases intraabdominal adhesions[J]. Saudi Med J, 2007, 28(12): 1830-1835.
17.	Bessler H, Shavit Y, Mayburd E, et al. Postoperative pain, morphine consumption, and genetic polymorphism of IL-1beta and IL-1 receptor antagonist[J]. Neurosci Lett, 2006, 8(4): 190.
18.	Saba AA, Godziachvili V, Mavani AK, et al. Serum levels of interleukin 1 and tumor necrosis factor alpha correlate with peritoneal adhesion grades in humans after major abdominal surgery[J]. Am Surg, 1998, 64(8): 734-737.
19.	Riese J, Denzel C, Mehler C, et al. The diminished postoperative capacity of blood leukocytes to produce IL-6 is associated with high concentrations of IL-6 in the circulation[J]. Cytokine, 2000, 12(5): 531-534.
20.	Saba AA, Kaidi AA, Godziachvili V, et al. Effects of interleukin-6 and its neutralizing antibodies on peritoneal adhesion formation and wound healing[J]. Am Surg, 1996, 62(7): 569-572.
21.	Ambler DR, Fletcher NM, Diamond MP, et al. Effects of hypoxia on the expression of inflammatory markers IL-6 and TNF-a in human normal peritoneal and adhesion fibroblasts[J]. Syst Biol Reprod Med, 2012, 58(6): 324-329.
22.	王春霞, 侯连兵, 刘世霆.常通口服液对术后肠粘连大鼠血清细胞因子的影响[J].南方医科大学学报, 2005, 25(6): 601-604.

1. Tokita Y, Yuzurihara M, Satoh K, et al. The cholinergic nervous system plays an important role in rat postoperative intestinal adhesion[J]. Surgery, 2008, 143(2): 226-232.
2. Hellebrekers BW, Kooistra T. Pathogenesis of postoperative adhesion formation[J]. Br J Surg, 2011, 98(11): 1503-1516.
3. 李焱燚, 王永刚, 刘学源.β-七叶皂苷钠的研究进展[J].神经病学与神经康复学杂志, 2009, 6(2): 147-150.
4. Li X, Chen GP, Li L, et al. Dual effects of sodium aescinate on vascular tension in rat thoracic aorta[J]. Microvasc Res, 2010, 79(1): 63-69.
5. Wang YK, Han J, Xiong WJ, et al. Evaluation of in vivo antioxidant and immunity enhancing activities of sodium aescinate injection liquid[J]. Molecules, 2012, 17(9): 10267-10275.
6. 李冬梅, 徐丽, 曹军平, 等.七叶皂苷钠预防术后肠粘连的实验和临床研究[J].世界中医药, 2013, 8(10): 1164-1165, 1168.
7. Phillips RK, Dudley HA. The effect of tetracycline lavage and trauma on visceral and parietal peritoneal ultrastructure and adhesion formation[J]. Br J Surg, 1984, 71(7): 537-539.
8. 王春霞.常通口服液对炎症因子参与大鼠肠粘连的影响[J].中国药师, 2005, 8(7): 535-537.
9. Imudia AN, Kumar S, Saed GM, et al. Pathogenesis of intraabdominal and pelvic adhesion development[J]. Semin Reprod Med, 2008, 26(4): 289-297.
10. Ozoğul Y, Baykal A, Onat D, et al. An experimental study of the effect of aprotinin on intestinal adhesion formation[J]. Am J Surg, 1998, 175(2): 137-141.
11. 隋新兵, 张勤, 裘华森, 等.丹参多酚酸盐预防大鼠术后肠粘连的机制[J].中西医结合学报, 2007, 5(5): 521-525.
12. Kosaka H, Yoshimoto T, Yoshimoto T, et al. Interferon-gamma is a therapeutic target molecule for prevention of postoperative adhesion formation[J]. Nat Med, 2008, 14(4): 437-441.
13. Franke A, Lante W, Kurig E, et al. Postoperative PMBC-derived IL-6 and TNF-alpha-release is uninfluenced by IL-12-mediated restoration of IFN-gamma synthesis[J]. Scand Cardiovasc J, 2009, 43(2): 136-144.
14. Kawashima R, Kawamura YI, Oshio T, et al. Comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy[J]. J Immunoassay Immunochem, 2012, 33(3): 291-301.
15. Kaidi AA, Gurchumelidze T, Nazzal M, et al. Tumor necrosis factor-α: a marker for peritoneal adhesion formation[J]. J Surg Res, 1995, 58(5): 516-518.
16. Kurukahvecioglu O, Koksal H, Gulbahar O, et al. Infliximab "TNF-alpha antagonist" decreases intraabdominal adhesions[J]. Saudi Med J, 2007, 28(12): 1830-1835.
17. Bessler H, Shavit Y, Mayburd E, et al. Postoperative pain, morphine consumption, and genetic polymorphism of IL-1beta and IL-1 receptor antagonist[J]. Neurosci Lett, 2006, 8(4): 190.
18. Saba AA, Godziachvili V, Mavani AK, et al. Serum levels of interleukin 1 and tumor necrosis factor alpha correlate with peritoneal adhesion grades in humans after major abdominal surgery[J]. Am Surg, 1998, 64(8): 734-737.
19. Riese J, Denzel C, Mehler C, et al. The diminished postoperative capacity of blood leukocytes to produce IL-6 is associated with high concentrations of IL-6 in the circulation[J]. Cytokine, 2000, 12(5): 531-534.
20. Saba AA, Kaidi AA, Godziachvili V, et al. Effects of interleukin-6 and its neutralizing antibodies on peritoneal adhesion formation and wound healing[J]. Am Surg, 1996, 62(7): 569-572.
21. Ambler DR, Fletcher NM, Diamond MP, et al. Effects of hypoxia on the expression of inflammatory markers IL-6 and TNF-a in human normal peritoneal and adhesion fibroblasts[J]. Syst Biol Reprod Med, 2012, 58(6): 324-329.
22. 王春霞, 侯连兵, 刘世霆.常通口服液对术后肠粘连大鼠血清细胞因子的影响[J].南方医科大学学报, 2005, 25(6): 601-604.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Mechanism of Sodium Aescinate in Preventing Postoperative Intestinal Adhesion in Rats

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