Expression of miR21 in Rectal Cancer of Different Tumor Stages_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YANGYun ¹ , PENGWei ² , WANGXiao-dong ¹ , TANGTian ² , DUANBao-feng ³ , XIALin ¹ ,  SHUYe ¹

1. Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China;
2. West China Medical School, Sichuan University, Chengdu 610041, Sichuan Province, China;
3. Department of Oncology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China;

Corresponding author：

SHUYe, Email: sy999222@163.com

Keywords：

MicroRNA; miR21; Rectal cancer; N stage; Dukes stage

DOI：

10.7507/1007-9424.20140234

Video：

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Objective To quantitate expression of miR21 in rectal cancer of different tumor stages and discuss their significances. Method The expression of miR21 was detected and quantitated in the rectal cancer tissues and corresponding adjacent normal tissues of 40 patients with rectal cancer in this hospital from August 2012 to October 2012 by Taq Man microRNA assay. Results The significant overexpression of miR21 was observed in the rectal cancer tissues (4.122±1.973 versus 1.825±0.661, P=0.000)as compared with the corresponding adjacent normal tissues. The expressions of miR21 in the rectal cancer tissues of N₁-N₂ and Dukes C-D stages were significantly higher than those in the rectal cancer tissues of N₀(4.852±2.344 versus 3.391±1.171, P=0.019)and Dukes A-B stage(4.787±2.304 versus 3.386±1.203, P=0.021). From N₀ to N₂ stage, the expression of miR21 increased progressively in the rectal cancer, and the expression in the rectal cancer tissues of N₂ stage was significantly higher than that in the N₀ stage(5.556±1.500 versus 3.391±1.171, P=0.010). And receiver operating characteristics curve analysis showed that miR21 could discriminate N 0 stage from N₁-N₂ stage with a 0.698 area under curve(AUC), 50.0% sensitivity and 90.0% specificity, Dukes C-D stage from A-B stage with a 0.689 AUC, 42.9% sensitivity and 94.7% specificity. Conclusion miR21 appears to have a potential correlation with N and Dukes stages of rectal cancer, which cautiously and optimistically suggests that it could be a potential novel biomarker for predicting tumor stage preoperatively.

Citation： YANGYun, PENGWei, WANGXiao-dong, TANGTian, DUANBao-feng, XIALin, SHUYe. Expression of miR21 in Rectal Cancer of Different Tumor Stages. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(8): 971-975. doi: 10.7507/1007-9424.20140234 Copy

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1. Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013[J]. CA Cancer J Clin, 2013, 63(1): 11-30.
2. Figueredo A, Coombes ME, Mukherjee S. Adjuvant therapy for completely resected stageⅡcolon cancer[J]. Cochrane Database Syst Rev, 2008(3): CD005390.
3. Esquela-Kerscher A, Slack FJ. Oncomirs-microRNAs with a role in cancer[J]. Nat Rev Cancer, 2006, 6(4): 259-269.
4. He L, Hannon GJ. MicroRNAs: small RNAs with a big role in gene regulation[J]. Nat Rev Genet, 2004, 5(7): 522-531.
5. Lai EC. Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation[J]. Nat Genet, 2002, 30(4): 363-364.
6. Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1): 15-20.
7. Lim LP, Lau NC, Garrett-Engele P, et al. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs[J]. Nature, 2005, 433(727): 769-773.
8. Huang Q, Gumireddy K, Schrier M, et al. The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis[J]. Nat Cell Biol, 2008, 10(2): 202-210.
9. Iwaya T, Yokobori T, Nishida N, et al. Downregulation of miR-144 is associated with colorectal cancer progression via activation of mTOR signaling pathway[J]. Carcinogenesis, 2012, 33(12): 2391-2397.
10. Zhang GJ, Xiao HX, Tian HP, et al. Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression[J]. Int J Mol Med, 2013, 31(6): 1375-1380.
11. Chiang Y, Song Y, Wang Z, et al. microRNA-192, -194 and-215 are frequently downregulated in colorectal cancer[J]. Exp Ther Med, 2012, 3(3): 560-566.
12. Yan LX, Huang XF, Shao Q, et al. MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis[J]. RNA, 2008, 14(11): 2348-2360.
13. Li Y, Li W, Ouyang Q, et al. Detection of lung cancer with blood microRNA-21 expression levels in Chinese population[J]. Oncol Lett, 2011, 2(5): 991-994.
14. Li L, Zhou L, Li Y, et al. MicroRNA-21 stimulates gastric cancer growth and invasion by inhibiting the tumor suppressor effects of programmed cell death protein 4 and phosphatase and tensin homolog[J]. J BUON, 2014, 19(1): 228-236.
15. Zhang J, Yang Y, Liu Y, et al. MicroRNA-21 regulates biological behaviors in papillary thyroid carcinoma by targeting programmed cell death 4[J]. J Surg Res, 2014, 189(1): 68-74.
16. Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers[J]. Nature, 2005, 435(743): 834-838.
17. Waldman SA, Terzic A. MicroRNA signatures as diagnostic and therapeutic targets[J]. Clin Chem, 2008, 54(6): 943-944.
18. Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes[J]. Genome Biol, 2002, 3(7): RESEARCH0034.
19. Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12): 2893-2917.
20. 彭伟, 杨韵, 刘韵霄, 等. microRNA在结直肠癌中的研究及应用进展[J].中国普外基础与临床杂志, 2013, 20(6): 691-696.
21. Karantanas A, Yarmenitis S, Papanikolaou N, et al. Preoperative imaging staging of rectal cancer[J]. Dig Dis, 2007, 25(1): 20-32.
22. 李丹, 郭丽英. microRNA与乳腺癌浸润转移关系的研究进展[J].中国普外基础与临床杂志, 2014, 21(5): 654-657.
23. Asangani IA, Rasheed SA, Nikolova DA, et al. MicroRNA-21(miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer[J]. Oncogene, 2008, 27(15): 2128-2136.
24. Wang Q, Zhu J, Zhang Y, et al. Down-regulation of programmed cell death 4 lead to epithelial to mesenchymal transition and promotes metastasis in mice[J]. Eur J Cancer, 2013, 49(7): 1761-1770.
25. Slaby O, Svoboda M, Fabian P, et al. Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer[J]. Oncology, 2007, 72(5/6): 397-402.
26. Schetter AJ, Leung SY, Sohn JJ, et al. MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma[J]. JAMA, 2008, 299(4): 425-436.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of miR21 in Rectal Cancer of Different Tumor Stages

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