Protective Effect of Controlled Reperfusion with Xuebijing Injection on Ischemia-Reperfusion Injury of Rabbit Hind Limb_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WANGGang ,  ZHONGHong-shan

Department of Invasive Technology, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China;

Corresponding author：

ZHONGHong-shan, Email: 334864946@qq.com

Keywords：

Ischemia reperfusion; Controlled reperfusion; Hind limb; Xuebijing injection

DOI：

10.7507/1007-9424.20150149

Video：

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Objective To discuss the protection effect of controlled reperfusion with Xuebijing injection on ischemia-reperfusion injury of rabbit hind limb, and provide a theoretical basis for prevention and treatment of limb ischemia reperfusion injury in clinical. Methods The big ear rabbit model of hind limb ischemia-reperfusion injury (ischemia 2 h, reperfusion 4 h) was made. Thirty healthy big ear rabbits were randomly divided into three groups: Xuebijing perfusion group (n=10): Xuebijing injection was given before reperfusion; Saline control group (n=10): surgical procedure with Xuebijing perfusion group, saline infusion was given before reperfusion; sham operation group (n=10): surgical procedure with Xuebijing perfusion group, ischemia and reperfusion was not performed. The activity of serum superoxide dismutase (SOD) and content of malondialdehyde (MDA) were detected. The ratio of blood flow (rBF) and ratio of blood volume (rBV) were tested. Results ①Compared with the levels before operation, the activity of SOD and content of MDA had no significant differences after operation in the sham operation group (P > 0.05), the activity of SOD was obviously increased and the content of MDA was obviously decreased after reperfusion in the Xuebijing perfusion group (P < 0.01). Compared with the saline control group, the activity of SOD was obviously increased and the content of MDA was obviously decreased in the Xuebijing perfusion group.②Compared with the sham operation group, the rBF and rBV were obviously decreased in the Xuebijing perfusion group and the saline control group (P < 0.05); Compared with the saline control group, the rBF and rBV were obviously increased in the Xuebijing perfusion group (P < 0.05). Conclusion Controlled reperfusion with Xuebijing injection could increase SOD activity in serum, reduce MDA content, it has a protective effect on hind limb ischemia-reperfusion injury in rabbits, and could effectively improve perfusion of hind limb.

Citation： WANGGang, ZHONGHong-shan. Protective Effect of Controlled Reperfusion with Xuebijing Injection on Ischemia-Reperfusion Injury of Rabbit Hind Limb. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(5): 560-564. doi: 10.7507/1007-9424.20150149 Copy

1.	Hua HT, Albadawi H, Entabi F, et al. Polyadenosine diphosphate-ribose polymerase inhibition modulates skeletal muscle injury following ischemia reperfusion. Arch Surg, 2005, 140(4):344-351.
2.	Sako H, Hadama T, Miyamoto S, et al. Limb ischemia and reperfusion during abdominal aortic aneurysm surgery. Surg Today, 2004, 34(10):832-836.
3.	Ben-Ari Z, Issan Y, Katz Y, et al. Induction of heme oxygenase-1 protects mouse liver from apoptotic ischemia/reperfusion injury. Apoptosis, 2013, 18(5):547-555.
4.	Beyersdorf F, Schlensak C. Controlled reperfusion, after acute and persistent limb ischemia. Semin Vasc Surg, 2009, 22(1):52-57.
5.	雪琳. SIRS和MODS防治新对策的实验研究-血必净的药效学观察.中国危重病急救医学, 1997, 9(12):720-722.
6.	石臣磊, 秦华东, 丁超.依达拉奉对肝缺血再灌注损伤逆转作用的研究.中国普外基础与临床杂志, 2011, 18(8):887-889.
7.	McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med, 1985, 312(3):159-163.
8.	Menger MD, Steiner D, Messmer K. Microvascular ischemiareperfusion injury in striated muscle:significance of" no reflow". Am J Physiol, 1992, 263 (6 Pt 2):H1892-H1900.
9.	Duehrkop C, Banz Y, Spirig R, et al. C1 esterase inhibitor reduces lower extremity ischemia/reperfusion injury and associated lung damage. PLoS One, 2013, 8(8):e72059.
10.	Ay Y, Kara I, Aydin C, et al. Effects of ischemic preconditioning and iloprost on myocardial ischemia-reperfusion damage in rats. Int J Clin Exp Med, 2013, 6(7):516-523.
11.	Gyurkovics E, Aranyi P, Stangl R, et al. Postconditioning of the lower limb-protection against the reperfusion syndrome. J Surg Res, 2011, 169(1):139-147.
12.	Kalkan E, Keskin F, Kaya B, et al. Effects of iloprost and piracetam in spinal cord ischemia-reperfusion injury in the rabbit. Spinal Cord, 2011, 49(1):81-86.
13.	Eliason JL, Wakefield TW. Metabolic consequences of acute limb ischemia and their clinical implications. Semin Vasc Surg, 2009, 22(1):29-33.
14.	Boros P, Bromberg JS. New cellular and molecular immune pathways in ischemia/reperfusion injury. Am J Transplant, 2006, 6(4):652-658.
15.	Wang WZ, Fang XH, Stephenson LL, et al. Melatonin attenuates I/R-induced mitochondrial dysfunction in skeletal muscle. J Surg Res, 2011, 171(1):108-113.
16.	Avci G, Kadioglu H, Sehirli AO, et al. Curcumin protects against ischemia/reperfusion injury in rat skeletal muscle. J Surg Res, 2012, 172(1):e39-e46.
17.	McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem, 1969, 244(22):6049-6055.
18.	Wang Y, Shen J, Xiong X, et al. Remote ischemic preconditioning protects against liver ischemia-reperfusion injury via heme oxygenase-1-induced autophagy. PLoS One, 2014, 9(6):e98834.
19.	Eberlin KR, McCormack MC, Nguyen JT, et al. Ischemic preconditioning of skeletal muscle mitigates remote injury and mortality. J Surg Res, 2008, 148(1):24-30.
20.	谭萍, 张凌云, 张国欣, 等.参附注射液对大鼠肢体缺血再灌注后肝脏损伤的保护作用.中国普外基础与临床杂志, 2014, 21(8):960-964.
21.	高红梅, 常文秀, 曹书华."血必净"注射液对内毒素刺激的内皮细胞的影响.中国急救医学, 2005, 25(6):437-438.
22.	张庆洋, 孙建平, 王永军, 等.血必净对严重烧伤患者早期全身炎症反应的影响.中华烧伤杂志, 2011, 27(3):183-184.
23.	归咏刚, 姚咏明, 柴艳芬.血必净注射液对脓毒症大鼠白细胞介素-6表达及血小板的影响.中国急救复苏与灾害医学杂志, 2009, 4(1):13-16.
24.	何国鑫, 胡慧敏, 罗文朝, 等.血必净注射液对急性肺损伤大鼠氧自由基变化的影响.实用医学杂志, 2009, 25(12):1916-1919.
25.	张良, 代维, 高志明, 等.血必净注射液对兔肢体缺血再灌注损伤的治疗作用研究.创伤外科杂志, 2013, 15(1):63-67.

1. Hua HT, Albadawi H, Entabi F, et al. Polyadenosine diphosphate-ribose polymerase inhibition modulates skeletal muscle injury following ischemia reperfusion. Arch Surg, 2005, 140(4):344-351.
2. Sako H, Hadama T, Miyamoto S, et al. Limb ischemia and reperfusion during abdominal aortic aneurysm surgery. Surg Today, 2004, 34(10):832-836.
3. Ben-Ari Z, Issan Y, Katz Y, et al. Induction of heme oxygenase-1 protects mouse liver from apoptotic ischemia/reperfusion injury. Apoptosis, 2013, 18(5):547-555.
4. Beyersdorf F, Schlensak C. Controlled reperfusion, after acute and persistent limb ischemia. Semin Vasc Surg, 2009, 22(1):52-57.
5. 雪琳. SIRS和MODS防治新对策的实验研究-血必净的药效学观察.中国危重病急救医学, 1997, 9(12):720-722.
6. 石臣磊, 秦华东, 丁超.依达拉奉对肝缺血再灌注损伤逆转作用的研究.中国普外基础与临床杂志, 2011, 18(8):887-889.
7. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med, 1985, 312(3):159-163.
8. Menger MD, Steiner D, Messmer K. Microvascular ischemiareperfusion injury in striated muscle:significance of" no reflow". Am J Physiol, 1992, 263 (6 Pt 2):H1892-H1900.
9. Duehrkop C, Banz Y, Spirig R, et al. C1 esterase inhibitor reduces lower extremity ischemia/reperfusion injury and associated lung damage. PLoS One, 2013, 8(8):e72059.
10. Ay Y, Kara I, Aydin C, et al. Effects of ischemic preconditioning and iloprost on myocardial ischemia-reperfusion damage in rats. Int J Clin Exp Med, 2013, 6(7):516-523.
11. Gyurkovics E, Aranyi P, Stangl R, et al. Postconditioning of the lower limb-protection against the reperfusion syndrome. J Surg Res, 2011, 169(1):139-147.
12. Kalkan E, Keskin F, Kaya B, et al. Effects of iloprost and piracetam in spinal cord ischemia-reperfusion injury in the rabbit. Spinal Cord, 2011, 49(1):81-86.
13. Eliason JL, Wakefield TW. Metabolic consequences of acute limb ischemia and their clinical implications. Semin Vasc Surg, 2009, 22(1):29-33.
14. Boros P, Bromberg JS. New cellular and molecular immune pathways in ischemia/reperfusion injury. Am J Transplant, 2006, 6(4):652-658.
15. Wang WZ, Fang XH, Stephenson LL, et al. Melatonin attenuates I/R-induced mitochondrial dysfunction in skeletal muscle. J Surg Res, 2011, 171(1):108-113.
16. Avci G, Kadioglu H, Sehirli AO, et al. Curcumin protects against ischemia/reperfusion injury in rat skeletal muscle. J Surg Res, 2012, 172(1):e39-e46.
17. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem, 1969, 244(22):6049-6055.
18. Wang Y, Shen J, Xiong X, et al. Remote ischemic preconditioning protects against liver ischemia-reperfusion injury via heme oxygenase-1-induced autophagy. PLoS One, 2014, 9(6):e98834.
19. Eberlin KR, McCormack MC, Nguyen JT, et al. Ischemic preconditioning of skeletal muscle mitigates remote injury and mortality. J Surg Res, 2008, 148(1):24-30.
20. 谭萍, 张凌云, 张国欣, 等.参附注射液对大鼠肢体缺血再灌注后肝脏损伤的保护作用.中国普外基础与临床杂志, 2014, 21(8):960-964.
21. 高红梅, 常文秀, 曹书华."血必净"注射液对内毒素刺激的内皮细胞的影响.中国急救医学, 2005, 25(6):437-438.
22. 张庆洋, 孙建平, 王永军, 等.血必净对严重烧伤患者早期全身炎症反应的影响.中华烧伤杂志, 2011, 27(3):183-184.
23. 归咏刚, 姚咏明, 柴艳芬.血必净注射液对脓毒症大鼠白细胞介素-6表达及血小板的影响.中国急救复苏与灾害医学杂志, 2009, 4(1):13-16.
24. 何国鑫, 胡慧敏, 罗文朝, 等.血必净注射液对急性肺损伤大鼠氧自由基变化的影响.实用医学杂志, 2009, 25(12):1916-1919.
25. 张良, 代维, 高志明, 等.血必净注射液对兔肢体缺血再灌注损伤的治疗作用研究.创伤外科杂志, 2013, 15(1):63-67.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Effect of Controlled Reperfusion with Xuebijing Injection on Ischemia-Reperfusion Injury of Rabbit Hind Limb

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