Changes in Level of Myelin Basic Protein,TNF-α and IL-6, and Their Correlative Study in Experimental Rat of Pancreatic Encephalopathy_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HUANGBo-ru ,  ZHAOHai-ping ,  HUWen-xiu

Department of General Surgery, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China;

Corresponding author：

ZHAOHai-ping, Email: zhaohaiping007@aliyun.com; HUWen-xiu, Email: 574752493@qq.com

Keywords：

Pancreatic encephalopathy; Myelin basic protein; Tumor necrosis factor-α; Interleukin

DOI：

10.7507/1007-9424.20150211

Video：

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Objective To discuss the changes of myelin basic protein (MBP), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in serum and cerebrospinal fluid of experimental pancreatic encephalopathy rat model, analyze the relationship between each factor and the occurrence and development of pancreatic encephalopathy, and to provide the experimental basis for clinical diagnosis and treatment of pancreatic encephalopathy. Methods Selecting 40 SD rats were randomly divided into sham operation group (SO group, n=10) and pancreatic encephalopathy group (PE group, n=30), respectively by the duodenal papilla retrograde pancreatic puncture injection of saline solution or 5% sodium taurocholic acid induced rat pancreatic encephalopathy model were set up. The rats in SO group were sacrificed on 1 d, and the PE group were sacrificed ten rats on 1 d, 3 d, and 7 d, respectively after surgery. The brain and pancreatic tissues of rats in each group were taken to observe the pathological changes of the rats and the brain white blood cells within microvessels gathered and coanda phenomenon. The water content of brain tissues, and the contents of MBP, TNF-α and TL-6 in serum and cerebrospinal fluid were detected. Results The changes of brain nerve cell edema and nerve fiber demyelination were obvious in PE group rats after surgery with the extension of time. The contents of MBP, TNF-α and TL-6 in serum and cerebrospinal fluid on 1 d, 3 d, and 7 d after surgery in PE group were significantly higher than that SO group (P<0.05), and gradually increased with the extension of time. But by two two compared, the change trend of the above three indicators were different. Conclusions MBP, TNF, and IL-6 on the occurrence and development of brain damage of pancreatic encephalopathy play a synergistic effect. To detecte the MBP, TNF-a, and IL-6 content in blood and cerebrospinal fluid could be diagnosed and evaluated the pancreatic encephalopathy.

Citation： HUANGBo-ru, ZHAOHai-ping, HUWen-xiu. Changes in Level of Myelin Basic Protein,TNF-α and IL-6, and Their Correlative Study in Experimental Rat of Pancreatic Encephalopathy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(7): 816-821. doi: 10.7507/1007-9424.20150211 Copy

1.	马代全, 黄桂蛟, 田道蓉, 等. 急性重症胰腺炎合并胰性脑病的临床诊治. 西部医学, 2012, 24(7):1320-1321, 1324.
2.	王艳茹, 麻继臣, 张晓岚, 等. 胰性脑病的诊治进展. 医学综述, 2010, 16(4):595-598.
3.	Sharma V, Sharma R, Rana SS, et al. Pancreatic encephalopathy:an unusual cause of asterixis. JOP, 2014, 15(4):383-384.
4.	江少娜. 胰性脑病的研究现状. 实用心脑肺血管病杂志, 2012, 20(2):373-375.
5.	孔雷, 韩天权, 汤耀卿, 等. 对胰性脑病病因和发病机制的认识. 中国现代普通外科进展, 2002, 5(2):68-70.
6.	Qiu F, Lu XS, Huang YK. Protective effect of low-molecular-weight heparin on pancreatic encephalopathy in severe acute pancreatic rats. Inflamm Res, 2012, 61(11):1203-1209.
7.	Hornik A, Rodriguez Porcel FJ, Agha C, et al. Central and extrapontine myelinolysis affecting the brain and spinal cord. An unusual presentation of pancreatic encephalopathy. Front Neurol, 2012, 3:135.
8.	Mameli G, Cossu D, Cocco E, et al. Epstein-Barr virus and Mycobacterium avium subsp. paratuberculosis peptides are cross recognized by anti-myelin basic protein antibodies in multiple sclerosis patients. J Neuroimmunol, 2014, 270(1-2):51-55.
9.	刘小丰, 钱祝银, 苗毅, 等. 中枢脱髓鞘病变的免疫机制与胰性脑病. 中国现代普通外科进展, 2004, 7(3):129-130.
10.	覃惠洵, 刘琳琳, 郝洪军, 等. OCB、抗MBP抗体及抗MOG抗体在中枢神经系统炎性脱髓鞘疾病诊断中的意义. 中国实用神经疾病杂志, 2014, 17(8):11-13.
11.	Hedegaard CJ, Chen N, Sellebjerg F, et al. Autoantibodies to myelin basic protein (MBP) in healthy individuals and in patients with multiple sclerosis:a role in regulating cytokine responses to MBP. Immunology, 2009, 128(1 Suppl):e451-e461.
12.	郭遂成, 杨敬端. 胰性脑病24例临床治疗观察. 中国社区医师(医学专业), 2013, 15(9):40.
13.	侯杰. 重症急性胰腺炎并胰性脑病的临床分析. 中国医药指南, 2013, 11(11):433-434.
14.	周志军. 胰性脑病一例. 腹部外科, 2013, 26(3):214.
15.	周建峰. 86例重症急性胰腺炎临床预后相关分析. 医药前沿, 2012, 29:100-101.
16.	Cosmi L, Liotta F, Maggi E, et al. Th17 and non-classic Th1 cells in chronic inflammatory disorders:two sides of the same coin. J Int Arch Allergy Immunol, 2014, 164(3):171-177.
17.	Hidaka Y, Inaba Y, Matsuda K, et al. Cytokine production profiles in chronic relapsing-remitting experimental autoimmune encephalomyelitis:IFN-gamma and TNF-alpha are important participants in the first attack but not in the relapse. J Neurol Sci. 2014, 340(1-2):117-122.
18.	Belogurov A Jr, Kudriaeva A, Kuzina E, et al. Multiple sclerosis autoantigen myelin basic protein escapes control by ubiquitination during proteasomal degradation. J Biol Chem, 2014, 289(25):17758-17766.
19.	Yamasaki R. The role of microglia in inflammatory demyelination lesion in the central nervous system. Rinsho Shinkeigaku, 2014, 54 (12):981-983.
20.	Kersh AE, Edwards LJ, Evavold BD, et al. Progression of relapsing-remitting demyelinating disease does not require increased TCR affinity or epitope spread. J Immunol, 2014, 193(9):4429-4438.
21.	Bsibsi M, Peferoen LA, Holtman IR, et al. Demyelination during multiple sclerosis is associated with combined activation of microglia/macrophages by IFN-gamma and alpha B-crystallin. Acta Neuropathol, 2014, 128(2):215-229.
22.	Kaltsonoudis E, Zikou AK, Voulgari PV, et al. Neurological adverse events in patients receiving anti-TNF therapy:a prospective imaging and electrophysiological study. Arthritis Res Ther, 2014, 16(3):R125.
23.	Ramirez-Ramirez V, Macias-Islas MA, Ortiz GG, et al. Efficacy of fish oil on serum of TNF alpha, IL-1 beta, and IL-6 oxidative stress markers in multiple sclerosis treated with interferon beta-1b. Oxid Med Cell Longev, 2013, 2013:709493.
24.	Li J, Yang WJ, Huang LM, et al. Immunomodulatory therapies for acute pancreatitis. World J Gastroenterol, 2014, 20(45):16935-16947.
25.	Hori T, Uemoto S, Walden LB, et al. Matrix metalloproteinase-9 as a therapeutic target for the progression of fulminant liver failure with hepatic encephalopathy:A pilot study in mice. Hepatol Res, 2014, 44(6):651-662.
26.	Clancy-Thompson E, King LK, Nunnley LD, et al. Peptide vaccination in Montanide adjuvant induces and GM-CSF increases CXCR3 and cutaneous lymphocyte antigen expression by tumor antigen-specific CD8 T cells. Cancer Immunol Res, 2013, 1(5):332-339.
27.	Doronin VB, Parkhomenko TA, Castellazzi M, et al. Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis. PLoS One. 2014, 9(9):e107807.
28.	Kaltsonoudis E, Zikou A K, Voulgari P V, et al. Neurological adverse events in patients receiving anti-TNF therapy:a prospective imaging and electrophysiological study. Arthritis Res Ther. 2014, 16(3):R125.
29.	Ferreira T B, Hygino J, Barros P O, et al. Endogenous interleukin-6 amplifies interleukin-17 production and corticoid-resistance in peripheral T cells from patients with multiple sclerosis. Immunology, 2014, 143(4):560-568.
30.	Trinschek B, Luessi F, Haas J, et al. Kinetics of IL-6 production defines T effector cell responsiveness to regulatory T cells in multiple sclerosis. PLoS One, 2013, 8(10):e77634.
31.	Wullschleger A, Kapina V, Molnarfi N, et al. Cerebrospinal fluid interleukin-6 in central nervous system inflammatory diseases. PLoS One, 2013, 8(8):e72399.

1. 马代全, 黄桂蛟, 田道蓉, 等. 急性重症胰腺炎合并胰性脑病的临床诊治. 西部医学, 2012, 24(7):1320-1321, 1324.
2. 王艳茹, 麻继臣, 张晓岚, 等. 胰性脑病的诊治进展. 医学综述, 2010, 16(4):595-598.
3. Sharma V, Sharma R, Rana SS, et al. Pancreatic encephalopathy:an unusual cause of asterixis. JOP, 2014, 15(4):383-384.
4. 江少娜. 胰性脑病的研究现状. 实用心脑肺血管病杂志, 2012, 20(2):373-375.
5. 孔雷, 韩天权, 汤耀卿, 等. 对胰性脑病病因和发病机制的认识. 中国现代普通外科进展, 2002, 5(2):68-70.
6. Qiu F, Lu XS, Huang YK. Protective effect of low-molecular-weight heparin on pancreatic encephalopathy in severe acute pancreatic rats. Inflamm Res, 2012, 61(11):1203-1209.
7. Hornik A, Rodriguez Porcel FJ, Agha C, et al. Central and extrapontine myelinolysis affecting the brain and spinal cord. An unusual presentation of pancreatic encephalopathy. Front Neurol, 2012, 3:135.
8. Mameli G, Cossu D, Cocco E, et al. Epstein-Barr virus and Mycobacterium avium subsp. paratuberculosis peptides are cross recognized by anti-myelin basic protein antibodies in multiple sclerosis patients. J Neuroimmunol, 2014, 270(1-2):51-55.
9. 刘小丰, 钱祝银, 苗毅, 等. 中枢脱髓鞘病变的免疫机制与胰性脑病. 中国现代普通外科进展, 2004, 7(3):129-130.
10. 覃惠洵, 刘琳琳, 郝洪军, 等. OCB、抗MBP抗体及抗MOG抗体在中枢神经系统炎性脱髓鞘疾病诊断中的意义. 中国实用神经疾病杂志, 2014, 17(8):11-13.
11. Hedegaard CJ, Chen N, Sellebjerg F, et al. Autoantibodies to myelin basic protein (MBP) in healthy individuals and in patients with multiple sclerosis:a role in regulating cytokine responses to MBP. Immunology, 2009, 128(1 Suppl):e451-e461.
12. 郭遂成, 杨敬端. 胰性脑病24例临床治疗观察. 中国社区医师(医学专业), 2013, 15(9):40.
13. 侯杰. 重症急性胰腺炎并胰性脑病的临床分析. 中国医药指南, 2013, 11(11):433-434.
14. 周志军. 胰性脑病一例. 腹部外科, 2013, 26(3):214.
15. 周建峰. 86例重症急性胰腺炎临床预后相关分析. 医药前沿, 2012, 29:100-101.
16. Cosmi L, Liotta F, Maggi E, et al. Th17 and non-classic Th1 cells in chronic inflammatory disorders:two sides of the same coin. J Int Arch Allergy Immunol, 2014, 164(3):171-177.
17. Hidaka Y, Inaba Y, Matsuda K, et al. Cytokine production profiles in chronic relapsing-remitting experimental autoimmune encephalomyelitis:IFN-gamma and TNF-alpha are important participants in the first attack but not in the relapse. J Neurol Sci. 2014, 340(1-2):117-122.
18. Belogurov A Jr, Kudriaeva A, Kuzina E, et al. Multiple sclerosis autoantigen myelin basic protein escapes control by ubiquitination during proteasomal degradation. J Biol Chem, 2014, 289(25):17758-17766.
19. Yamasaki R. The role of microglia in inflammatory demyelination lesion in the central nervous system. Rinsho Shinkeigaku, 2014, 54 (12):981-983.
20. Kersh AE, Edwards LJ, Evavold BD, et al. Progression of relapsing-remitting demyelinating disease does not require increased TCR affinity or epitope spread. J Immunol, 2014, 193(9):4429-4438.
21. Bsibsi M, Peferoen LA, Holtman IR, et al. Demyelination during multiple sclerosis is associated with combined activation of microglia/macrophages by IFN-gamma and alpha B-crystallin. Acta Neuropathol, 2014, 128(2):215-229.
22. Kaltsonoudis E, Zikou AK, Voulgari PV, et al. Neurological adverse events in patients receiving anti-TNF therapy:a prospective imaging and electrophysiological study. Arthritis Res Ther, 2014, 16(3):R125.
23. Ramirez-Ramirez V, Macias-Islas MA, Ortiz GG, et al. Efficacy of fish oil on serum of TNF alpha, IL-1 beta, and IL-6 oxidative stress markers in multiple sclerosis treated with interferon beta-1b. Oxid Med Cell Longev, 2013, 2013:709493.
24. Li J, Yang WJ, Huang LM, et al. Immunomodulatory therapies for acute pancreatitis. World J Gastroenterol, 2014, 20(45):16935-16947.
25. Hori T, Uemoto S, Walden LB, et al. Matrix metalloproteinase-9 as a therapeutic target for the progression of fulminant liver failure with hepatic encephalopathy:A pilot study in mice. Hepatol Res, 2014, 44(6):651-662.
26. Clancy-Thompson E, King LK, Nunnley LD, et al. Peptide vaccination in Montanide adjuvant induces and GM-CSF increases CXCR3 and cutaneous lymphocyte antigen expression by tumor antigen-specific CD8 T cells. Cancer Immunol Res, 2013, 1(5):332-339.
27. Doronin VB, Parkhomenko TA, Castellazzi M, et al. Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis. PLoS One. 2014, 9(9):e107807.
28. Kaltsonoudis E, Zikou A K, Voulgari P V, et al. Neurological adverse events in patients receiving anti-TNF therapy:a prospective imaging and electrophysiological study. Arthritis Res Ther. 2014, 16(3):R125.
29. Ferreira T B, Hygino J, Barros P O, et al. Endogenous interleukin-6 amplifies interleukin-17 production and corticoid-resistance in peripheral T cells from patients with multiple sclerosis. Immunology, 2014, 143(4):560-568.
30. Trinschek B, Luessi F, Haas J, et al. Kinetics of IL-6 production defines T effector cell responsiveness to regulatory T cells in multiple sclerosis. PLoS One, 2013, 8(10):e77634.
31. Wullschleger A, Kapina V, Molnarfi N, et al. Cerebrospinal fluid interleukin-6 in central nervous system inflammatory diseases. PLoS One, 2013, 8(8):e72399.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Changes in Level of Myelin Basic Protein,TNF-α and IL-6, and Their Correlative Study in Experimental Rat of Pancreatic Encephalopathy

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