• Department of Biliary Surgery, West China Hospital, Sichuan University. Chengdu 610041, Sichuan Province, China;
ChengNan-sheng, Email: chengnansheng2014@126.com
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Objective To investigate the effect of peroxisome proliferator-activated receptorγ(PPARγ) agonist on bile duct fibrosis induced by transforming growth factor-β1 (TGF-β1). Methods Primary cultures of bile duct fibroblast were treated with different concentrations of pioglitazone (PGZ) or 15-deoxy-Δ12, 14-prostaglandin J2 (15d-PGJ2) and then treated with TGF-β1. The mRNA levels of collagenⅠ(COLⅠ)、fibronectin (FN)、α-smooth muscle actin (α-SMA) and connective tissue growth factor (CTGF) were determined by RT-PCR. Results PGZ and 15-d-PGJ2 could down regulated the mRNA levels of COLⅠ, FN, α-SMA and CTGF induced by TGF-β1 (3 ng/mL). The same concentration of 15d-PGJ2 was a more potent inhibitor ofα-SMA and less potent inhibitor of COLⅠthan PGZ. But PGZ had the same effect as 15d-PGJ2 on CTGF. Conclusion PPARγagonist can prevent fibrosis induced by TGF-β1.

Citation: JINYan-wen, YEHui, XIONGXian-ze, LIFu-yu, ZHOURong-xing, ChengNan-sheng. Initial Study on Effect of Peroxisome Proliferator-Activated ReceptorγAgonist on Bile Duct Fibrosis Induced by Transforming Growth Factor-β1. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(7): 790-793. doi: 10.7507/1007-9424.20160208 Copy

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