Expression of miR-106a-5p in gastric cancer cells and gastric cancer tissue and analysis of enriched pathway of target genes_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 KANG Boxiong ¹ , LI Hailong ² , CHEN Che ² , MA Junfeng ¹ , FAN Yong ¹ , WANG Chen ¹

1. Department of General Surgery, The Second Hospital of Lanzhou University, Lanzhou 730030, P. R. China;
2. Department of Clinical Testing Teaching and Basic Research of Clinical Laboratory, School of Clinical Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, P. R. China;

Corresponding author：

KANG Boxiong, Email: oliverkang@163.com

Keywords：

miR-106a-5p gene; gastric cancer; tumor molecular marker

DOI：

10.7507/1007-9424.201712042

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To detect expression of miR-106a-5p in gastric cancer cells and gastric cancer tissue, and to analyze relationship between it’s expression with clinicopathologic characteristics, and in addition, to analyze its target genes and enriched pathway with bioinformatics method. Methods The expressions of miR-106a-5p in the different differentiation of gastric cancer cells AGS (well differentiation), MKN-28 (middle differentiation), HGC-27 (undifferentiation), MGC-803 (low differentiation), BGC-823 (low differentiation), MKN-45 (middle differentiation) and SGC-7901 (middle differentiation), the normal gastric mucosal epithelial cells GES-1, and the gastric cancer tissue and the corresponding adjacent tissue were detected by the real-time fluorescent quantitative PCR. Furthermore, the target genes of miR-106a-5p were predicted by using more than three softwares affiliated to mirWALK web database and the signal pathways of target genes were enriched by DAVID 6.7 software. Results The expressions of miR-106a-5p in the different differentiation degree of the gastric cancer cells (AGS, SGC-7901, MKN-45, MGC-803, BGC-823, and HGC-27) were up-regulated except the MKN-28 cell line as compared with the normal gastric mucosa cell line GES-1 (P<0.010 orP<0.001), and the expression of miR-106a-5p in the gastric cancer tissue was also up-regulated as compared with the corresponding adjacent tissue, the expression of miR-106a-5p in the gastric cancer tissue was associated with the lymph node metastasis or the invasion depth. The results of the bioinformatics analysis showed that the target genes of miR-106a-5p were enriched in the multiple signaling pathways associated with the cancer. Conclusion miR-106a-5p is a molecular marker of high expression in gastric cancer and a potential cancer gene associated with lymph node metastasis and invasion depth.

Citation： KANG Boxiong, LI Hailong, CHEN Che, MA Junfeng, FAN Yong, WANG Chen. Expression of miR-106a-5p in gastric cancer cells and gastric cancer tissue and analysis of enriched pathway of target genes. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(8): 923-928. doi: 10.7507/1007-9424.201712042 Copy

1.	Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin, 2016, 66(2): 115-132.
2.	杜亚琼, 姜波健, 俞继卫. miRNA在胃癌发生发展中的作用. 中国普外基础与临床杂志, 2016, 23(4): 499-502.
3.	Li X, Zhou Q, Tao L, et al. MicroRNA-106a promotes cell migration and invasion by targeting tissue inhibitor of matrix metalloproteinase 2 in cervical cancer. Oncol Rep, 2017, 38(3): 1774-1782.
4.	Chen L, Zhang F, Sheng XG, et al. MicroRNA-106a regulates phosphatase and tensin homologue expression and promotes the proliferation and invasion of ovarian cancer cells. Oncol Rep, 2016, 36(4): 2135-2141.
5.	李金虎, 赵晓俊, 张江磊, 等. miR-106a在人前列腺癌中的临床意义及生物学机制研究. 中国免疫学杂志, 2015, 31(7): 955-959.
6.	Xie X, Liu HT, Mei J, et al. miR-106a promotes growth and metastasis of non-small cell lung cancer by targeting PTEN. Int J Clin Exp Pathol, 2015, 8(4): 3827-3834.
7.	Ma HL, Wen XP, Zhang XZ, et al. miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1). Cell Mol Biol (Noisy-le-grand), 2015, 61(4): 56-62.
8.	Hao H, Xia G, Wang C, et al. miR-106a suppresses tumor cells death in colorectal cancer through targeting ATG7. Med Mol Morphol, 2017, 50(2): 76-85.
9.	Manne RK, Agrawal Y, Bargale A, et al. A MicroRNA/Ubiquitin ligase feedback loop regulates slug-mediated invasion in breast cancer. Neoplasia, 2017, 19(6): 483-495.
10.	Li D, Wang Z, Chen Z, et al. MicroRNA-106a-5p facilitates human glioblastoma cell proliferation and invasion by targeting adenomatosis polyposis coli protein. Biochem Biophys Res Commun, 2016, 481(3-4): 245-250.
11.	Zhu M, Zhang N, He S, et al. MicroRNA-106a targets TIMP2 to regulate invasion and metastasis of gastric cancer. FEBS Lett, 2014, 588(4): 600-607.
12.	Sierzega M, Kaczor M, Kolodziejczyk P, et al. Evaluation of serum microRNA biomarkers for gastric cancer based on blood and tissue pools profiling: the importance of miR-21 and miR-331. Br J Cancer, 2017, 117(2): 266-273.
13.	Zhu M, Zhang N, He S, et al. MicroRNA-106a functions as an oncogene in human gastric cancer and contributes to proliferation and metastasis in vitro and in vivo. Clin Exp Metastasis, 2016, 33(5): 509-519.
14.	李海龙, 宋耀辉, 陈兆峰, 等. miR-20a-5p/miR-20b-5p在胃癌细胞和组织中的表达及调控靶基因信号通路富集分析. 临床检验杂志, 2017, 35(11): 822-827.
15.	孔桂香, 黄晓俊, 金安琴, 等. 甘肃省河西地区33年胃镜检出胃癌的流行病学分析. 肿瘤防治研究, 2011, 38(12): 1438-1442.
16.	Wang Z, Liu M, Zhu H, et al. miR-106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS. Mol Carcinog, 2013, 52(8): 634-646.
17.	Pan YJ, Wei LL, Wu XJ, et al. MiR-106a-5p inhibits the cell migration and invasion of renal cell carcinoma through targeting PAK5. Cell Death Dis, 2017, 8(10): e3155.
18.	Luo B, Kang N, Chen Y, et al. Oncogene miR-106a promotes proliferation and metastasis of prostate cancer cells by directly targeting PTEN in vivo and in vitro. Minerva Med, 2018, 109(1): 24-30.
19.	He QY, Wang GC, Zhang H, et al. miR-106a-5p suppresses the proliferation, migration, and invasion of osteosarcoma cells by targeting HMGA2. DNA Cell Biol, 2016, 35(9): 506-520.
20.	Ma Y, Zhang H, He X, et al. miR-106a* inhibits the proliferation of renal carcinoma cells by targeting IRS-2. Tumour Biol, 2015, 36(11): 8389-8398.
21.	Zhi F, Zhou G, Shao N, et al. miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK. PLoS One, 2013, 8(8): e72390.
22.	Zhang Y, Lu Q, Cai X. MicroRNA-106a induces multidrug resistance in gastric cancer by targeting RUNX3. FEBS Lett, 2013, 587(18): 3069-3075.
23.	Yang G, Zhang R, Chen X, et al. MiR-106a inhibits glioma cell growth by targeting E2F1 independent of p53 status. J Mol Med (Berl), 2011, 89(10): 1037-1050.
24.	Zhou X, Zhu W, Li H, et al. Diagnostic value of a plasma microRNA signature in gastric cancer: a microRNA expression analysis. Sci Rep, 2015, 5: 11251.
25.	Tsujiura M, Ichikawa D, Komatsu S, et al. Circulating microRNAs in plasma of patients with gastric cancers. Br J Cancer, 2010, 102(7): 1174-1179.
26.	Wang JL, Hu Y, Kong X, et al. Candidate microRNA biomarkers in human gastric cancer: a systematic review and validation study. PLoS One, 2013, 8(9): e73683.

1. Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin, 2016, 66(2): 115-132.
2. 杜亚琼, 姜波健, 俞继卫. miRNA在胃癌发生发展中的作用. 中国普外基础与临床杂志, 2016, 23(4): 499-502.
3. Li X, Zhou Q, Tao L, et al. MicroRNA-106a promotes cell migration and invasion by targeting tissue inhibitor of matrix metalloproteinase 2 in cervical cancer. Oncol Rep, 2017, 38(3): 1774-1782.
4. Chen L, Zhang F, Sheng XG, et al. MicroRNA-106a regulates phosphatase and tensin homologue expression and promotes the proliferation and invasion of ovarian cancer cells. Oncol Rep, 2016, 36(4): 2135-2141.
5. 李金虎, 赵晓俊, 张江磊, 等. miR-106a在人前列腺癌中的临床意义及生物学机制研究. 中国免疫学杂志, 2015, 31(7): 955-959.
6. Xie X, Liu HT, Mei J, et al. miR-106a promotes growth and metastasis of non-small cell lung cancer by targeting PTEN. Int J Clin Exp Pathol, 2015, 8(4): 3827-3834.
7. Ma HL, Wen XP, Zhang XZ, et al. miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1). Cell Mol Biol (Noisy-le-grand), 2015, 61(4): 56-62.
8. Hao H, Xia G, Wang C, et al. miR-106a suppresses tumor cells death in colorectal cancer through targeting ATG7. Med Mol Morphol, 2017, 50(2): 76-85.
9. Manne RK, Agrawal Y, Bargale A, et al. A MicroRNA/Ubiquitin ligase feedback loop regulates slug-mediated invasion in breast cancer. Neoplasia, 2017, 19(6): 483-495.
10. Li D, Wang Z, Chen Z, et al. MicroRNA-106a-5p facilitates human glioblastoma cell proliferation and invasion by targeting adenomatosis polyposis coli protein. Biochem Biophys Res Commun, 2016, 481(3-4): 245-250.
11. Zhu M, Zhang N, He S, et al. MicroRNA-106a targets TIMP2 to regulate invasion and metastasis of gastric cancer. FEBS Lett, 2014, 588(4): 600-607.
12. Sierzega M, Kaczor M, Kolodziejczyk P, et al. Evaluation of serum microRNA biomarkers for gastric cancer based on blood and tissue pools profiling: the importance of miR-21 and miR-331. Br J Cancer, 2017, 117(2): 266-273.
13. Zhu M, Zhang N, He S, et al. MicroRNA-106a functions as an oncogene in human gastric cancer and contributes to proliferation and metastasis in vitro and in vivo. Clin Exp Metastasis, 2016, 33(5): 509-519.
14. 李海龙, 宋耀辉, 陈兆峰, 等. miR-20a-5p/miR-20b-5p在胃癌细胞和组织中的表达及调控靶基因信号通路富集分析. 临床检验杂志, 2017, 35(11): 822-827.
15. 孔桂香, 黄晓俊, 金安琴, 等. 甘肃省河西地区33年胃镜检出胃癌的流行病学分析. 肿瘤防治研究, 2011, 38(12): 1438-1442.
16. Wang Z, Liu M, Zhu H, et al. miR-106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS. Mol Carcinog, 2013, 52(8): 634-646.
17. Pan YJ, Wei LL, Wu XJ, et al. MiR-106a-5p inhibits the cell migration and invasion of renal cell carcinoma through targeting PAK5. Cell Death Dis, 2017, 8(10): e3155.
18. Luo B, Kang N, Chen Y, et al. Oncogene miR-106a promotes proliferation and metastasis of prostate cancer cells by directly targeting PTEN in vivo and in vitro. Minerva Med, 2018, 109(1): 24-30.
19. He QY, Wang GC, Zhang H, et al. miR-106a-5p suppresses the proliferation, migration, and invasion of osteosarcoma cells by targeting HMGA2. DNA Cell Biol, 2016, 35(9): 506-520.
20. Ma Y, Zhang H, He X, et al. miR-106a* inhibits the proliferation of renal carcinoma cells by targeting IRS-2. Tumour Biol, 2015, 36(11): 8389-8398.
21. Zhi F, Zhou G, Shao N, et al. miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK. PLoS One, 2013, 8(8): e72390.
22. Zhang Y, Lu Q, Cai X. MicroRNA-106a induces multidrug resistance in gastric cancer by targeting RUNX3. FEBS Lett, 2013, 587(18): 3069-3075.
23. Yang G, Zhang R, Chen X, et al. MiR-106a inhibits glioma cell growth by targeting E2F1 independent of p53 status. J Mol Med (Berl), 2011, 89(10): 1037-1050.
24. Zhou X, Zhu W, Li H, et al. Diagnostic value of a plasma microRNA signature in gastric cancer: a microRNA expression analysis. Sci Rep, 2015, 5: 11251.
25. Tsujiura M, Ichikawa D, Komatsu S, et al. Circulating microRNAs in plasma of patients with gastric cancers. Br J Cancer, 2010, 102(7): 1174-1179.
26. Wang JL, Hu Y, Kong X, et al. Candidate microRNA biomarkers in human gastric cancer: a systematic review and validation study. PLoS One, 2013, 8(9): e73683.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of miR-106a-5p in gastric cancer cells and gastric cancer tissue and analysis of enriched pathway of target genes

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