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find Author "汪泱" 7 results
  • RESEARCH PROGRESS OF URINE-DERIVED STEM CELLS

    ObjectiveTo review the current progresses in purification strategies, biological characters, and the uses in tissue engineering of urine-derived stem cells (USCs). MethodsRecent relevant publications on the USCs were extensively reviewed, analyzed, and summarized. ResultsUSCs, usually isolated by adherence screening method, are a population of mesenchymal stem cells (MSCs)-like somatic stem cells possessing robust self-renew and multi-potential differentiation ability. Combined with using appropriate biomaterials and biological molecules, USCs can be used as a good cell source for tissue engineering. ConclusionAn alluring prospect exists on the USCs-related research. Further studies are required to investigate the origin, individual differences, and the therapeutic values of USCs.

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  • RESEARCH PROGRESS OF ENDOTHELIAL PROGENITOR CELLS DERIVED EXTRACELLULAR VESICLES

    ObjectiveTo review the current progresses in purification strategies, biological characters, and functions of endothelial progenitor cells (EPCs) derived extracellular vesicles (EVs) (EPC-EVs). MethodsRecent relevant publications on the EPC-EVs were extensively reviewed, analyzed, and summarized. ResultsEPC-EVs are usually isolated by differential centrifugation and exhibit a homogenous pattern of spheroid particles with a diameter ranging from 60 to 160 nm under transmission electron microscopy. EPC-EVs are positive for cell-surface markers of EPCs (CD31, CD34, and CD133), and negative for markers of platelets (P-selectin and CD42b) and monocytes (CD14). Recent studies have shown the effectiveness of EPC-EVs in ischemic injuries, anti-Thy1 glomerulonephritis, and cardiomyocyte hypertrophy, and also shown their predictive role in cardio-cerebral-vascular diseases. ConclusionAn alluring prospect exists on the EPC-EVs-related research. Further studies are required to decipher the composition of EPC-EVs and their precise role in pathophysiological processes, and to investigate the molecular mechanisms for their targeting and function.

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  • RESEARCH PROGRESS OF MECHANISMS OF MESENCHYMAL STEM CELLS-DERIVED EXOSOMES IN TISSUE REPAIR

    ObjectiveTo review the mechanisms of bioactive substances of mesenchymal stem cells-derived exosomes (MEX) in tissue repair and analyze the therapeutic values of MEX. MethodRecent relevant literature about MEX for tissue repair was extensively reviewed and analyzed. ResultsThe diameter of exosomes ranges from 30 to 100 nm which contain an abundance of bioactive substances, such as mRNA, microRNA, and protein. The majority of the exact bioactive substances in MEX, which are therapeutically beneficial to a wide range of diseases, are still unclear. ConclusionsBioactive substances contained in the MEX have repairing effect in tissue injury, which could provide a new insight for the clinical treatment of tissue damage. However, further studies are required to investigate the individual differences of MEX and the possible risk of accelerating cancer progression of MEX.

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  • microRNA-210 MODIFIED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS INDUCE CAPILLARY FORMATION

    Objective To construct human recombinant lentiviral expression vector of microRNA-210 (miR-210)and to explore the over-expression of miR-210 on the capillary formation in human umbilical vein endothelial cells 12 (HUVE-12). Methods The recombinant lentiviral expression vector of pGCSIL-green fluorescent protein (GFP)-pre-miR-210 wasconstructed by molecular cloning and transfected to HUVE-12 (LV-miR-210-GFP group), only pGCSIL-GFP was transfectedas control group (LV-GFP group). The miR-210 expression activity was evaluated by GFP reporter through fluorescencedetection and real-time fluorescent quantitative PCR. The ephrinA3 protein expression was measured by flow cytometry. Theconcentration of vascular endothelial growth factor (VEGF) in culture supernatant was determined by ELISA. The cells werecultured in 96-well culture plate coated with Matrigel to assess the abil ity of capillary formation. Results The recombinantplasmid pGCSIL-GFP-pre-miR-210 was confirmed by restriction endonuclease analysis and DNA sequencing. Fluorescencedetection showed that the fluorescence intensity of GFP was highest between 48 and 72 hours after transfection. Real-timefluorescent quantitative PCR showed that the miR-210 expression of LV-miR-210-GFP group was 9.72 times higher than thatin LV-GFP group (t= —11.10,P=0.00). Flow cytometry analysis showed that the positive cell rate of enphrinA3 in LV-miR-210-GFP group (12.52% ± 0.67%) was significantly lower than that in LV-GFP group (73.22% ± 1.45%) (t= —66.12,P=0.00).The concentration of VEGF in supernatant in LV-miR-210-GFP group was significantly higher than that in LV-GFP group[(305.29 ± 16.52) pg/mL vs. (42.52 ± 3.11) pg/mL, t= —27.06,P=0.00]. In vitro capillary-l ike formation assay showed that thenumber of capillaries was significantly larger in LV-miR-210-GFP group than in LV-GFP group (17.33 ± 6.33 vs. 6.33 ± 2.33,t= —2.83,P=0.04). Conclusion The recombinant lentiviral expression vector of miR-210 is constructed successfully andHUVE-12 over-expressing miR-210 can significantly increase the capillary formation, which facil itates further study on themolecular functions of miR-210 in angiogenesis.

    Release date:2016-08-31 04:23 Export PDF Favorites Scan
  • 支气管镜联合消化内镜成功治疗食管癌术后气管食管瘘和胃底胸膜瘘一例

    目的 探索气管食管瘘及胃底胸膜瘘的临床救治方法。方法 回顾分析我院呼吸与危重症医学科经支气管镜联合消化内镜成功治疗食管癌术后气管食管瘘和胃底胸膜瘘1例。结果 患者男,64岁,因“进食后胸痛3周余”于2021年6月21日入胸外科,23日胃镜示食管中段新生物,病理为中分化鳞癌,25日行胸腔镜下右胸腹两切口食道癌根治术。术后第4天患者出现胸痛伴发热,7月5日胸部CT示左侧液气胸,胸腔镜探查术置管引流后脱机困难,10日转入呼吸与危重症医学科,数字减影血管造影下介入和气管镜检查明确诊断气管食管瘘和胃底胸膜瘘,保守治疗1个月效果不佳,经多学科讨论后先行气管镜引导下气道Y型覆膜支架植入封堵术,后行胃镜下氩离子束凝固术烧灼+气管食管瘘和胃底胸膜瘘钛夹夹闭术。患者术后痰量减少,无发热,复查CT两肺感染和胸腔积液明显吸收,消化道造影未见钡餐外溢现象,患者顺利脱机堵管,拔除气切套管后康复出院,门诊随访病情稳定。结论 食管癌术后短期内同时出现气管食管瘘和胃底胸膜瘘,实属罕见,救治难度较大。该患者的成功救治说明支气管镜联合消化内镜是治疗气管食管瘘和胃底胸膜瘘的有效方法,值得借鉴和推广。

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  • 鹦鹉热衣原体肺炎的临床诊疗特点

    目的 探讨鹦鹉热衣原体肺炎的临床诊疗特点。方法 收集2020年9月—2021年12月由苏州大学附属第一医院和附属独墅湖医院呼吸与危重症医学科收治的12例鹦鹉热衣原体肺炎患者的临床资料,进行总结分析。结果 12例患者均以高热、咳嗽为主要表现起病,9例患者接触过鹦鹉,3例接触过活禽。12例患者淋巴细胞计数均不同程度降低,2例患者白细胞、中性粒细胞和降钙素原明显升高;C反应蛋白、红细胞沉降率和D-二聚体均明显升高,而白蛋白水平明显降低,4例患者出现了低钾血症。1例重症患者合并了呼吸衰竭、心力衰竭、肝衰竭、肾衰竭及轻度精神症状。胸部CT均表现为单一肺叶或多肺叶炎症渗出和实变,5例合并有少量胸腔积液。患者初始经验性治疗效果均不理想,支气管肺泡灌洗液宏基因组二代测序技术检测诊断鹦鹉热衣原体肺炎后,及时调整以四环素类药物和(或)喹诺酮类抗生素为基础的治疗方案后病情均好转出院。结论鹦鹉热衣原体肺炎临床相对少见,临床表现以及常规检查很难与其他社区获得性肺炎鉴别,易导致误诊和漏诊,应引起临床医生的重视,宏基因组二代测序技术在鹦鹉热衣原体肺炎诊疗方面具有重要指导意义,值得临床应用和推广。

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  • Clinical characteristics and influencing factors of lower respiratory tract infection of Acinetobacter baumannii in respiratory intensive care unit

    ObjectiveTo investigate clinical characteristics and influencing factors of lower respiratory tract infection of Acinetobacter baumannii (AB-LRTI) in respiratory intensive care unit (RICU).MethodsClinical data were collected from 204 RICU patients who were isolated Acinetobacter baumannii (AB). The bacteriological specimens were derived from sputum, bronchoscopic endotracheal aspiration, bronchoalveolar lavage fluid, pleural effusion and blood. The definition of bacterial colonization was based on the responsible criteria from Centers for Disease Control and Prevention/National Medical Safety Network (CDC/NHSN). The patients were divided into three groups as follows, AB colonization group (only AB was isolated, n=40); simple AB-LRTI group (only AB was isolated and defined as infection, n=63), AB with another bacteria LRTI group (AB and another pathogen were isolated simultaneously, n=101). The epidemiology, clinical characteristics and influencing factors of each group were analyzed and compared. ResultsCompared with the AB colonization group, the AB with another bacteria LRTI group had higher proportion of patients with immunosuppression, specimens from sputum and bronchoalveolar lavage fluid, more than 4 invasive procedures, 90-day mortality, white blood cell count >10×109/L (or <4×109/L), neutrophil percent >75% (or <40%), lymphocyte count <1.1×109/L, platelet count <100×109/L, albumin <30 g/L, high sensitivity C-reactive protein >10 mg/L, and neutrophil-to-lymphocyte ratio (NLR). The frequency of bronchoscopy and days of infusing carbapenem within 90 days before isolating AB, the Acute Physiology and Chronic Health Evaluation Ⅱ score, the proportion of patients with invasive mechanical ventilation and the duration of invasive mechanical ventilation in the AB with another pathogen LRTI group were higher than those in the AB colonization group (all P<0.05). Days of infusing carbapenem and β-lactams/β-lactamase inhibitors within 90 days before isolating AB, proportion of septic shock, NLR and 90-day mortality of the patients from the AB with another pathogen LRTI group were more than those in the simple AB-LRTI group (all P<0.05). After regression analysis, more than 4 invasive procedures, or immunosuppression, or with more days of infusing carbapenem within 90 days before isolating AB were all the independent risk factors for AB-LRTI.ConclusionsThere are significant differences in epidemiology, clinical symptoms and laboratory indicators between simple AB-LRTI, AB with another pathogen LRTI and AB colonization in RICU patients. For RICU patients, who suffered more than 4 invasive procedures, immunosuppression, or with more days of infusing carbapenem within 90 days before isolating AB, are more susceptible to AB-LRTI.

    Release date:2021-05-25 01:52 Export PDF Favorites Scan
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