west china medical publishers
Keyword
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Keyword "Th2" 9 results
  • The effects of inhaled prostaglandin E1 on Th1/Th2 lipopolysaccharideinduced acute lung injury in rats

    Objective To investigate the effects of inhaled prostaglandin E1 (PGE1)on Th1/Th2 polarity in rat model of lipopolysaccharide(LPS) induced acute lung injury(ALI).Methods Healthy adult male Wistar rats [weight (200±20)g] were randomly divided into normal control(NS) group,LPS group and PGE1 group.The model of ALI were established by injecting LPS of 5 mg/kg into caudal vein.The rats in PGE1 group inhaled aerosolized PGE1(2 μg/mL)for 30 minutes after LPS injection,then repeat the procedure 12 hours later. 1 h,6 h,12 h and 24 h after last PGE1 inhalation,enzyme linked immunosorbant assay (ELISA) was empolyed to measure the level of interferon-γ(IFN-γ)and interleukin-4(IL-4)in the serum and bronchoalveolar lavage fluid(BALF)and the ratio of IFN-γ/IL-4(Th1/Th2)was calculated.Pathological examination was made under light microscope.Results  Pathological examination of lung tissue demonstrated success ALI model.Compared to NS group,the ratio of IFN-γ/IL-4(Th1/Th2)both in serum and BALF in LPS group elevated significantly(Plt;0.01). PGE1 administration significantly decreased the ratio IFN-γ/IL-4 in serum after 6h(Plt;0.01)and in BALF at all time points(Plt;0.01).Conclusion  The imbalance of was found in the LPS induced ALI,inhaled PGE1 aerosol inhalation could restore Th1/Th2 cytokine balance in the rats model induced by LPS.

    Release date:2016-09-14 11:52 Export PDF Favorites Scan
  • Anti-acute Rejection Effect of Watery Extract of Caesalppinia Sappan L and Th1/Th2 Deviation

    Objective To explore immunosuppressive effect and sappan L (WECSL) in heart transplantation of rats. Methods mechanism of watery extract of caesalppinia Wistar rats (donor) heart allografts were transplanted into the abdomen of SD rats (receptor). Ninety-six SD rats were divided into four groups (24 rats in each group). Control group: olive oil(8ml/kg·d) treated; group A: cyclosporine A (CsA,5ml/kg·d) treated; groupB: WECSL(37.5g/kg·d) treated; group C: WECSL(25g/kg·d) plus semidose of CsA(2.5mg/kg·d) treated. Median survival time of heart allografts and the histological changes of allografts were examined. Messenger ribonucleic acid (mRNA) of interleukin-2(IL-2), interleukinf-10(IL-10) in the myocardium were determined by reverse transcription polymerase chain reaction (RT-PCR), serum level of IL-2 and IL-10 were determined by enzyme-linked immunosorbent assay (ELISA) at 3, 7 days after surgery. Results Compared with control group, median survival time of heart allografts in group A, group B, group C was prolonged (P〈0. 01), lymphocyte infiltration and myocyte necrosis were relieved, mRNA expression of IL-2 in allografts was lower, mRNA expression of IL-10 was higher (P〈0.01). The serum levels of IL-2 in group A 3,7days after surgery and in group B, group C 3 days after surgery was lower than that in control group (P〈0.01). The serum levels of IL-10 in group A 7days after surgery and in group B 3 days after surgery was higher than that in control group (P〈0. 05). Conclusion Acute rejection of rat heart transplantation can be effectively suppressed by WECSL, Th1 to Th2 polarization induced by WECSL is observed.

    Release date:2016-08-30 06:23 Export PDF Favorites Scan
  • The relationship between Th1/Th2 cytokines mRNA expression and immune tolerance to cardiac allografts in rats

    Objective To study the relationship between Th1/Th2 cytokines messenger ribonucleic acid (mRNA) expression and immune tolerance to cardiac allografts in rats. Methods Male DA rat hearts were transplanted to male Lewis rats using Ono’s model and randomly divided into three groups: control group, rejection group, and tolerance group (each group 10 rats). Mean survival time (MST), histological changes, mRNA expression level of Th1/Th2 cytokines interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-4(IL-4), interleukin-10(IL-10) were measured. Results MST (85.28±7.48 d) of heart allografts in tolerance group was significantly longer than that(7.33±1.03 d) in rejection group. Only a few inflammatory cells infiltrated in cardiac allografts in tolerance group. The mRNA expression of IL-2, IFN-γ (Th1 cytokines) in rejection group were much ber than those in control group, and in tolerance group were much lower; mRNA expression of IL-4, IL-10 (Th2 cytokines) in rejection group were much ...更多lower than those in control group,and in tolerance group were much ber than those in control group. Conclusions The dynamic equilibrium of Th1/Th2 cytokines is very important in immune tolerance. The deviation of Th1 to Th2 is one of the mechanisms in immune tolerance.

    Release date:2016-08-30 06:28 Export PDF Favorites Scan
  • Expression and significance of T-bet in experimental autoimmune uveoretinitis

    Objective To investigate the expression and significance of T-bet in experimental autoimmune uveoretinitis (EAU). Methods EAU was induced in 24 Lewis rats by immunization with retinal S-antigen (50 μg) and complete Freund′s adjuvant, and another 4 rats were the healthy control. The rats with EAU were executed 7, 12, 15, 21 days after immunization, respectively. Immunohistochemical single and double staining were performed using monoclonal antibodies of T-bet or CD4 on the ocular wholemounts and the consecutive sections of the eye and spleen from both 24 immunized Lewis rats and 4 normal controls. The positive cells were counted under the optic microscope and the data were analyzed by SPSS 11.0 statistic software. Results A few T-bet positive cells were observed in the normal ocular tissues and spleen. The expressions of T-bet in the iris, retina, and spleen increased 7 days after immunization, reached the peak at the 15th day, and decreased at the 21st day, which were higher than those in the control. Double staining on the consecutive sections revealed that most of the T-bet positive cells were positive for CD4 monoclona l antibody. Conclusion T-bet may affect the occurrence of EAU by activating Th1 cells. (Chin J Ocul Fundus Dis,2004,20:172-174)

    Release date:2016-09-02 05:58 Export PDF Favorites Scan
  • Intranasal Administration of Interleukin-27 Alleviates Airway Allergic Inflammation of Ovalbumin-induced Mouse Asthma Model via STAT1 Signal Pathway

    ObjectiveTo observe whether interleukin-27 (IL-27) intervention could diminish allergic airway inflammation of mouse asthma induced by ovalbumin (OVA) and to investigate the related molecular mechanisms. MethodsSixty female C57/6J mice were randomly divided into six groups, a control group, an asthma group, two IL-27 prevention groups and two IL-27 treatment groups. Based on being sensitized and challenged with OVA in the asthma model, two kinds of IL-27 intervention asthma models were set up, one of which was low-dose multiple prevention model, the other was high-dose few times treatment model. HE stain and inflammation score were done for the lungs. CD4+ T cells were purified from mice spleen and cultured under Th2 medium with/without IL-27. Interleukin-4 (IL-4) was measured by ELISA. CD4+ T cells were cultured under different stringent Th2 medium and stimulated by IL-27. The level of total signal transducer and activator of transcription-1 (STAT1) protein and phos-STAT1 were tested by Western blot. ResultsIn low-dose multiple prevention group, IL-27 inhibited inflammation around bronchial and vascular obviously, the inflammation score was lower than the asthma group (P < 0.05), while the treatment group had no obvious statistical significance (P > 0.05). IL-27 repressed Th2 differentiation of naïve CD4+ T cells which was independent of interferon-γand IL-10. This effect was via STAT1 signaling pathway. CD4+ T cells from asthma mice or cultured under high-IL-4 inducing medium were found impairment of STAT1 phosphorylation. ConclusionsIL-27 could inhibit Th2 differentiation of naïve CD4+ T cells, but not in already committed Th2-CD4+ T cells. The inhibition effect of IL-27 for airway inflammation is obvious in prevention group, while the treatment group shows obviously resistance to inhibitory effect of IL-27. Already committed Th2-CD4+ T cells existed in asthma airway might be the reason for IL-27 resistance.

    Release date: Export PDF Favorites Scan
  • RSV-Stimulated Rat Airway Epithelial Cells Activate Myeloid Dendritic Cells

    Objective Respiratory syncytial virus ( RSV) is a primary cause of lower respiratory tract infections in children, and is also the cause for the development of asthma primarily in infants. However,the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aimof this study was to examine the influence of RSV-infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells ( mDCs) . Methods Rat airway epithelial cells ( RAECs) were infected by RSV. Then RSV-infected RAECs were co-cultured with rat mDCs, and the expression of cytokine and maturation markers on mDCs were examined by real time PCR and flow cytometry. To confirm this functional mDC maturation, allergenic mixed lymphocyte reaction ( MLR) were performed. Results Wefound that functional maturation of mDCs was induced by RSV-treated RAECs, as shown by their enhanced levels of OX40L and thymus- and activation-regulated chemokine ( TARC) mRNAs, which increased the expressions of major histocompatibility complex II ( MHCII) and CD86 costimulatorymolecules and promotedT-cell proliferation in mixed lymphocyte reactions. Conclusion Our results suggest that RSV-infected epithelial cells promote the maturation of mDCs that might support Th2 cell polarization and contribute to the pathogenesis of asthma.

    Release date:2016-08-30 11:54 Export PDF Favorites Scan
  • 胸腺基质淋巴细胞生成素在哮喘Th2优势免疫中的作用

    支气管哮喘( 简称哮喘) 是以树突状细胞( DC) 介导的Ⅱ型辅助性T 细胞( Th2) 优势免疫为特征的慢性气道炎症性疾病。哮喘气道产生大量的促炎症细胞因子, 募集嗜酸粒细胞、肥大细胞和淋巴细胞等炎症细胞, 释放一系列炎症因子, 引起气道炎症、黏膜水肿、黏液分泌和血管扩张等过敏症状。其中, 来源于上皮细胞的胸腺基质淋巴细胞生成素( thymic stromal lymphopoietin, TSLP) 在哮喘患者体内高表达, 在Th1/Th2 免疫失衡中起重要作用, 业已引起广泛关注。尤其随着对TSLP和TSLP受体( TSLPR) 的成功克隆与测序, TSLP 活化DC、启动Th2 免疫应答的分子机制, 以及TSLP在哮喘炎症上游阶段中的功能逐渐被识别, 人们对哮喘的免疫学发病机制有了更新的认识。

    Release date:2016-08-30 11:55 Export PDF Favorites Scan
  • 敲除与Th2 细胞活化相关的电压依赖钙通道可防止实验性哮喘发生(Knocking down Cav1 calcium channels implicated in Th2 cell activation prevents experimental asthma)

    敲除与Th2 细胞活化相关的电压依赖钙通道可防止实验性哮喘发生(Knocking down Cav1 calcium channels implicated in Th2 cell activation prevents experimental asthma) 【摘要翻译】 研究理由: Th2 型细胞参与过敏性哮喘,这些细胞产生的细胞因子( IL-4、IL-5 及IL-13) 在过敏状态时分泌增加。因此, 研究Th2 型细胞表达的对其功能具有重要影响的关键信号分子至关重要。我们既往的研究显示二氢吡¤特异性调控Th2 细胞的功能。目的: 由于二氢吡¤可特异性与活化细胞的电压依赖钙通道( Cav1) 结合并调节其功能, 我们的主要目的是证实Th2 细胞特异性表达功能性的Cav1 相关通道, 抑制其功能可能抑制哮喘。方法: 我们通过定量PCR 和Western blot 检测Th2 和Th1 细胞Cav1 通道表达。我们将Th2 细胞表达的Cav1 的异构体进行测序, 并研究Cav1 反义寡核苷酸( Cav1AS) 是否影响Ca2 + 信号及细胞因子的产生。最后, 我们通过给OVA 鼻腔激发的BALB/c小鼠注射Cav1AS 转染的OVA 特异性Th2 细胞研究Cav1AS在被动免疫哮喘动物模型中的作用, 并通过鼻腔给予此前进行过OVA 及氢氧化铝免疫的BABL/ c 小鼠Cav1AS 和OVA溶液以研究Cav1AS 对主动免疫哮喘模型的影响。检测和主要结果: 我们发现小鼠Th2 细胞而非Th1 细胞表达Cav1. 2和Cav1. 3 通道。转染Cav1AS 抑制了钙通路和细胞因子产生, 并导致Th2 细胞丧失过继Th2 细胞诱导气道炎症功能。鼻腔内给予Cav1AS 可抑制主动免疫导致的哮喘气道炎症和气道高反应性。结论: 这些结果提示Th2 细胞特异性表达Cav1. 2 and Cav1. 3 通道, 以此作为治疗靶点可有效抑制动物模型的哮喘反应。 【述评】 哮喘是一种Th2 型慢性气道炎症反应疾病,目前机制未明。Th2 细胞在此过程中发挥关键作用, 但Th2细胞活化机制不清楚。本文的研究发现Th2 细胞特异性表达Cav1. 2 和Cav1. 3 通道, 以Cav1AS抑制Cav1 通道的表达可抑制Th2 细胞功能进而抑制哮喘炎症反应。该研究揭示了哮喘气道炎症反应的新机制, 为哮喘治疗提供了新靶点。但是, Cav1 通道如何影响Th2 细胞的功能尚需进一步研究。其次, 有些基因在人类和小鼠表达并不一致, 特别是一些异构体的表达水平不同, 甚至在功能上存在很大差异, 因此, 在哮喘患者中Cav1 通道的表达尚待研究。最后, Th2 功能失调在一些自身免疫性疾病中发挥重要作用, 因此, 如证实Cav1 通道可用于人类哮喘治疗, 则该方法可能对其他一些自身免疫性疾病有治疗作用。

    Release date:2016-08-30 11:54 Export PDF Favorites Scan
  • 难治性哮喘中Th1/Th2失衡机制

    【摘要】 支气管哮喘是一种由多种细胞包括气道的炎性细胞、结构细胞和细胞组分参与的气道慢性炎症性疾病。临床上少数患者通过充分的哮喘治疗包括使用全身性激素治疗后仍不能有效控制, 通常称为“难治性哮喘”。免疫反应在难治性哮喘发病机制中起重要作用,而Th1/Th2失衡贯穿于难治性哮喘发病的整个过程。现就难治性哮喘中Th1/Th2失衡机制,以及影响因子(干扰素-γ、白介素-12、白介素-4、白介素-17,白介素-33和转化生长因子-β等)的研究作一综述,以深入探讨难治性哮喘的发病机制。

    Release date:2016-09-08 09:26 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content